Detailed information    

experimental Experimentally validated

Overview

Name   pilU   Type   Machinery gene
Locus tag   A1552VC_RS01165 Genome accession   NZ_CP028894
Coordinates   220582..221688 (+) Length   368 a.a.
NCBI ID   WP_000422572.1    Uniprot ID   -
Organism   Vibrio cholerae strain A1552     
Function   type IV pilus retraction   
DNA binding and uptake

Function

In addition to demonstrating that PilU can function as a bona fide retraction ATPase, we go on to make the surprising discovery that PilU functions exclusively in a PilT-dependent manner and identify a naturally occurring pandemic V. cholerae PilT variant that renders PilU essential for pilus function.


Genomic Context

Location: 215582..226688
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A1552VC_RS01130 (A1552VC_00220) - 215873..216475 (-) 603 WP_000725019.1 XTP/dITP diphosphatase -
  A1552VC_RS01135 (A1552VC_00221) - 216545..216976 (-) 432 WP_001233674.1 DUF4426 domain-containing protein -
  A1552VC_RS01140 (A1552VC_00222) yggU 217022..217312 (-) 291 WP_000718491.1 DUF167 family protein YggU -
  A1552VC_RS01145 (A1552VC_00223) - 217312..217869 (-) 558 WP_001087263.1 YggT family protein -
  A1552VC_RS01150 (A1552VC_00224) proC 217923..218741 (-) 819 WP_000437941.1 pyrroline-5-carboxylate reductase -
  A1552VC_RS01155 (A1552VC_00225) - 218794..219504 (-) 711 WP_001256653.1 YggS family pyridoxal phosphate-dependent enzyme -
  A1552VC_RS01160 (A1552VC_00226) pilT 219530..220567 (+) 1038 WP_000350195.1 type IV pilus twitching motility protein PilT Machinery gene
  A1552VC_RS01165 (A1552VC_00227) pilU 220582..221688 (+) 1107 WP_000422572.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  A1552VC_RS01170 (A1552VC_00228) - 221806..222603 (+) 798 WP_001123226.1 helix-turn-helix transcriptional regulator -
  A1552VC_RS01175 (A1552VC_00229) tyrS 222712..223992 (+) 1281 WP_001088155.1 tyrosine--tRNA ligase -
  A1552VC_RS01180 (A1552VC_00230) ruvX 224030..224452 (-) 423 WP_000091869.1 Holliday junction resolvase RuvX -
  A1552VC_RS01185 (A1552VC_00231) - 224533..225096 (-) 564 WP_001054768.1 YqgE/AlgH family protein -
  A1552VC_RS01190 (A1552VC_00232) gshB 225132..226088 (-) 957 WP_000593313.1 glutathione synthase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41307.27 Da        Isoelectric Point: 6.5076

>NTDB_id=1177 A1552VC_RS01165 WP_000422572.1 220582..221688(+) (pilU) [Vibrio cholerae strain A1552]
MELNQYLDGMLTHKASDLYITVGAPILYRVDGELRAQGEALSVADVTALLHAMMDDARQAEFKQTREANFAVVRDSGRFR
VSAFFQRELPGAVIRRIETRIPTFEELKLPEVLQNLAIAKRGLVLVVGATGSGKSTTMAAMTGYRNQHRTGHILTVEDPI
EFVHEHKRCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQREQFLLDLSLNLKGVIAQQLLRDKNGKGRHGVFEVLLNSPRIADLIRRGELHELKATMARSQEVGMQTFDQALY
QLVVDDKISEQDALHSADSANDLRLMLKTKRGDDYGSGSLQNVKIDME

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=1177 A1552VC_RS01165 WP_000422572.1 220582..221688(+) (pilU) [Vibrio cholerae strain A1552]
ATGGAGTTGAATCAATATCTGGATGGCATGCTGACTCATAAAGCATCGGATCTGTACATTACGGTGGGTGCACCGATTTT
GTATCGAGTCGATGGTGAATTGCGTGCACAAGGTGAAGCGCTCAGCGTGGCGGATGTGACGGCCTTATTGCATGCGATGA
TGGATGACGCAAGGCAAGCAGAATTTAAGCAGACGCGCGAAGCGAACTTTGCTGTGGTGCGTGACAGTGGCCGTTTTCGG
GTCAGTGCATTTTTCCAACGTGAATTGCCGGGGGCGGTGATCCGCCGGATTGAAACGCGCATTCCCACCTTTGAAGAACT
CAAATTGCCTGAAGTGCTGCAAAATTTGGCGATTGCCAAACGCGGTTTAGTGTTGGTGGTTGGCGCAACCGGTTCGGGTA
AATCGACCACTATGGCAGCCATGACTGGCTATCGTAATCAGCATCGCACCGGGCATATTTTGACGGTCGAAGATCCCATT
GAGTTTGTGCATGAACACAAGCGCTGTATCGTGACGCAGCGAGAAGTGGGGCTTGATACCGAAAGTTATGAAGTGGCGCT
GAAAAACTCGCTGCGCCAAGCCCCCGATATGATTTTGATTGGTGAAATCCGCAGCCGAGAAACCATGGAATACGCGATGA
CTTTCGCGGAAACGGGGCATTTGTGTATGGCCACGCTGCACGCTAACAATGCTAACCAAGCGTTAGAGCGGATTTTGCAT
TTAGTGCCGAAAGAGCAGCGTGAGCAGTTTTTGCTCGATTTGTCACTCAACCTCAAAGGCGTGATTGCCCAGCAATTATT
GCGCGATAAAAATGGCAAAGGTCGACACGGGGTGTTTGAAGTGTTGCTCAATAGCCCGCGTATCGCGGATTTAATTCGCC
GCGGTGAACTGCATGAGCTGAAAGCGACCATGGCTCGTTCGCAGGAAGTGGGGATGCAAACTTTCGATCAAGCCTTATAC
CAGCTTGTGGTGGATGACAAAATCAGTGAGCAAGATGCCCTGCACAGTGCCGATTCAGCCAACGATCTGCGTTTGATGCT
CAAAACCAAGCGTGGTGATGACTACGGCAGCGGCTCTTTGCAGAATGTGAAGATTGATATGGAGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

58.857

95.109

0.56
  pilU Acinetobacter baylyi ADP1

54.701

95.38

0.522
  pilT Legionella pneumophila strain ERS1305867

42.773

98.547

0.422
  pilT Legionella pneumophila strain Lp02

42.773

98.547

0.422
  pilT Vibrio cholerae strain A1552

42.216

96.812

0.409
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.216

96.812

0.409
  pilT Acinetobacter baumannii D1279779

41.593

98.261

0.409
  pilT Acinetobacter nosocomialis M2

41.593

98.261

0.409
  pilT Acinetobacter baumannii strain A118

41.593

98.261

0.409
  pilT Pseudomonas aeruginosa PAK

41.298

98.547

0.407
  pilT Pseudomonas stutzeri DSM 10701

41.003

98.547

0.404
  pilT Acinetobacter baylyi ADP1

42.462

94.477

0.401
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.806

84.239

0.386
  pilT Neisseria gonorrhoeae MS11

38.329

100

0.383
  pilT Neisseria meningitidis 8013

38.551

99.424

0.383

Multiple sequence alignment    



References

[1] David W Adams et al. (2019) The type IV pilus protein PilU functions as a PilT-dependent retraction ATPase. PLoS Genetics 15(9):e1008393. [PMID: 31525185]