Detailed information    

experimental Experimentally validated

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACE17W_RS02630 Genome accession   NZ_CP169562
Coordinates   600746..601780 (-) Length   344 a.a.
NCBI ID   WP_010947729.1    Uniprot ID   Q5ZTZ5
Organism   Legionella pneumophila strain Lp02     
Function   power the assembly of type IV pilus   
DNA binding and uptake

Function

Deletion of comEC, encoding the putative IM DNA channel, pilQ, encoding the OM secretin, and pilT, encoding the retraction ATPase, resulted in undetectable levels of natural transformation in our assay.


Genomic Context

Location: 595746..606780
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACE17W_RS02610 (ACE17W_02610) rpoZ 597653..597856 (-) 204 WP_011214258.1 DNA-directed RNA polymerase subunit omega -
  ACE17W_RS02615 (ACE17W_02615) gmk 597907..598536 (-) 630 WP_010947726.1 guanylate kinase -
  ACE17W_RS02620 (ACE17W_02620) - 598542..599408 (-) 867 WP_010947727.1 YicC/YloC family endoribonuclease -
  ACE17W_RS02625 (ACE17W_02625) rph 599709..600416 (+) 708 WP_010947728.1 ribonuclease PH -
  ACE17W_RS02630 (ACE17W_02630) pilT 600746..601780 (-) 1035 WP_010947729.1 type IV pilus twitching motility protein PilT Machinery gene
  ACE17W_RS02635 (ACE17W_02635) - 601944..602639 (+) 696 WP_010947730.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACE17W_RS02640 (ACE17W_02640) proC 602636..603424 (+) 789 WP_010947731.1 pyrroline-5-carboxylate reductase -
  ACE17W_RS02645 (ACE17W_02645) - 603769..604344 (+) 576 WP_010947732.1 YggT family protein -
  ACE17W_RS02650 (ACE17W_02650) - 604404..605006 (+) 603 WP_011946920.1 DUF2845 domain-containing protein -
  ACE17W_RS02655 (ACE17W_02655) - 605020..605301 (+) 282 WP_010947734.1 hypothetical protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38386.27 Da        Isoelectric Point: 7.0290

>NTDB_id=1283 ACE17W_RS02630 WP_010947729.1 600746..601780(-) (pilT) [Legionella pneumophila strain Lp02]
MDIAELLAFSVKNKSSDLHISAGMPPMIRVDGDLRKINLPPLEHKDVIKIIYDIMNDRQRKEYEEFFETDFSFEIANLAR
FRVNAFNQSRGAAAVFRTIPSEILSMEDLGLPPIFKEMASFSRGLVLVTGPTGSGKSTTLAAVIDYINSSRYEHILTVED
PIEFVHQSKKCLVNQREVHKDTLSFNAALRSALREDPDIILVGELRDLETIRLAMTAAETGHLVFGTLHTNSATKTINRI
IDVFPAEEKSMVRSMLSESLQAVVAQSLLKKNKGGRVAALEIMMCTGAIRNLIREDKIAQMYSSIQTGQAKGMQTLDQHL
TELVNKNIISKHTAHEAALNKSLF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=1283 ACE17W_RS02630 WP_010947729.1 600746..601780(-) (pilT) [Legionella pneumophila strain Lp02]
ATGGATATCGCGGAATTATTGGCATTTTCAGTTAAAAACAAATCATCAGATTTGCATATTTCAGCTGGCATGCCTCCTAT
GATTCGTGTTGATGGTGATTTACGCAAAATTAATTTACCTCCATTAGAGCATAAAGACGTCATTAAGATTATTTATGACA
TAATGAATGACAGGCAAAGAAAAGAATATGAAGAATTTTTTGAAACAGATTTTTCCTTTGAAATAGCCAATCTGGCACGG
TTCAGGGTTAATGCTTTCAATCAATCGCGCGGTGCCGCTGCTGTTTTCAGAACTATTCCTTCTGAAATTCTTAGCATGGA
AGATTTAGGATTGCCCCCAATTTTTAAAGAAATGGCTAGTTTTTCCAGGGGTCTGGTTCTGGTTACTGGCCCAACTGGAT
CAGGAAAAAGTACCACGCTTGCAGCAGTCATCGATTATATTAATTCAAGTCGTTATGAGCATATTTTGACAGTTGAAGAT
CCCATTGAGTTTGTTCATCAAAGCAAAAAATGTCTGGTCAACCAACGCGAAGTACACAAAGACACATTGAGCTTTAATGC
CGCTTTGAGATCAGCGTTACGTGAAGATCCGGATATTATATTGGTGGGTGAATTACGCGATTTGGAAACTATACGCTTGG
CGATGACTGCAGCAGAAACCGGGCATTTGGTTTTTGGAACATTGCATACCAATTCTGCAACAAAAACAATAAACAGGATA
ATTGATGTATTTCCCGCTGAAGAAAAATCGATGGTACGCTCCATGCTTTCTGAATCATTGCAAGCTGTCGTTGCTCAAAG
TTTATTAAAAAAGAATAAAGGTGGTCGGGTTGCGGCGCTTGAAATTATGATGTGTACGGGGGCTATTCGTAATTTAATTC
GTGAGGATAAGATAGCGCAAATGTATTCGTCAATTCAAACTGGTCAAGCCAAAGGAATGCAAACATTGGATCAACATTTG
ACTGAATTGGTTAATAAAAATATTATTTCGAAACACACTGCTCATGAGGCTGCCCTGAACAAGTCATTATTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q5ZTZ5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Legionella pneumophila strain ERS1305867

100

100

1
  pilT Acinetobacter baumannii D1279779

75.581

100

0.756
  pilT Acinetobacter baumannii strain A118

75.581

100

0.756
  pilT Acinetobacter nosocomialis M2

75.581

100

0.756
  pilT Acinetobacter baylyi ADP1

73.256

100

0.733
  pilT Pseudomonas aeruginosa PAK

74.556

98.256

0.733
  pilT Pseudomonas stutzeri DSM 10701

74.26

98.256

0.73
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.477

95.64

0.645
  pilT Vibrio cholerae strain A1552

67.477

95.64

0.645
  pilT Neisseria meningitidis 8013

64.809

99.128

0.642
  pilT Neisseria gonorrhoeae MS11

64.516

99.128

0.64
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.275

100

0.494
  pilU Vibrio cholerae strain A1552

42.773

98.547

0.422
  pilU Pseudomonas stutzeri DSM 10701

41.791

97.384

0.407
  pilU Acinetobacter baylyi ADP1

40.361

96.512

0.39

Multiple sequence alignment    



References

[1] Sebastian A G Braus et al. (2022) The molecular basis of FimT-mediated DNA uptake during bacterial natural transformation. Nature Communications 13(1):1065. [PMID: 35246533]