Detailed information    

experimental Experimentally validated

Overview

Name   pilT   Type   Machinery gene
Locus tag   PAKAF_RS01995 Genome accession   NZ_LR657304
Coordinates   425059..426093 (+) Length   344 a.a.
NCBI ID   WP_003084552.1    Uniprot ID   A0A0H2ZKV1
Organism   Pseudomonas aeruginosa PAK     
Function   type IV pilus retraction   
DNA binding and uptake

Function

P. aeruginosa in biofilms is competent for natural transformation of both genomic and plasmid DNA. All T4P mutants were capable of some natural transformation of pUCPSK, however a significant reduction in transformation frequency compared to wild-type PAK was observed.


Genomic Context

Location: 420059..431093
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PAKAF_RS01970 (PAKAF_00394) - 420347..422314 (-) 1968 WP_016252881.1 dynamin-like GTPase family protein -
  PAKAF_RS01975 - 422422..422718 (-) 297 WP_031628111.1 DUF167 domain-containing protein -
  PAKAF_RS01980 (PAKAF_00396) - 422715..423308 (-) 594 WP_003155373.1 YggT family protein -
  PAKAF_RS01985 (PAKAF_00397) proC 423319..424140 (-) 822 WP_003142635.1 pyrroline-5-carboxylate reductase -
  PAKAF_RS01990 (PAKAF_00398) - 424152..424844 (-) 693 WP_003084549.1 YggS family pyridoxal phosphate-dependent enzyme -
  PAKAF_RS01995 (PAKAF_00399) pilT 425059..426093 (+) 1035 WP_003084552.1 type IV pilus twitching motility protein PilT Machinery gene
  PAKAF_RS02000 (PAKAF_00400) pilU 426271..427419 (+) 1149 WP_003100959.1 type IV pilus ATPase PilU Machinery gene
  PAKAF_RS02005 (PAKAF_00401) czcD 427427..428326 (-) 900 WP_003100957.1 CDF family zinc transporter CzcD -
  PAKAF_RS02010 (PAKAF_00402) - 428480..428884 (+) 405 WP_003084560.1 NINE protein -
  PAKAF_RS02015 (PAKAF_00403) - 429127..430500 (+) 1374 WP_003084562.1 pyridoxal-phosphate dependent enzyme -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38020.89 Da        Isoelectric Point: 6.8364

>NTDB_id=1194 PAKAF_RS01995 WP_003084552.1 425059..426093(+) (pilT) [Pseudomonas aeruginosa PAK]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPPLEHKQVHALIYDIMNDKQRKDFEEFLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEELGMGEVFKRVSDVPRGLVLVTGPTGSGKSTTLAAMLDYLNNTKYHHILTIED
PIEFVHESKKCLVNQREVHRDTLGFSEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAEEKAMVRSMLSESLQSVISQTLIKKIGGGRVAAHEIMIGTPAIRNLIREDKVAQMYSAIQTGGSLGMQTLDMCL
KGLVAKGLISRENAREKAKIPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=1194 PAKAF_RS01995 WP_003084552.1 425059..426093(+) (pilT) [Pseudomonas aeruginosa PAK]
ATGGATATTACCGAGCTGCTCGCCTTCAGTGCCAAACAGGGCGCTTCGGACCTGCACCTCTCCGCCGGCCTGCCACCCAT
GATCCGGGTGGATGGCGATGTACGCCGGATCAACCTGCCACCGCTGGAACACAAGCAGGTGCATGCGCTGATCTACGACA
TCATGAACGACAAGCAGCGCAAGGACTTCGAGGAATTCCTCGAGACCGACTTCTCCTTCGAGGTGCCGGGCGTCGCGCGT
TTCCGGGTCAACGCCTTCAACCAGAACCGTGGCGCCGGCGCGGTATTCCGGACCATTCCCTCCAAGGTACTGACCATGGA
GGAGCTTGGCATGGGAGAAGTGTTCAAACGTGTTTCAGACGTCCCGCGCGGGTTGGTACTGGTCACCGGGCCGACCGGTT
CGGGCAAGTCCACCACCCTGGCGGCGATGCTCGACTACCTGAACAACACCAAGTACCACCACATCCTCACCATCGAGGAC
CCGATCGAATTCGTCCACGAATCGAAGAAGTGCCTGGTCAACCAGCGCGAGGTGCATCGCGACACCCTCGGCTTCAGCGA
AGCGCTGCGCTCGGCGCTGCGGGAGGACCCGGACATCATCCTGGTCGGCGAGATGCGCGACCTGGAAACCATCCGCCTGG
CCCTTACCGCGGCGGAGACCGGCCACCTGGTATTCGGCACCCTGCACACCACCTCGGCGGCGAAGACCATCGACCGGGTG
GTCGACGTGTTCCCGGCCGAGGAAAAGGCCATGGTTCGCTCGATGCTCTCCGAGTCGCTGCAATCGGTGATCTCGCAGAC
CCTGATCAAGAAGATCGGCGGCGGCCGGGTGGCGGCCCACGAGATCATGATCGGCACCCCGGCGATCCGCAACCTGATCC
GCGAGGACAAGGTCGCGCAGATGTATTCGGCGATCCAGACCGGCGGCTCGCTGGGCATGCAGACCCTCGACATGTGCCTC
AAGGGCCTGGTCGCCAAGGGCCTGATCAGCCGCGAGAACGCCCGCGAGAAGGCGAAGATCCCGGAAAACTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2ZKV1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

94.186

100

0.942
  pilT Acinetobacter baumannii D1279779

84.302

100

0.843
  pilT Acinetobacter baumannii strain A118

84.302

100

0.843
  pilT Acinetobacter nosocomialis M2

84.012

100

0.84
  pilT Acinetobacter baylyi ADP1

81.686

100

0.817
  pilT Legionella pneumophila strain ERS1305867

74.556

98.256

0.733
  pilT Legionella pneumophila strain Lp02

74.556

98.256

0.733
  pilT Neisseria meningitidis 8013

66.667

100

0.669
  pilT Neisseria gonorrhoeae MS11

66.377

100

0.666
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

66.765

98.837

0.66
  pilT Vibrio cholerae strain A1552

66.765

98.837

0.66
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.464

100

0.526
  pilU Vibrio cholerae strain A1552

41.298

98.547

0.407
  pilU Pseudomonas stutzeri DSM 10701

40.896

97.384

0.398
  pilU Acinetobacter baylyi ADP1

39.306

100

0.395

Multiple sequence alignment    



References

[1] Laura M Nolan et al. (2020) Pseudomonas aeruginosa is capable of natural transformation in biofilms. Microbiology (Reading, England) 166(10):995-1003. [PMID: 32749953]