Detailed information    

experimental Experimentally validated

Overview

Name   pilT   Type   Machinery gene
Locus tag   PSJM300_01600 Genome accession   CP003725
Coordinates   343186..344220 (+) Length   344 a.a.
NCBI ID   AFN76400.1    Uniprot ID   -
Organism   Pseudomonas stutzeri DSM 10701     
Function   type IV pilus retraction   
DNA binding and uptake

Function

The pilT mutant was hyperpiliated but defective for further pilus-associated properties, such as twitching motility and plating of pilus-specific phage PO4.


Genomic Context

Location: 338186..349220
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSJM300_01580 - 338894..340861 (-) 1968 AFN76396.1 GTPase -
  PSJM300_01585 - 340977..341567 (-) 591 AFN76397.1 YGGT family protein -
  PSJM300_01590 - 341564..342391 (-) 828 AFN76398.1 pyrroline-5-carboxylate reductase -
  PSJM300_01595 - 342408..343118 (-) 711 AFN76399.1 alanine racemase domain-containing protein -
  PSJM300_01600 pilT 343186..344220 (+) 1035 AFN76400.1 twitching motility protein PilT Machinery gene
  PSJM300_01605 pilU 344278..345423 (+) 1146 AFN76401.1 twitching motility protein PilU Machinery gene
  PSJM300_01610 - 345477..346313 (-) 837 AFN76402.1 leucyl-tRNA synthetase -
  PSJM300_01615 - 346313..347524 (-) 1212 AFN76403.1 elongator protein -
  PSJM300_01620 - 347676..348074 (+) 399 AFN76404.1 hypothetical protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37946.67 Da        Isoelectric Point: 6.8360

>NTDB_id=1202 PSJM300_01600 AFN76400.1 343186..344220(+) (pilT) [Pseudomonas stutzeri DSM 10701]
MDITELLAFSAKQGASDLHLSSGLPPMIRVDGDVRRINLPAMDHKQVHALIYDIMNDKQRKDFEEFLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGMGEVFRKITDVPRGLVLVTGPTGSGKSTTLAAMLDYLNNTKYHHILTIED
PIEFVHESKKCLVNQREVHRDTLGFSEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAEEKSMVRSMLSESLQAVISQTLLKKVGGGRVAAHEIMIGTPAIRNLIREDKVAQMYSSIQTGGSLGMQTLDSCL
KGLLAKGLISRDSAKEKAKQPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=1202 PSJM300_01600 AFN76400.1 343186..344220(+) (pilT) [Pseudomonas stutzeri DSM 10701]
ATGGATATCACAGAACTGCTGGCCTTCAGCGCCAAGCAGGGTGCGTCGGACCTGCACCTTTCTTCCGGCCTGCCGCCGAT
GATCCGCGTCGACGGCGACGTGCGGCGGATCAATCTGCCGGCCATGGACCACAAGCAGGTGCATGCGCTGATCTACGACA
TCATGAACGACAAGCAGCGCAAGGATTTCGAGGAATTCCTCGAGACCGACTTCTCGTTCGAAGTGCCCGGCGTCGCCCGC
TTCCGGGTCAACGCCTTCAACCAGAACCGCGGTGCCGGCGCGGTATTCCGGACCATTCCGTCCAAGGTGCTGACCATGGA
GGATCTCGGCATGGGCGAGGTCTTCCGCAAGATCACCGACGTGCCGCGCGGCCTGGTGCTGGTCACCGGCCCGACCGGTT
CGGGCAAATCCACCACTCTGGCGGCGATGCTCGACTACCTGAACAACACCAAGTACCACCACATCCTCACCATCGAGGAC
CCGATCGAATTCGTCCACGAGTCGAAGAAGTGCCTGGTCAACCAGCGCGAGGTGCATCGCGACACCCTGGGCTTCTCCGA
AGCGCTGCGCTCGGCGCTGCGCGAAGACCCGGACATCATCCTGGTGGGTGAGATGCGCGATCTGGAAACCATCCGCCTGG
CGCTGACCGCCGCGGAGACCGGTCACCTGGTGTTCGGCACCCTGCACACCACTTCCGCCGCCAAGACCATCGACCGCGTG
GTCGACGTGTTCCCGGCCGAGGAGAAGTCCATGGTCCGCTCGATGCTCTCCGAGTCGTTGCAGGCGGTGATTTCCCAGAC
GCTGCTGAAGAAGGTCGGCGGCGGTAGGGTGGCGGCCCACGAGATCATGATCGGCACCCCGGCGATTCGTAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTATTCCTCGATCCAGACCGGTGGTTCGCTGGGCATGCAGACGCTCGACTCATGCCTC
AAGGGCCTGCTCGCCAAGGGCCTGATCTCGCGTGACAGCGCCAAGGAAAAGGCCAAGCAGCCGGAAAATTTTTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

94.186

100

0.942
  pilT Acinetobacter baumannii D1279779

84.012

100

0.84
  pilT Acinetobacter baumannii strain A118

84.012

100

0.84
  pilT Acinetobacter nosocomialis M2

83.721

100

0.837
  pilT Acinetobacter baylyi ADP1

83.43

100

0.834
  pilT Legionella pneumophila strain Lp02

74.26

98.256

0.73
  pilT Legionella pneumophila strain ERS1305867

74.26

98.256

0.73
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.843

97.965

0.674
  pilT Vibrio cholerae strain A1552

68.843

97.965

0.674
  pilT Neisseria meningitidis 8013

67.246

100

0.674
  pilT Neisseria gonorrhoeae MS11

66.957

100

0.672
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.464

100

0.526
  pilU Vibrio cholerae strain A1552

41.003

98.547

0.404
  pilU Pseudomonas stutzeri DSM 10701

40.896

97.384

0.398
  pilU Acinetobacter baylyi ADP1

39.017

100

0.392

Multiple sequence alignment    



References

[1] S Graupner et al. (2001) Requirement of novel competence genes pilT and pilU of Pseudomonas stutzeri for natural transformation and suppression of pilT deficiency by a hexahistidine tag on the type IV pilus protein PilAI. Journal of Bacteriology 183(16):4694-701. [PMID: 11466271]