Detailed information    

experimental Experimentally validated

Overview

Name   pilT   Type   Machinery gene
Locus tag   ABD1_RS04380 Genome accession   NC_020547
Coordinates   937484..938521 (-) Length   345 a.a.
NCBI ID   WP_000355489.1    Uniprot ID   -
Organism   Acinetobacter baumannii D1279779     
Function   assembly of type IV pilus   
DNA binding and uptake

Function

We examined the 33 knockout mutants by comparing their transformation efficiency with wild-type W068. No knockout strains were affected in growth, but 28 of these mutants were severely or completely impaired in natural transformability. These mutations were in 18 genes related to T4P (pilF, pilQ, tsaP, pilM, pilN, pilO, pilP, pilB, pilC, pilT, pilD, pilE, pilY2, pilY1, pilX, pilW, pilV, and fimU), six genes related to DNA uptake and processing (comEA, comA, comF, priA, dprA, and recA), two genes related to T2SS (xcpV and xcpW), and the crp and tonB2 genes.


Genomic Context

Location: 932484..943521
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABD1_RS04350 (ABD1_08370) - 933267..933722 (+) 456 WP_000782976.1 bacteriohemerythrin -
  ABD1_RS04355 (ABD1_08380) - 933875..934942 (+) 1068 WP_000092403.1 hypothetical protein -
  ABD1_RS04360 (ABD1_08390) - 934942..935265 (+) 324 WP_000442585.1 RnfH family protein -
  ABD1_RS04365 (ABD1_08400) - 935320..935718 (-) 399 WP_001170994.1 outer membrane protein assembly factor BamE -
  ABD1_RS04370 (ABD1_08410) fur 935830..936267 (+) 438 WP_001122847.1 ferric iron uptake transcriptional regulator -
  ABD1_RS04375 (ABD1_08420) pilU 936338..937456 (-) 1119 WP_000347036.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABD1_RS04380 (ABD1_08430) pilT 937484..938521 (-) 1038 WP_000355489.1 type IV pilus twitching motility protein PilT Machinery gene
  ABD1_RS04385 (ABD1_08440) - 938648..939340 (+) 693 WP_001108508.1 YggS family pyridoxal phosphate-dependent enzyme -
  ABD1_RS04390 (ABD1_08450) - 939542..943138 (-) 3597 WP_000698810.1 AAA family ATPase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38312.92 Da        Isoelectric Point: 6.7271

>NTDB_id=1060 ABD1_RS04380 WP_000355489.1 937484..938521(-) (pilT) [Acinetobacter baumannii D1279779]
MDITELLAFSVKNGASDLHLSAGMPPMIRVDGEVRRINLPALEHKDVHRLVYDIMNDKQRRDYEEKLETDFSFEVPNVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGLGQIFKDICDYPRGIVLVTGPTGSGKSTTLAAMLDYINENRYDHILTVED
PIEFVHQSKKCLINQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQTLLKKNGGGRVAAHEIMIGIPAIRNLIRENKVAQMYSAIQTGANHGMTTLDQSL
KGLVARGVISPQTARTAAKQPESFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=1060 ABD1_RS04380 WP_000355489.1 937484..938521(-) (pilT) [Acinetobacter baumannii D1279779]
ATGGATATTACAGAGCTACTCGCCTTCTCTGTGAAAAATGGCGCGTCCGATTTACATCTCTCTGCTGGCATGCCACCAAT
GATTCGTGTTGACGGTGAAGTTCGTCGTATTAACTTACCAGCCTTAGAACATAAAGATGTCCACCGTCTTGTTTACGACA
TCATGAATGATAAACAGCGCCGTGACTATGAAGAAAAACTCGAAACTGACTTTTCTTTTGAAGTACCTAATGTTGCGCGT
TTCCGTGTTAACGCATTTAACCAAAACCGTGGTGCTGGTGCAGTATTTCGTACCATTCCATCTAAAGTCCTCACCATGGA
AGATCTAGGTCTAGGACAAATCTTTAAAGATATTTGCGACTATCCTCGTGGCATTGTACTGGTGACAGGACCAACAGGTT
CGGGTAAATCAACTACGCTTGCAGCGATGCTCGATTATATTAACGAAAACCGCTATGACCATATTTTAACGGTGGAAGAT
CCAATTGAATTTGTACACCAGTCTAAGAAATGTCTCATTAACCAGCGTGAAGTGCATCGTGACACACACGGCTTTAATGA
AGCACTTCGCTCAGCACTGCGTGAAGACCCTGATATTATTTTGGTCGGTGAGATGCGTGACCTTGAAACCATTCGCTTGG
CATTAACCGCTGCCGAAACAGGTCACTTGGTGTTTGGTACGCTCCACACCACGTCGGCAGCAAAAACCATTGACCGTGTG
ATTGACGTATTCCCTGCCGAAGAAAAAGACATGGTTCGTGCCATGCTGTCTGAATCTTTACAAGCGGTTATTTCACAAAC
CCTGCTTAAAAAGAATGGCGGTGGCCGTGTCGCAGCCCATGAAATCATGATTGGTATTCCGGCTATTCGTAACTTAATTC
GTGAAAACAAAGTCGCGCAAATGTACTCAGCCATTCAAACTGGTGCCAATCATGGCATGACCACACTCGACCAAAGCTTA
AAAGGCTTAGTCGCTCGCGGTGTAATTAGCCCGCAAACCGCACGTACTGCGGCAAAACAGCCTGAATCATTCTTATAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii strain A118

100

100

1
  pilT Acinetobacter nosocomialis M2

99.71

100

0.997
  pilT Acinetobacter baylyi ADP1

88.953

100

0.89
  pilT Pseudomonas aeruginosa PAK

84.302

100

0.843
  pilT Pseudomonas stutzeri DSM 10701

84.012

100

0.84
  pilT Legionella pneumophila strain Lp02

75.581

100

0.756
  pilT Legionella pneumophila strain ERS1305867

75.581

100

0.756
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.726

97.391

0.699
  pilT Vibrio cholerae strain A1552

71.726

97.391

0.699
  pilT Neisseria meningitidis 8013

68.116

100

0.681
  pilT Neisseria gonorrhoeae MS11

67.826

100

0.678
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.174

100

0.522
  pilU Vibrio cholerae strain A1552

41.593

98.261

0.409
  pilU Pseudomonas stutzeri DSM 10701

40.597

97.101

0.394
  pilU Acinetobacter baylyi ADP1

38.728

100

0.388

Multiple sequence alignment    



References

[1] Yuan Hu et al. (2021) Natural Transformation in Acinetobacter baumannii W068: A Genetic Analysis Reveals the Involvements of the CRP, XcpV, XcpW, TsaP, and TonB2. Frontiers in Microbiology 12:738034. [PMID: 35126321]