Detailed information    

experimental Experimentally validated

Overview

Name   pilT   Type   Machinery gene
Locus tag   NMV_RS00245 Genome accession   NC_017501
Coordinates   53791..54834 (-) Length   347 a.a.
NCBI ID   WP_002218487.1    Uniprot ID   O85741
Organism   Neisseria meningitidis 8013     
Function   power the assembly of type IV pilus   
DNA binding and uptake

Function

When the pilT mutant was tested, we found that competence was abolished since no transformants could be detected. This finding confirms that PilT which powers pilus retraction is critical for DNA transformation, and suggests that the background level of transformation observed in the comP mutant was dependent on pilus retraction, and thus Tfp-dependent.


Genomic Context

Location: 48791..59834
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMV_RS00235 (NMV_0046) yccS 49966..52116 (-) 2151 WP_014573685.1 YccS family putative transporter -
  NMV_RS00240 (NMV_0047) - 52402..53628 (-) 1227 WP_002218483.1 PilT/PilU family type 4a pilus ATPase -
  NMV_RS00245 (NMV_0048) pilT 53791..54834 (-) 1044 WP_002218487.1 type IV pilus twitching motility protein PilT Machinery gene
  NMV_RS00250 (NMV_0049) - 54954..55640 (+) 687 WP_002237503.1 YggS family pyridoxal phosphate-dependent enzyme -
  NMV_RS00255 (NMV_0050) - 55640..56047 (+) 408 WP_002237504.1 hypothetical protein -
  NMV_RS00260 (NMV_0051) proC 56078..56887 (+) 810 WP_228372582.1 pyrroline-5-carboxylate reductase -
  NMV_RS00265 (NMV_0052) dksA 57035..57451 (+) 417 WP_002231430.1 RNA polymerase-binding protein DksA -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38049.53 Da        Isoelectric Point: 7.0893

>NTDB_id=1086 NMV_RS00245 WP_002218487.1 53791..54834(-) (pilT) [Neisseria meningitidis 8013]
MQITDLLAFGAKNKASDLHLSSGISPMIRVHGDMRRINLPEMSAEEVGNMVTSVMNDHQRKIYQQNLEVDFSFELPNVAR
FRVNAFNIGRGPAAVFRTIPSTVLSLEELKAPSIFQKIAESPRGMVLVTGPTGSGKSTTLAAMINYINETQPAHILTIED
PIEFVHQSKKSLINQRELHQHTLSFANALRSALREDPDVILVGEMRDPETIGLALTAAETGHLVFGTLHTTGAAKTVDRI
VDVFPAGEKEMVRSMLSESLTAVISQNLLKTHDGNGRVASHEILIANPAVRNLIRENKITQINSVLQTGQASGMQTMDQS
LQSLVRQGLIAPEVARRRAQNSESMSF

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=1086 NMV_RS00245 WP_002218487.1 53791..54834(-) (pilT) [Neisseria meningitidis 8013]
ATGCAGATTACCGACTTACTCGCCTTCGGCGCTAAAAACAAAGCATCCGACCTTCACCTGAGTTCGGGCATATCCCCTAT
GATTCGGGTTCACGGCGATATGCGGCGCATCAACCTTCCCGAAATGAGCGCGGAAGAGGTCGGTAATATGGTAACTTCGG
TGATGAACGACCACCAGCGGAAAATCTACCAGCAAAACTTGGAAGTTGACTTCTCGTTCGAACTGCCCAACGTCGCCCGA
TTCCGCGTCAACGCCTTCAACATCGGCCGCGGTCCCGCCGCCGTATTCCGCACCATTCCCAGCACCGTCTTATCGCTGGA
AGAATTGAAAGCCCCGAGCATTTTCCAAAAAATCGCAGAATCGCCGCGCGGCATGGTATTGGTTACCGGCCCTACCGGTT
CGGGCAAATCGACCACGCTTGCCGCAATGATCAACTACATCAACGAAACCCAGCCGGCACACATCCTGACCATCGAAGAC
CCGATCGAATTCGTCCATCAAAGCAAAAAATCCCTGATTAACCAGCGCGAGCTGCACCAGCACACCCTCAGCTTCGCCAA
CGCGCTGCGTTCCGCATTGCGCGAAGACCCCGACGTTATCCTTGTCGGCGAGATGCGCGACCCAGAAACCATTGGCTTGG
CACTGACCGCCGCCGAAACCGGACACTTGGTTTTCGGCACGCTGCACACGACCGGCGCAGCAAAAACCGTCGACCGTATT
GTGGACGTATTCCCGGCGGGAGAAAAAGAAATGGTGCGCTCTATGCTGTCCGAATCGCTGACCGCCGTCATCTCCCAAAA
CCTGCTGAAAACGCACGACGGCAACGGCCGTGTCGCCTCGCACGAAATCCTGATTGCCAACCCCGCCGTCCGCAACCTCA
TCCGCGAAAACAAAATCACGCAGATTAACTCCGTCCTGCAAACCGGGCAGGCGAGCGGTATGCAGACAATGGACCAATCG
CTGCAATCGCTGGTGCGCCAAGGGCTGATCGCACCGGAAGTCGCACGCAGACGCGCGCAAAACAGCGAAAGTATGAGTTT
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB O85741

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria gonorrhoeae MS11

99.135

100

0.991
  pilT Acinetobacter nosocomialis M2

68.116

100

0.681
  pilT Acinetobacter baumannii strain A118

68.116

100

0.681
  pilT Acinetobacter baumannii D1279779

68.116

100

0.681
  pilT Acinetobacter baylyi ADP1

67.536

100

0.677
  pilT Pseudomonas stutzeri DSM 10701

67.246

100

0.674
  pilT Pseudomonas aeruginosa PAK

66.667

100

0.669
  pilT Legionella pneumophila strain ERS1305867

64.809

99.128

0.642
  pilT Legionella pneumophila strain Lp02

64.809

99.128

0.642
  pilT Vibrio cholerae strain A1552

64.545

95.652

0.617
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

64.545

95.652

0.617
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.644

98.847

0.53
  pilU Pseudomonas stutzeri DSM 10701

40.29

99.424

0.401
  pilU Vibrio cholerae strain A1552

38.551

99.424

0.383
  pilU Acinetobacter baylyi ADP1

38.439

99.712

0.383

Multiple sequence alignment    



References

[1] Jamie-Lee Berry et al. (2013) Functional analysis of the interdependence between DNA uptake sequence and its cognate ComP receptor during natural transformation in Neisseria species. PLoS Genetics 9(12):e1004014. [PMID: 24385921]
[2] Daniel R Brown et al. (2010) Systematic functional analysis reveals that a set of seven genes is involved in fine-tuning of the multiple functions mediated by type IV pili in Neisseria meningitidis. Infection And Immunity 78(7):3053-63. [PMID: 20439474]
[3] Kazutoyo Yasukawa et al. (2006) Pilus-mediated adhesion of Neisseria meningitidis is negatively controlled by the pilus-retraction machinery. Molecular Microbiology 59(2):579-89. [PMID: 16390451]
[4] Etienne Carbonnelle et al. (2006) A systematic genetic analysis in Neisseria meningitidis defines the Pil proteins required for assembly, functionality, stabilization and export of type IV pili. Molecular Microbiology 61(6):1510-22. [PMID: 16968224]