Detailed information NTG0001307

Experimentally validated

Overview

• Name: pilT
• Type: Machinery gene
• Locus tag: DR_RS10055
• Genome accession: NC_001263
• Coordinates: 1982569..1983831 (-)
• Length: 420 a.a.
• NCBI ID: WP_010888596.1
• Uniprot ID: -
• Organism: Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539
• Function: assembly of type IV pilus DNA binding and uptake

Function

We identified the factors (PilQ, PilD, type IV pilins, PilB, PilT, ComEC-ComEA, and ComF) involved in DNA uptake and DNA translocation across the external and cytoplasmic membranes and showed that the DNA-uptake machinery is similar to that described in the Gram negative bacterium Vibrio cholerae.

Genomic Context

Location: 1977569..1988831

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DR_RS10025 (DR_1957)	-	1977773..1978219 (+)	447	WP_051618937.1	hypothetical protein	-
DR_RS10030 (DR_1958)	-	1978216..1979217 (+)	1002	WP_010888591.1	ADP-ribosylglycohydrolase family protein	-
DR_RS10035 (DR_1959)	-	1979214..1979771 (+)	558	WP_010888592.1	nucleotidyltransferase family protein	-
DR_RS10040 (DR_1960)	-	1979846..1981045 (+)	1200	WP_010888593.1	acetyl-CoA C-acetyltransferase	-
DR_RS10045 (DR_1961)	-	1981234..1981764 (+)	531	WP_010888594.1	ankyrin repeat domain-containing protein	-
DR_RS16865	-	1981662..1981916 (+)	255	WP_234944694.1	ankyrin repeat domain-containing protein	-
DR_RS10050 (DR_1962)	-	1981994..1982506 (+)	513	WP_010888595.1	hypothetical protein	-
DR_RS10055 (DR_1963)	-	1982569..1983825 (-)	1257	WP_034350111.1	type IV pilus twitching motility protein PilT	-
DR_RS10060 (DR_1964)	pilB	1983894..1986557 (-)	2664	WP_027479822.1	ATPase, T2SS/T4P/T4SS family	Machinery gene
DR_RS10065 (DR_1965)	-	1986737..1987261 (-)	525	WP_010888598.1	YqeG family HAD IIIA-type phosphatase	-
DR_RS10070 (DR_1966)	pgeF	1987258..1988019 (-)	762	WP_010888599.1	peptidoglycan editing factor PgeF	-

Sequence

Protein

• Length: 420 a.a.
• Molecular weight: 44834.13 Da
• Isoelectric Point: 6.7744

>NTDB_id=1307 DR_RS10055 WP_010888596.1 1982569..1983831(-) (pilT) [Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539]
MTSGIDITDILRLAADKGASDIILAAGLPPQFKLSGDYDAQGFEPLSASDTRKLMYSMMNERQQRIFEEKRELDFSFALG
DKARFRVNAFMQRGNVGGVMRLIPTTVRTIAEMGLPQTVIDIANAPRGLVLVTGPTGSGKSTTLASMIDHINTTKKLHIL
TIEDPIEFMHPNKASIINQREVGSDTLSFDDALRAALRQAPDVILVGEMRDYETIKAAVTAAETGHLVMGTLHTNSAPES
IDRIVDVFPEEQQEQIRVQLANNLVAVMTQQLLPRADGQGRVLAYEILLANPAVRALIREGKTFQITSVMQTGAREGMVT
MDAYLAGLFRRHLITYDKGLERAIDPKEFARLANDASAGLGGASMTPAAPPVPAYNSASSSSTGRGGDFGRGAAAPATPA
APAAGRSEPGRAGGFGRR

Nucleotide

• Length: 1263 bp

>NTDB_id=1307 DR_RS10055 WP_010888596.1 1982569..1983831(-) (pilT) [Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539]
ATGACCATGACTTCCGGCATCGACATCACCGATATCCTGCGCCTCGCCGCCGACAAGGGTGCCTCCGACATCATCCTGGC
CGCCGGGCTGCCGCCGCAGTTCAAACTCTCGGGCGACTACGACGCGCAGGGCTTCGAGCCGCTGAGCGCGAGTGACACCC
GCAAGCTGATGTACTCGATGATGAACGAGCGCCAGCAGCGCATCTTCGAGGAAAAGCGCGAACTCGACTTTTCCTTCGCG
CTCGGCGACAAGGCCCGCTTCCGCGTGAACGCCTTCATGCAGCGCGGCAACGTGGGCGGCGTGATGCGCCTCATCCCCAC
CACCGTCCGCACGATTGCCGAGATGGGGCTGCCGCAGACGGTGATCGACATCGCCAACGCGCCGCGCGGGCTGGTGCTCG
TCACCGGGCCGACGGGCTCGGGCAAATCGACCACCCTCGCCTCGATGATCGACCACATCAACACGACCAAGAAGCTGCAC
ATCCTGACCATCGAAGACCCCATCGAGTTCATGCACCCCAACAAGGCGAGCATCATCAACCAGCGCGAGGTCGGCAGCGA
CACCCTGAGCTTCGACGACGCCCTGCGCGCCGCGCTGCGTCAGGCCCCCGACGTGATTCTGGTGGGCGAAATGCGCGACT
ACGAGACCATCAAGGCCGCCGTGACCGCCGCCGAAACCGGGCACCTCGTGATGGGCACGCTGCACACCAACTCCGCGCCC
GAATCCATCGACCGCATCGTGGACGTGTTTCCCGAGGAGCAGCAGGAACAGATCCGGGTGCAGCTCGCCAACAACCTCGT
CGCCGTGATGACCCAGCAACTGCTGCCGCGCGCCGACGGGCAGGGACGCGTGCTGGCCTACGAAATCCTGCTGGCGAACC
CGGCGGTCCGCGCCCTGATCCGCGAGGGCAAAACCTTCCAGATCACCAGCGTGATGCAGACCGGCGCCCGCGAAGGCATG
GTCACGATGGACGCCTACCTCGCCGGGCTGTTCCGCCGCCATCTCATCACCTACGACAAGGGGCTGGAACGCGCCATCGA
CCCCAAGGAATTCGCCCGTCTCGCCAACGACGCTTCGGCGGGCCTCGGCGGCGCCAGCATGACGCCCGCCGCGCCGCCCG
TTCCCGCCTACAACAGCGCTTCGAGCAGCAGCACTGGGCGTGGTGGTGACTTCGGACGCGGCGCGGCGGCCCCAGCGACG
CCCGCTGCCCCTGCCGCAGGCCGCAGCGAACCGGGACGCGCCGGAGGCTTTGGCCGCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (14-274)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilT	Neisseria meningitidis 8013	53.644	98.847	0.53
pilT	Pseudomonas aeruginosa PAK	52.464	100	0.526
pilT	Pseudomonas stutzeri DSM 10701	52.464	100	0.526
pilT	Acinetobacter baumannii D1279779	52.174	100	0.522
pilT	Acinetobacter nosocomialis M2	52.174	100	0.522
pilT	Acinetobacter baumannii strain A118	52.174	100	0.522
pilT	Neisseria gonorrhoeae MS11	53.392	97.695	0.522
pilT	Acinetobacter baylyi ADP1	51.884	100	0.52
pilT	Legionella pneumophila strain ERS1305867	49.275	100	0.494
pilT	Legionella pneumophila strain Lp02	49.275	100	0.494
pilT	Vibrio cholerae strain A1552	51.988	94.783	0.493
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	51.988	94.783	0.493
pilU	Pseudomonas stutzeri DSM 10701	43.658	88.976	0.388
pilU	Vibrio cholerae strain A1552	45.806	84.239	0.386
pilU	Acinetobacter baylyi ADP1	40.331	94.517	0.381

References

• [1]: Dhirendra Kumar Sharma et al. (2025) Surviving the storm: exploring the role of natural transformation in nutrition and DNA repair of stressed Deinococcus radiodurans. Applied And Environmental Microbiology 91(1):e0137124. [PMID: 39651863]
• [2]: Solenne Ithurbide et al. (2020) Natural Transformation in Deinococcus radiodurans: A Genetic Analysis Reveals the Major Roles of DprA, DdrB, RecA, RecF, and RecO Proteins. Frontiers in Microbiology 11:1253. [PMID: 32625182]