Detailed information    

experimental Experimentally validated

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACIAD_RS04205 Genome accession   NC_005966
Coordinates   892214..893365 (-) Length   383 a.a.
NCBI ID   WP_004922051.1    Uniprot ID   A0A7G2SB40
Organism   Acinetobacter baylyi ADP1     
Function   mediate the depolymerization of the type IV pilus   
DNA binding and uptake

Function

Two deassembly ATPases, PilT and PilU, are suggested to mediate the depolymerization of the pseudopilus in A. baylyi, thereby pulling the DNA through the OM.


Genomic Context

Location: 887214..898365
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACIAD_RS04175 (ACIAD0904) - 887935..888714 (+) 780 WP_004922069.1 GNAT family N-acetyltransferase -
  ACIAD_RS04180 (ACIAD0905) - 888822..889670 (+) 849 WP_026056952.1 class II glutamine amidotransferase -
  ACIAD_RS04185 (ACIAD0907) - 889769..890854 (+) 1086 WP_004922063.1 hypothetical protein -
  ACIAD_RS04190 (ACIAD0908) - 890851..891162 (+) 312 WP_004922060.1 RnfH family protein -
  ACIAD_RS04195 (ACIAD0909) - 891218..891616 (-) 399 WP_004922056.1 outer membrane protein assembly factor BamE -
  ACIAD_RS04200 (ACIAD0910) fur 891728..892168 (+) 441 WP_004922054.1 ferric iron uptake transcriptional regulator -
  ACIAD_RS04205 (ACIAD0911) pilU 892214..893365 (-) 1152 WP_004922051.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACIAD_RS04210 (ACIAD0912) pilT 893402..894436 (-) 1035 WP_011182164.1 type IV pilus twitching motility protein PilT Machinery gene
  ACIAD_RS04215 (ACIAD0913) - 894561..895250 (+) 690 WP_004922045.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACIAD_RS04220 (ACIAD0914) - 895387..896244 (+) 858 WP_011182166.1 undecaprenyl-diphosphate phosphatase -
  ACIAD_RS04225 (ACIAD0915) - 896340..897125 (-) 786 WP_004922040.1 transglutaminase-like domain-containing protein -

Sequence

Protein


Download         Length: 383 a.a.        Molecular weight: 43616.87 Da        Isoelectric Point: 6.6084

>NTDB_id=1019 ACIAD_RS04205 WP_004922051.1 892214..893365(-) (pilU) [Acinetobacter baylyi ADP1]
MDFNDLLNLMAKHKASDLFITAGVEPSLKINGEITPVSKTKLDGDMIAQLIDSITTQKQRQEFIETHECNFAILNREKNE
RFRVSAFQQRDMPGMVIRRIETKIPSPDDLHLPPILKELAMSKRGIIIFSGATGTGKSTSLASMIQHRNQHSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYALAFAETGHLVFATLHANNANQTLER
IIHFFDADRHQQIFMDLSLNLKAVISQQLIPTHDGQSRRAAVEVLINTPLLSDYIRQGEIYKIKELMARSGELGMQTFDQ
ALFDLYDHQHISYADALKHADSPNNLRLQIKLSEEANKPLDNRERMSEMERKMTFDGQRRNLK

Nucleotide


Download         Length: 1152 bp        

>NTDB_id=1019 ACIAD_RS04205 WP_004922051.1 892214..893365(-) (pilU) [Acinetobacter baylyi ADP1]
ATGGACTTTAATGATTTACTCAATTTGATGGCAAAACACAAAGCATCTGATTTGTTTATTACAGCAGGTGTAGAACCATC
TTTAAAAATTAACGGTGAAATTACCCCCGTCTCTAAAACCAAACTCGATGGAGATATGATCGCTCAGTTGATTGATTCGA
TTACAACGCAAAAGCAACGACAAGAATTCATTGAAACGCATGAATGTAATTTTGCCATCTTAAATCGTGAAAAAAACGAA
CGTTTCCGGGTGAGCGCATTTCAACAGCGTGACATGCCTGGTATGGTGATTCGTCGTATTGAGACCAAGATCCCCTCTCC
TGACGATTTACATCTACCTCCAATTTTAAAAGAACTGGCCATGAGCAAACGTGGCATTATTATTTTTTCTGGGGCAACTG
GTACAGGAAAATCCACGTCTTTGGCATCCATGATTCAACATCGTAATCAGCACTCAAAAGGTCATATTATTACTATTGAG
GATCCGATTGAATTTATACATGAGCATGCTGGATGTATTATCACTCAGCGTGAAGTCGGCATTGATACAGATTCTTTTGA
AATCGCGCTTAAAAATACGTTGCGCCAGGCGCCTGATGTGATTCTGATTGGTGAAATTCGCTCACGTGAAGTGATGGACT
ATGCTTTAGCTTTTGCAGAAACTGGCCATTTAGTATTTGCAACTTTACATGCCAATAATGCCAATCAAACCTTAGAACGG
ATTATTCACTTCTTTGATGCAGATCGACATCAGCAAATTTTTATGGATTTATCGCTCAATCTTAAAGCGGTGATTTCACA
ACAGCTTATTCCAACCCATGATGGTCAAAGTCGACGTGCTGCCGTCGAAGTGCTGATTAATACGCCTTTACTGTCAGATT
ACATTCGTCAAGGTGAAATCTATAAAATTAAGGAATTAATGGCTCGTTCTGGAGAACTTGGCATGCAAACCTTTGATCAG
GCACTTTTTGATTTATATGATCATCAGCACATCAGTTATGCAGATGCGCTCAAGCATGCAGACTCGCCTAATAATTTACG
TCTACAGATCAAGCTTTCGGAAGAAGCCAACAAACCCCTAGATAACAGAGAAAGAATGTCCGAAATGGAACGGAAAATGA
CTTTTGATGGTCAACGCCGTAATCTCAAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7G2SB40

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

65.266

93.701

0.612
  pilU Vibrio cholerae strain A1552

54.701

95.38

0.522
  pilT Pseudomonas aeruginosa PAK

39.306

100

0.395
  pilT Pseudomonas stutzeri DSM 10701

39.017

100

0.392
  pilT Legionella pneumophila strain ERS1305867

40.361

96.512

0.39
  pilT Legionella pneumophila strain Lp02

40.361

96.512

0.39
  pilT Acinetobacter nosocomialis M2

38.728

100

0.388
  pilT Acinetobacter baumannii strain A118

38.728

100

0.388
  pilT Acinetobacter baumannii D1279779

38.728

100

0.388
  pilT Acinetobacter baylyi ADP1

38.439

100

0.387
  pilT Neisseria meningitidis 8013

38.439

99.712

0.383
  pilT Neisseria gonorrhoeae MS11

38.439

99.712

0.383
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.331

94.517

0.381
  pilT Vibrio cholerae strain A1552

40.625

92.754

0.377
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.625

92.754

0.377

Multiple sequence alignment    



References

[1] Beate Averhoff et al. (2021) Natural transformation in Gram-negative bacteria thriving in extreme environments: from genes and genomes to proteins, structures and regulation. Extremophiles : Life Under Extreme Conditions 25(5-6):425-436. [PMID: 34542714]
[2] Colleen G Leong et al. (2017) The role of core and accessory type IV pilus genes in natural transformation and twitching motility in the bacterium Acinetobacter baylyi. PloS One 12(8):e0182139. [PMID: 28771515]