Detailed information    

experimental Experimentally validated

Overview

Name   pilT   Type   Machinery gene
Locus tag   GTF74_RS11940 Genome accession   NZ_CP047303
Coordinates   2635880..2636917 (-) Length   345 a.a.
NCBI ID   WP_000350195.1    Uniprot ID   Q9KUQ3
Organism   Vibrio cholerae O1 biovar El Tor strain E7946     
Function   type IV pilus retraction   
DNA binding and uptake

Function

However, the pilT mutant exhibited a 10,000-fold reduction in transformation frequency and very little DNA internalization, despite exhibiting pilus retraction.


Genomic Context

Location: 2630880..2641917
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GTF74_RS11915 (GTF74_11920) - 2631351..2631914 (+) 564 WP_001054768.1 YqgE/AlgH family protein -
  GTF74_RS11920 (GTF74_11925) ruvX 2631995..2632417 (+) 423 WP_000091869.1 Holliday junction resolvase RuvX -
  GTF74_RS11925 (GTF74_11930) tyrS 2632455..2633735 (-) 1281 WP_001088155.1 tyrosine--tRNA ligase -
  GTF74_RS11930 (GTF74_11935) - 2633844..2634641 (-) 798 WP_001123226.1 helix-turn-helix transcriptional regulator -
  GTF74_RS11935 (GTF74_11940) pilU 2634759..2635865 (-) 1107 WP_000422572.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  GTF74_RS11940 (GTF74_11945) pilT 2635880..2636917 (-) 1038 WP_000350195.1 type IV pilus twitching motility protein PilT Machinery gene
  GTF74_RS11945 (GTF74_11950) - 2636943..2637653 (+) 711 WP_001256653.1 YggS family pyridoxal phosphate-dependent enzyme -
  GTF74_RS11950 (GTF74_11955) proC 2637706..2638524 (+) 819 WP_000437941.1 pyrroline-5-carboxylate reductase -
  GTF74_RS11955 (GTF74_11960) - 2638578..2639135 (+) 558 WP_001087263.1 YggT family protein -
  GTF74_RS11960 (GTF74_11965) yggU 2639135..2639425 (+) 291 WP_000718491.1 DUF167 family protein YggU -
  GTF74_RS11965 (GTF74_11970) - 2639471..2639902 (+) 432 WP_001233674.1 DUF4426 domain-containing protein -
  GTF74_RS11970 (GTF74_11975) - 2639972..2640574 (+) 603 WP_000725019.1 XTP/dITP diphosphatase -
  GTF74_RS11975 (GTF74_11980) hemW 2640568..2641743 (+) 1176 WP_000957351.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38231.83 Da        Isoelectric Point: 6.6672

>NTDB_id=1185 GTF74_RS11940 WP_000350195.1 2635880..2636917(-) (pilT) [Vibrio cholerae O1 biovar El Tor strain E7946]
MDIAELLEFSVKHNASDLHLSAGVPPMVRIDGEVRKLGVPAFTHSDVHRLIFEIMNDAQRSEYEEKLEVDFSFELPNVGR
FRVNAFHQARGCSAVFRTIPTVIPTLEQLDAPEIFSKIANYEKGLVLVTGPTGSGKSTTLAAMVNYVNAHHNKHILTIED
PIEFVHSNNKCLINQREVHRDTHSFKNALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRAVIAQKLLKRVGGGRVACHEIMLATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
KQLIARGVVDAQEVQSKIELDLKAF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=1185 GTF74_RS11940 WP_000350195.1 2635880..2636917(-) (pilT) [Vibrio cholerae O1 biovar El Tor strain E7946]
ATGGATATCGCTGAGTTACTGGAATTTAGTGTAAAACATAACGCCTCAGATCTACATCTTTCCGCAGGTGTTCCGCCTAT
GGTACGGATTGATGGCGAAGTTAGGAAGCTTGGCGTGCCTGCTTTTACCCATTCTGATGTACATCGCTTGATTTTTGAGA
TCATGAATGATGCCCAGCGCAGTGAATATGAAGAGAAATTAGAAGTCGATTTTTCTTTTGAACTGCCTAATGTTGGCCGT
TTCCGGGTTAACGCGTTTCATCAAGCGCGCGGATGCTCGGCGGTGTTTCGTACCATTCCCACTGTCATCCCGACGCTAGA
GCAACTGGATGCTCCTGAAATCTTCAGCAAAATTGCCAATTATGAAAAAGGGTTAGTTTTAGTGACTGGGCCAACGGGCT
CGGGTAAGTCGACGACCTTGGCGGCGATGGTGAACTATGTCAATGCTCATCACAATAAGCATATTTTGACCATTGAAGAT
CCGATTGAATTTGTGCACAGCAACAATAAGTGTTTGATCAACCAACGCGAAGTGCACCGTGATACCCACAGTTTTAAAAA
TGCATTGCGCTCGGCATTGCGTGAAGACCCAGATGTGATCCTCGTCGGTGAGCTGCGTGACCAAGAAACGATTAGCTTGG
CGCTTACTGCGGCAGAAACCGGTCACTTGGTATTTGGCACTCTGCACACCAGCTCGGCGGCGAAAACCATTGACCGGATT
ATCGATGTTTTTCCGGGCAGCGACAAAGACATGGTGCGTTCCATGTTGTCGGAATCGCTGCGTGCGGTTATTGCGCAAAA
ACTCCTAAAACGCGTCGGGGGTGGCCGTGTTGCGTGTCATGAAATCATGCTGGCGACGCCGGCAATTCGGAACTTGATCC
GTGAAGATAAAGTGGCGCAGATGTATTCGATCATCCAAACCGGTGCCGCACACGGCATGCAAACCATGGAGCAAAACGCC
AAACAGCTGATCGCGCGTGGTGTGGTGGATGCGCAAGAAGTACAGAGCAAAATCGAGTTGGATTTAAAAGCATTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9KUQ3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

100

100

1
  pilT Acinetobacter baumannii D1279779

71.726

97.391

0.699
  pilT Acinetobacter baumannii strain A118

71.726

97.391

0.699
  pilT Acinetobacter nosocomialis M2

71.429

97.391

0.696
  pilT Acinetobacter baylyi ADP1

72.424

95.93

0.695
  pilT Pseudomonas stutzeri DSM 10701

68.843

97.965

0.674
  pilT Pseudomonas aeruginosa PAK

66.765

98.837

0.66
  pilT Legionella pneumophila strain ERS1305867

67.477

95.64

0.645
  pilT Legionella pneumophila strain Lp02

67.477

95.64

0.645
  pilT Neisseria meningitidis 8013

64.545

95.652

0.617
  pilT Neisseria gonorrhoeae MS11

64.242

95.652

0.614
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.988

94.783

0.493
  pilU Vibrio cholerae strain A1552

42.216

96.812

0.409
  pilU Pseudomonas stutzeri DSM 10701

40.828

97.971

0.4
  pilU Acinetobacter baylyi ADP1

40.625

92.754

0.377

Multiple sequence alignment    



References

[1] Courtney K Ellison et al. (2018) Retraction of DNA-bound type IV competence pili initiates DNA uptake during natural transformation in Vibrio cholerae. Nature Microbiology 3(7):773-780. [PMID: 29891864]