Detailed information    

experimental Experimentally validated

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACIAD_RS04210 Genome accession   NC_005966
Coordinates   893402..894436 (-) Length   344 a.a.
NCBI ID   WP_011182164.1    Uniprot ID   A0A7G2SBU6
Organism   Acinetobacter baylyi ADP1     
Function   mediate the depolymerization of the type IV pilus   
DNA binding and uptake

Function

Two deassembly ATPases, PilT and PilU, are suggested to mediate the depolymerization of the pseudopilus in A. baylyi, thereby pulling the DNA through the OM.


Genomic Context

Location: 888402..899436
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACIAD_RS04180 (ACIAD0905) - 888822..889670 (+) 849 WP_026056952.1 class II glutamine amidotransferase -
  ACIAD_RS04185 (ACIAD0907) - 889769..890854 (+) 1086 WP_004922063.1 hypothetical protein -
  ACIAD_RS04190 (ACIAD0908) - 890851..891162 (+) 312 WP_004922060.1 RnfH family protein -
  ACIAD_RS04195 (ACIAD0909) - 891218..891616 (-) 399 WP_004922056.1 outer membrane protein assembly factor BamE -
  ACIAD_RS04200 (ACIAD0910) fur 891728..892168 (+) 441 WP_004922054.1 ferric iron uptake transcriptional regulator -
  ACIAD_RS04205 (ACIAD0911) pilU 892214..893365 (-) 1152 WP_004922051.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACIAD_RS04210 (ACIAD0912) pilT 893402..894436 (-) 1035 WP_011182164.1 type IV pilus twitching motility protein PilT Machinery gene
  ACIAD_RS04215 (ACIAD0913) - 894561..895250 (+) 690 WP_004922045.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACIAD_RS04220 (ACIAD0914) - 895387..896244 (+) 858 WP_011182166.1 undecaprenyl-diphosphate phosphatase -
  ACIAD_RS04225 (ACIAD0915) - 896340..897125 (-) 786 WP_004922040.1 transglutaminase-like domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38336.01 Da        Isoelectric Point: 6.2870

>NTDB_id=1020 ACIAD_RS04210 WP_011182164.1 893402..894436(-) (pilT) [Acinetobacter baylyi ADP1]
MDITELLAFSVQNRASDLHLSAGIPPMIRVDGEVRRINLPAMDHSEVHNLIYDIMNDKQRRDYEEKLETDFSFEVPNLAR
FRVNVFNQNRGAGAVFRTIPSQVLTLEELGLGKIFRDICDYPRGLVLVTGPTGSGKSTTLAAMLDYINNHRYDHILTVED
PIEFVHEPKKCLINQREVHRDTLGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
IDVFPAEEKDMVRTMLSESLQAVISQTLLKKEGGGRVAAHEIMIGIPAIRNLIREGKVAQMYSSIQTGATHGMTTLDQSL
KQLLAKGIINLPTARAVAKSPESF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=1020 ACIAD_RS04210 WP_011182164.1 893402..894436(-) (pilT) [Acinetobacter baylyi ADP1]
ATGGATATAACAGAATTACTCGCGTTTTCGGTGCAAAATCGTGCCTCTGACCTGCATTTATCCGCAGGAATTCCCCCCAT
GATCCGTGTCGATGGCGAAGTTCGTCGTATTAATCTTCCTGCAATGGATCATTCAGAAGTCCATAATCTCATTTACGATA
TTATGAATGATAAACAGCGCCGAGACTACGAAGAAAAACTGGAAACAGATTTTTCTTTTGAAGTGCCTAATCTAGCTCGT
TTTCGCGTCAATGTGTTTAATCAAAATCGTGGAGCTGGTGCTGTATTTCGGACCATTCCATCACAAGTTCTGACGCTTGA
AGAACTGGGACTGGGTAAAATTTTTCGGGATATTTGCGATTATCCGAGAGGACTCGTCCTTGTCACAGGCCCCACTGGTT
CCGGTAAATCAACCACCCTTGCAGCCATGCTCGATTATATTAACAATCATCGCTACGACCATATTTTGACTGTTGAAGAT
CCAATTGAGTTTGTACATGAGCCTAAAAAATGCCTGATCAATCAGCGTGAGGTACATCGCGATACATTAGGCTTCAATGA
AGCACTGCGCTCAGCTTTGCGTGAAGATCCAGACATTATTCTGGTTGGCGAAATGCGGGATCTGGAAACCATCCGTCTGG
CTCTTACTGCCGCAGAAACAGGGCATTTGGTTTTTGGAACACTGCATACCACATCAGCCGCGAAAACCATTGATCGTATT
ATTGATGTCTTTCCTGCCGAAGAAAAAGATATGGTTCGCACCATGTTGTCGGAATCCTTACAAGCGGTTATTTCCCAAAC
TTTATTAAAAAAAGAAGGCGGTGGACGTGTTGCAGCCCATGAAATCATGATTGGAATTCCTGCAATTCGCAATCTCATTC
GTGAAGGTAAAGTCGCTCAAATGTACTCTTCTATCCAAACAGGTGCGACACATGGCATGACGACCCTGGATCAGAGTCTC
AAACAGCTTTTGGCAAAAGGCATCATCAACTTACCAACGGCACGCGCTGTCGCAAAATCTCCAGAGTCGTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7G2SBU6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

88.953

100

0.89
  pilT Acinetobacter baumannii strain A118

88.953

100

0.89
  pilT Acinetobacter nosocomialis M2

88.663

100

0.887
  pilT Pseudomonas stutzeri DSM 10701

83.43

100

0.834
  pilT Pseudomonas aeruginosa PAK

81.686

100

0.817
  pilT Legionella pneumophila strain ERS1305867

73.256

100

0.733
  pilT Legionella pneumophila strain Lp02

73.256

100

0.733
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

72.424

95.93

0.695
  pilT Vibrio cholerae strain A1552

72.424

95.93

0.695
  pilT Neisseria meningitidis 8013

67.536

100

0.677
  pilT Neisseria gonorrhoeae MS11

67.246

100

0.674
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.884

100

0.52
  pilU Vibrio cholerae strain A1552

42.462

94.477

0.401
  pilU Acinetobacter baylyi ADP1

38.439

100

0.387
  pilU Pseudomonas stutzeri DSM 10701

39.701

97.384

0.387

Multiple sequence alignment    



References

[1] Beate Averhoff et al. (2021) Natural transformation in Gram-negative bacteria thriving in extreme environments: from genes and genomes to proteins, structures and regulation. Extremophiles : Life Under Extreme Conditions 25(5-6):425-436. [PMID: 34542714]
[2] Colleen G Leong et al. (2017) The role of core and accessory type IV pilus genes in natural transformation and twitching motility in the bacterium Acinetobacter baylyi. PloS One 12(8):e0182139. [PMID: 28771515]