Detailed information    

experimental Experimentally validated

Overview

Name   pilT   Type   Machinery gene
Locus tag   FDQ49_RS05335 Genome accession   NZ_CP040105
Coordinates   1151579..1152616 (+) Length   345 a.a.
NCBI ID   WP_006581387.1    Uniprot ID   A0A0A7XL61
Organism   Acinetobacter nosocomialis M2     
Function   assembly of type IV pilus   
DNA binding and uptake

Function

We constructed strains containing mutations in the pilA, pilD, or pilT gene and then determined the transformation efficiency of each strain. The transformation efficiency for all mutant strains was below the level of detection in our assay.


Genomic Context

Location: 1146579..1157616
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FDQ49_RS05325 (FDQ49_05325) - 1149810..1150652 (-) 843 WP_022575104.1 undecaprenyl-diphosphate phosphatase -
  FDQ49_RS05330 (FDQ49_05330) - 1150760..1151452 (-) 693 WP_022575105.1 YggS family pyridoxal phosphate-dependent enzyme -
  FDQ49_RS05335 (FDQ49_05335) pilT 1151579..1152616 (+) 1038 WP_006581387.1 type IV pilus twitching motility protein PilT Machinery gene
  FDQ49_RS05340 (FDQ49_05340) pilU 1152644..1153762 (+) 1119 WP_002052019.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  FDQ49_RS05345 (FDQ49_05345) fur 1153832..1154269 (-) 438 WP_002052029.1 ferric iron uptake transcriptional regulator -
  FDQ49_RS05350 (FDQ49_05350) bamE 1154381..1154779 (+) 399 WP_001170994.1 outer membrane protein assembly factor BamE -
  FDQ49_RS05355 (FDQ49_05355) - 1154835..1155158 (-) 324 WP_002052062.1 RnfH family protein -
  FDQ49_RS05360 (FDQ49_05360) - 1155158..1156225 (-) 1068 WP_022575106.1 membrane protein -
  FDQ49_RS05370 (FDQ49_05370) - 1156378..1156833 (-) 456 WP_000782976.1 bacteriohemerythrin -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38328.92 Da        Isoelectric Point: 6.7271

>NTDB_id=1081 FDQ49_RS05335 WP_006581387.1 1151579..1152616(+) (pilT) [Acinetobacter nosocomialis M2]
MDITELLAFSVKNGASDLHLSAGMPPMIRVDGEVRRINLPALEHKDVHRLVYDIMNDKQRRDYEEKLETDFSFEVPNVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGLGQIFKDICDYPRGIVLVTGPTGSGKSTTLAAMLDYINENRYDHILTVED
PIEFVHQSKKCLINQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQTLLKKNGGGRVAAHEIMIGIPAIRNLIRENKVAQMYSAIQTGANHGMTTLDQSL
KGLVSRGVISPQTARTAAKQPESFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=1081 FDQ49_RS05335 WP_006581387.1 1151579..1152616(+) (pilT) [Acinetobacter nosocomialis M2]
ATGGATATTACAGAGCTACTCGCCTTCTCTGTGAAAAATGGCGCATCCGATTTGCACTTATCAGCTGGTATGCCACCAAT
GATTCGTGTCGATGGTGAAGTTCGTCGTATTAACTTGCCAGCCTTAGAACATAAAGATGTCCACCGCCTCGTTTATGACA
TCATGAATGATAAACAGCGTCGTGACTATGAAGAAAAACTCGAAACTGACTTTTCTTTTGAAGTACCGAATGTTGCGCGT
TTCCGTGTAAACGCATTTAACCAAAACCGTGGTGCTGGTGCAGTATTTCGTACCATTCCATCTAAAGTCCTCACCATGGA
AGATTTAGGCTTAGGTCAAATCTTTAAAGATATTTGCGACTATCCTCGCGGGATTGTACTGGTCACAGGACCAACAGGTT
CGGGTAAATCAACTACGCTTGCAGCAATGCTCGATTATATTAATGAAAACCGTTATGACCATATTTTAACGGTGGAAGAT
CCAATCGAATTTGTACACCAGTCTAAGAAATGTCTGATTAACCAGCGTGAAGTGCATCGTGATACACATGGCTTTAATGA
AGCACTTCGCTCAGCACTACGTGAAGACCCAGATATTATTTTAGTCGGTGAGATGCGTGACCTTGAAACGATTCGATTAG
CACTTACTGCTGCCGAAACAGGTCACTTGGTATTTGGTACGCTTCACACCACATCGGCTGCAAAAACCATTGACCGTGTG
ATTGACGTATTCCCTGCTGAAGAAAAAGACATGGTTCGTGCCATGCTGTCTGAATCTTTACAAGCAGTTATTTCTCAGAC
CCTGCTTAAAAAGAATGGTGGTGGTCGTGTTGCGGCACATGAAATTATGATTGGTATTCCGGCTATTCGTAACTTAATTC
GTGAAAACAAAGTCGCACAAATGTATTCAGCTATTCAGACTGGTGCTAACCATGGCATGACAACCCTCGACCAAAGCCTG
AAAGGTTTGGTGTCACGTGGTGTAATTAGTCCACAAACAGCCCGTACAGCGGCGAAACAGCCTGAATCATTCCTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0A7XL61

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

99.71

100

0.997
  pilT Acinetobacter baumannii strain A118

99.71

100

0.997
  pilT Acinetobacter baylyi ADP1

88.663

100

0.887
  pilT Pseudomonas aeruginosa PAK

84.012

100

0.84
  pilT Pseudomonas stutzeri DSM 10701

83.721

100

0.837
  pilT Legionella pneumophila strain ERS1305867

75.581

100

0.756
  pilT Legionella pneumophila strain Lp02

75.581

100

0.756
  pilT Vibrio cholerae strain A1552

71.429

97.391

0.696
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.429

97.391

0.696
  pilT Neisseria meningitidis 8013

68.116

100

0.681
  pilT Neisseria gonorrhoeae MS11

67.826

100

0.678
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.174

100

0.522
  pilU Vibrio cholerae strain A1552

41.593

98.261

0.409
  pilU Pseudomonas stutzeri DSM 10701

40.597

97.101

0.394
  pilU Acinetobacter baylyi ADP1

38.728

100

0.388

Multiple sequence alignment    



References

[1] Christian M Harding et al. (2013) Acinetobacter baumannii strain M2 produces type IV pili which play a role in natural transformation and twitching motility but not surface-associated motility. MBio 4(4):e00360-13. [PMID: 23919995]