Detailed information    

experimental Experimentally validated

Overview

Name   pilT   Type   Machinery gene
Locus tag   A1552VC_RS01160 Genome accession   NZ_CP028894
Coordinates   219530..220567 (+) Length   345 a.a.
NCBI ID   WP_000350195.1    Uniprot ID   Q9KUQ3
Organism   Vibrio cholerae strain A1552     
Function   type IV pilus retraction   
DNA binding and uptake

Function

We showed that both traffic ATPases, PilB and PilT, were required for DNA uptake and efficient transformation of V. cholerae.


Genomic Context

Location: 214530..225567
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A1552VC_RS01125 (A1552VC_00219) hemW 214704..215879 (-) 1176 WP_000957351.1 radical SAM family heme chaperone HemW -
  A1552VC_RS01130 (A1552VC_00220) - 215873..216475 (-) 603 WP_000725019.1 XTP/dITP diphosphatase -
  A1552VC_RS01135 (A1552VC_00221) - 216545..216976 (-) 432 WP_001233674.1 DUF4426 domain-containing protein -
  A1552VC_RS01140 (A1552VC_00222) yggU 217022..217312 (-) 291 WP_000718491.1 DUF167 family protein YggU -
  A1552VC_RS01145 (A1552VC_00223) - 217312..217869 (-) 558 WP_001087263.1 YggT family protein -
  A1552VC_RS01150 (A1552VC_00224) proC 217923..218741 (-) 819 WP_000437941.1 pyrroline-5-carboxylate reductase -
  A1552VC_RS01155 (A1552VC_00225) - 218794..219504 (-) 711 WP_001256653.1 YggS family pyridoxal phosphate-dependent enzyme -
  A1552VC_RS01160 (A1552VC_00226) pilT 219530..220567 (+) 1038 WP_000350195.1 type IV pilus twitching motility protein PilT Machinery gene
  A1552VC_RS01165 (A1552VC_00227) pilU 220582..221688 (+) 1107 WP_000422572.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  A1552VC_RS01170 (A1552VC_00228) - 221806..222603 (+) 798 WP_001123226.1 helix-turn-helix transcriptional regulator -
  A1552VC_RS01175 (A1552VC_00229) tyrS 222712..223992 (+) 1281 WP_001088155.1 tyrosine--tRNA ligase -
  A1552VC_RS01180 (A1552VC_00230) ruvX 224030..224452 (-) 423 WP_000091869.1 Holliday junction resolvase RuvX -
  A1552VC_RS01185 (A1552VC_00231) - 224533..225096 (-) 564 WP_001054768.1 YqgE/AlgH family protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38231.83 Da        Isoelectric Point: 6.6672

>NTDB_id=1176 A1552VC_RS01160 WP_000350195.1 219530..220567(+) (pilT) [Vibrio cholerae strain A1552]
MDIAELLEFSVKHNASDLHLSAGVPPMVRIDGEVRKLGVPAFTHSDVHRLIFEIMNDAQRSEYEEKLEVDFSFELPNVGR
FRVNAFHQARGCSAVFRTIPTVIPTLEQLDAPEIFSKIANYEKGLVLVTGPTGSGKSTTLAAMVNYVNAHHNKHILTIED
PIEFVHSNNKCLINQREVHRDTHSFKNALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRAVIAQKLLKRVGGGRVACHEIMLATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
KQLIARGVVDAQEVQSKIELDLKAF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=1176 A1552VC_RS01160 WP_000350195.1 219530..220567(+) (pilT) [Vibrio cholerae strain A1552]
ATGGATATCGCTGAGTTACTGGAATTTAGTGTAAAACATAACGCCTCAGATCTACATCTTTCCGCAGGTGTTCCGCCTAT
GGTACGGATTGATGGCGAAGTTAGGAAGCTTGGCGTGCCTGCTTTTACCCATTCTGATGTACATCGCTTGATTTTTGAGA
TCATGAATGATGCCCAGCGCAGTGAATATGAAGAGAAATTAGAAGTCGATTTTTCTTTTGAACTGCCTAATGTTGGCCGT
TTCCGGGTTAACGCGTTTCATCAAGCGCGCGGATGCTCGGCGGTGTTTCGTACCATTCCCACTGTCATCCCGACGCTAGA
GCAACTGGATGCTCCTGAAATCTTCAGCAAAATTGCCAATTATGAAAAAGGGTTAGTTTTAGTGACTGGGCCAACGGGCT
CGGGTAAGTCGACGACCTTGGCGGCGATGGTGAACTATGTCAATGCTCATCACAATAAGCATATTTTGACCATTGAAGAT
CCGATTGAATTTGTGCACAGCAACAATAAGTGTTTGATCAACCAACGCGAAGTGCACCGTGATACCCACAGTTTTAAAAA
TGCATTGCGCTCGGCATTGCGTGAAGACCCAGATGTGATCCTCGTCGGTGAGCTGCGTGACCAAGAAACGATTAGCTTGG
CGCTTACTGCGGCAGAAACCGGTCACTTGGTATTTGGCACTCTGCACACCAGCTCGGCGGCGAAAACCATTGACCGGATT
ATCGATGTTTTTCCGGGCAGCGACAAAGACATGGTGCGTTCCATGTTGTCGGAATCGCTGCGTGCGGTTATTGCGCAAAA
ACTCCTAAAACGCGTCGGGGGTGGCCGTGTTGCGTGTCATGAAATCATGCTGGCGACGCCGGCAATTCGGAACTTGATCC
GTGAAGATAAAGTGGCGCAGATGTATTCGATCATCCAAACCGGTGCCGCACACGGCATGCAAACCATGGAGCAAAACGCC
AAACAGCTGATCGCGCGTGGTGTGGTGGATGCGCAAGAAGTACAGAGCAAAATCGAGTTGGATTTAAAAGCATTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9KUQ3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

100

100

1
  pilT Acinetobacter baumannii D1279779

71.726

97.391

0.699
  pilT Acinetobacter baumannii strain A118

71.726

97.391

0.699
  pilT Acinetobacter nosocomialis M2

71.429

97.391

0.696
  pilT Acinetobacter baylyi ADP1

72.424

95.93

0.695
  pilT Pseudomonas stutzeri DSM 10701

68.843

97.965

0.674
  pilT Pseudomonas aeruginosa PAK

66.765

98.837

0.66
  pilT Legionella pneumophila strain ERS1305867

67.477

95.64

0.645
  pilT Legionella pneumophila strain Lp02

67.477

95.64

0.645
  pilT Neisseria meningitidis 8013

64.545

95.652

0.617
  pilT Neisseria gonorrhoeae MS11

64.242

95.652

0.614
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.988

94.783

0.493
  pilU Vibrio cholerae strain A1552

42.216

96.812

0.409
  pilU Pseudomonas stutzeri DSM 10701

40.828

97.971

0.4
  pilU Acinetobacter baylyi ADP1

40.625

92.754

0.377

Multiple sequence alignment    



References

[1] Hannah Q Hughes et al. (2022) The PilT retraction ATPase promotes both extension and retraction of the MSHA type IVa pilus in Vibrio cholerae. PLoS Genetics 18(12):e1010561. [PMID: 36542674]
[2] David W Adams et al. (2019) DNA-uptake pili of Vibrio cholerae are required for chitin colonization and capable of kin recognition via sequence-specific self-interaction. Nature Microbiology 4(9):1545-1557. [PMID: 31182799]
[3] Patrick Seitz et al. (2013) DNA-uptake machinery of naturally competent Vibrio cholerae. Proceedings of The National Academy of Sciences of The United States of America 110(44):17987-92. [PMID: 24127573]