Detailed information    

experimental Experimentally validated

Overview

Name   pilT   Type   Machinery gene
Locus tag   GCO85_RS10305 Genome accession   NZ_CP048618
Coordinates   2285782..2286816 (-) Length   344 a.a.
NCBI ID   WP_010947729.1    Uniprot ID   Q5ZTZ5
Organism   Legionella pneumophila strain ERS1305867     
Function   assembly of type IV pilus   
DNA binding and uptake

Function

Mutation of this gene caused a decrease in transformation.


Genomic Context

Location: 2280782..2291816
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GCO85_RS10285 (GCO85_10295) rpoZ 2282689..2282892 (-) 204 WP_011214258.1 DNA-directed RNA polymerase subunit omega -
  GCO85_RS10290 (GCO85_10300) gmk 2282943..2283572 (-) 630 WP_027220542.1 guanylate kinase -
  GCO85_RS10295 (GCO85_10305) - 2283578..2284444 (-) 867 WP_027220543.1 YicC/YloC family endoribonuclease -
  GCO85_RS10300 (GCO85_10310) rph 2284746..2285453 (+) 708 WP_010947728.1 ribonuclease PH -
  GCO85_RS10305 (GCO85_10315) pilT 2285782..2286816 (-) 1035 WP_010947729.1 type IV pilus twitching motility protein PilT Machinery gene
  GCO85_RS10310 (GCO85_10320) - 2286980..2287672 (+) 693 WP_027225477.1 YggS family pyridoxal phosphate-dependent enzyme -
  GCO85_RS10315 (GCO85_10325) proC 2287672..2288460 (+) 789 WP_061637213.1 pyrroline-5-carboxylate reductase -
  GCO85_RS10320 (GCO85_10330) - 2288576..2288839 (-) 264 WP_042755224.1 GIY-YIG nuclease family protein -
  GCO85_RS10325 (GCO85_10335) - 2288839..2289210 (-) 372 Protein_2031 IS4 family transposase -
  GCO85_RS10330 (GCO85_10340) - 2289669..2290244 (+) 576 WP_027225573.1 YggT family protein -
  GCO85_RS10335 (GCO85_10345) - 2290304..2290906 (+) 603 WP_014842061.1 DUF2845 domain-containing protein -
  GCO85_RS10340 (GCO85_10350) - 2290920..2291201 (+) 282 WP_010947734.1 hypothetical protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38386.27 Da        Isoelectric Point: 7.0290

>NTDB_id=1259 GCO85_RS10305 WP_010947729.1 2285782..2286816(-) (pilT) [Legionella pneumophila strain ERS1305867]
MDIAELLAFSVKNKSSDLHISAGMPPMIRVDGDLRKINLPPLEHKDVIKIIYDIMNDRQRKEYEEFFETDFSFEIANLAR
FRVNAFNQSRGAAAVFRTIPSEILSMEDLGLPPIFKEMASFSRGLVLVTGPTGSGKSTTLAAVIDYINSSRYEHILTVED
PIEFVHQSKKCLVNQREVHKDTLSFNAALRSALREDPDIILVGELRDLETIRLAMTAAETGHLVFGTLHTNSATKTINRI
IDVFPAEEKSMVRSMLSESLQAVVAQSLLKKNKGGRVAALEIMMCTGAIRNLIREDKIAQMYSSIQTGQAKGMQTLDQHL
TELVNKNIISKHTAHEAALNKSLF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=1259 GCO85_RS10305 WP_010947729.1 2285782..2286816(-) (pilT) [Legionella pneumophila strain ERS1305867]
ATGGATATCGCGGAATTGTTGGCATTTTCAGTTAAAAACAAGTCATCAGATTTGCATATTTCAGCTGGCATGCCTCCTAT
GATTCGTGTTGATGGTGATTTACGCAAAATTAATTTACCTCCATTAGAGCATAAAGACGTCATAAAGATTATTTATGACA
TAATGAATGACAGACAAAGAAAAGAATATGAAGAATTTTTTGAAACAGATTTTTCCTTTGAAATAGCTAATCTGGCACGG
TTCAGGGTTAATGCTTTCAATCAATCACGCGGCGCCGCTGCTGTTTTCAGAACTATTCCCTCTGAAATTCTTAGCATGGA
AGATTTAGGATTACCTCCAATTTTTAAAGAAATGGCCAGTTTTTCCAGAGGGCTGGTTCTGGTTACTGGCCCAACTGGAT
CAGGAAAAAGTACCACGCTTGCAGCAGTCATCGATTATATTAATTCAAGTCGTTATGAGCATATTTTGACAGTTGAAGAT
CCCATTGAATTTGTTCATCAAAGCAAAAAATGTCTGGTCAATCAACGTGAAGTGCACAAAGACACATTGAGCTTTAATGC
CGCTTTGAGATCAGCGCTACGTGAAGATCCTGATATTATATTGGTGGGTGAATTACGTGATTTGGAAACTATACGCTTGG
CAATGACCGCAGCAGAAACCGGGCATTTGGTTTTTGGAACATTGCATACCAATTCTGCAACAAAAACAATAAACAGGATA
ATTGATGTATTTCCTGCTGAAGAAAAATCGATGGTACGCTCTATGCTTTCTGAATCATTACAAGCTGTCGTTGCTCAAAG
TTTATTAAAAAAGAATAAAGGTGGTCGGGTTGCGGCGCTTGAAATTATGATGTGTACCGGGGCTATTCGTAATTTAATTC
GTGAGGATAAGATAGCGCAAATGTATTCGTCAATTCAAACTGGTCAAGCCAAAGGAATGCAAACATTGGATCAACATTTG
ACTGAATTGGTTAATAAAAATATTATTTCGAAACACACTGCTCATGAGGCTGCCCTGAACAAGTCATTATTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q5ZTZ5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Legionella pneumophila strain Lp02

100

100

1
  pilT Acinetobacter baumannii strain A118

75.581

100

0.756
  pilT Acinetobacter baumannii D1279779

75.581

100

0.756
  pilT Acinetobacter nosocomialis M2

75.581

100

0.756
  pilT Acinetobacter baylyi ADP1

73.256

100

0.733
  pilT Pseudomonas aeruginosa PAK

74.556

98.256

0.733
  pilT Pseudomonas stutzeri DSM 10701

74.26

98.256

0.73
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.477

95.64

0.645
  pilT Vibrio cholerae strain A1552

67.477

95.64

0.645
  pilT Neisseria meningitidis 8013

64.809

99.128

0.642
  pilT Neisseria gonorrhoeae MS11

64.516

99.128

0.64
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.275

100

0.494
  pilU Vibrio cholerae strain A1552

42.773

98.547

0.422
  pilU Pseudomonas stutzeri DSM 10701

41.791

97.384

0.407
  pilU Acinetobacter baylyi ADP1

40.361

96.512

0.39

Multiple sequence alignment    



References

[1] Léo Hardy et al. (2021) Transposon Insertion Sequencing in a Clinical Isolate of Legionella pneumophila Identifies Essential Genes and Determinants of Natural Transformation. Journal of Bacteriology 203(3):e00548-20. [PMID: 33168636]