Detailed information    

experimental Experimentally validated

Overview

Name   pilU   Type   Machinery gene
Locus tag   PSJM300_01605 Genome accession   CP003725
Coordinates   344278..345423 (+) Length   381 a.a.
NCBI ID   AFN76401.1    Uniprot ID   -
Organism   Pseudomonas stutzeri DSM 10701     
Function   assembly of type IV pilus   
DNA binding and uptake

Function

Insertional inactivation of pilU did not affect piliation, twitching motility, or PO4 infection but reduced transformation to about 10%.


Genomic Context

Location: 339278..350423
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSJM300_01585 - 340977..341567 (-) 591 AFN76397.1 YGGT family protein -
  PSJM300_01590 - 341564..342391 (-) 828 AFN76398.1 pyrroline-5-carboxylate reductase -
  PSJM300_01595 - 342408..343118 (-) 711 AFN76399.1 alanine racemase domain-containing protein -
  PSJM300_01600 pilT 343186..344220 (+) 1035 AFN76400.1 twitching motility protein PilT Machinery gene
  PSJM300_01605 pilU 344278..345423 (+) 1146 AFN76401.1 twitching motility protein PilU Machinery gene
  PSJM300_01610 - 345477..346313 (-) 837 AFN76402.1 leucyl-tRNA synthetase -
  PSJM300_01615 - 346313..347524 (-) 1212 AFN76403.1 elongator protein -
  PSJM300_01620 - 347676..348074 (+) 399 AFN76404.1 hypothetical protein -
  PSJM300_01625 pyrC 348119..349390 (-) 1272 AFN76405.1 dihydroorotase -
  PSJM300_01630 pyrB 349390..350406 (-) 1017 AFN76406.1 aspartate carbamoyltransferase catalytic subunit -

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 42417.64 Da        Isoelectric Point: 6.5773

>NTDB_id=1203 PSJM300_01605 AFN76401.1 344278..345423(+) (pilU) [Pseudomonas stutzeri DSM 10701]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGKILPVNKTPMSPEMTRETVHGVMNEQQRREFTENHECNFAISARGIGR
FRVSAFYQRNLAGMVLRRIETNIPTIEDLKLPDVLKKLALTKRGLVLFVGATGTGKSTSLAAMIGYRNKNSSGHIISIED
PIEFIHQHQNCIVTQREVGIDTDSFEVALKNTLRQAPDVILIGEVRTRETMDHAVAFAETGHLCLATLHANNANQALDRI
INFFPADRQNQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLINTPLAADLIRKGEVHELKGLMKRSTDLGMQTFDQA
LYNLYVQGEITYEDALLHADSSNDLRLMIKLGSETDGEHLTSVSQGLTLEMSDDDPGRSFR

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=1203 PSJM300_01605 AFN76401.1 344278..345423(+) (pilU) [Pseudomonas stutzeri DSM 10701]
ATGGAATTCGAGAAACTGTTGCGCCTGATGGTGGAAAAAGGCGGCTCCGACCTGTTCATTACTGCGGGCGTGCCACCCTC
GATGAAGGTCAATGGCAAGATCCTGCCGGTGAACAAGACGCCGATGTCGCCGGAGATGACCCGCGAAACCGTGCATGGAG
TGATGAACGAGCAGCAGCGCCGCGAGTTCACCGAGAACCACGAGTGCAACTTCGCCATCAGCGCCCGTGGCATCGGCCGC
TTCCGTGTCAGCGCCTTCTATCAGCGCAACCTGGCGGGCATGGTGCTGCGGCGGATCGAAACCAATATCCCGACGATAGA
AGACCTCAAGCTGCCCGACGTGCTGAAGAAGCTGGCCCTGACCAAGCGTGGTCTGGTGCTCTTCGTCGGCGCCACCGGTA
CCGGCAAGTCCACCTCGCTGGCGGCAATGATCGGCTATCGGAACAAGAACTCCAGCGGCCACATCATCTCCATCGAAGAC
CCCATCGAATTCATCCATCAGCACCAGAACTGCATCGTCACCCAGCGCGAAGTGGGCATCGATACCGACTCGTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCCTGATCGGTGAGGTGCGTACCCGCGAAACCATGGACCATG
CCGTGGCCTTCGCCGAAACCGGCCACCTGTGCCTGGCCACGCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCCGCCGACCGGCAGAACCAGGTATGGATGGACCTGTCGCTCAACCTCAAGGCCATCGTCGCGCAGCA
GCTGGTGCCGACCCCGGATGGCAAGGGCCGTCGTGCGGTCATCGAGGTGCTGATCAACACGCCACTGGCGGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTCAAGGGCCTGATGAAGCGCTCCACCGATCTGGGCATGCAGACCTTCGACCAGGCG
CTGTACAACCTCTATGTACAGGGCGAAATCACTTACGAGGACGCCTTGCTGCACGCCGACTCGTCCAACGACCTGCGCCT
GATGATCAAGCTGGGCTCGGAAACCGATGGCGAGCATCTCACCAGTGTCTCCCAGGGCCTGACCCTGGAAATGAGCGATG
ACGACCCGGGGCGCAGTTTCCGCTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(113-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

65.266

93.701

0.612
  pilU Vibrio cholerae strain A1552

58.857

95.109

0.56
  pilT Legionella pneumophila strain ERS1305867

41.791

97.384

0.407
  pilT Legionella pneumophila strain Lp02

41.791

97.384

0.407
  pilT Neisseria meningitidis 8013

40.29

99.424

0.401
  pilT Neisseria gonorrhoeae MS11

40.29

99.424

0.401
  pilT Vibrio cholerae strain A1552

40.828

97.971

0.4
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.828

97.971

0.4
  pilT Pseudomonas aeruginosa PAK

40.896

97.384

0.398
  pilT Pseudomonas stutzeri DSM 10701

40.896

97.384

0.398
  pilT Acinetobacter baumannii D1279779

40.597

97.101

0.394
  pilT Acinetobacter nosocomialis M2

40.597

97.101

0.394
  pilT Acinetobacter baumannii strain A118

40.597

97.101

0.394
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.658

88.976

0.388
  pilT Acinetobacter baylyi ADP1

39.701

97.384

0.387

Multiple sequence alignment    



References

[1] S Graupner et al. (2001) Requirement of novel competence genes pilT and pilU of Pseudomonas stutzeri for natural transformation and suppression of pilT deficiency by a hexahistidine tag on the type IV pilus protein PilAI. Journal of Bacteriology 183(16):4694-701. [PMID: 11466271]