Detailed information NTG0362214

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: FDQ49_RS05340
• Genome accession: NZ_CP040105
• Coordinates: 1152644..1153762 (+)
• Length: 372 a.a.
• NCBI ID: WP_002052019.1
• Uniprot ID: A0A1V0YUM2
• Organism: Acinetobacter nosocomialis M2
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1147644..1158762

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FDQ49_RS05325 (FDQ49_05325)	-	1149810..1150652 (-)	843	WP_022575104.1	undecaprenyl-diphosphate phosphatase	-
FDQ49_RS05330 (FDQ49_05330)	-	1150760..1151452 (-)	693	WP_022575105.1	YggS family pyridoxal phosphate-dependent enzyme	-
FDQ49_RS05335 (FDQ49_05335)	pilT	1151579..1152616 (+)	1038	WP_006581387.1	type IV pilus twitching motility protein PilT	Machinery gene
FDQ49_RS05340 (FDQ49_05340)	pilU	1152644..1153762 (+)	1119	WP_002052019.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
FDQ49_RS05345 (FDQ49_05345)	fur	1153832..1154269 (-)	438	WP_002052029.1	ferric iron uptake transcriptional regulator	-
FDQ49_RS05350 (FDQ49_05350)	bamE	1154381..1154779 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
FDQ49_RS05355 (FDQ49_05355)	-	1154835..1155158 (-)	324	WP_002052062.1	RnfH family protein	-
FDQ49_RS05360 (FDQ49_05360)	-	1155158..1156225 (-)	1068	WP_022575106.1	membrane protein	-
FDQ49_RS05370 (FDQ49_05370)	-	1156378..1156833 (-)	456	WP_000782976.1	bacteriohemerythrin	-
FDQ49_RS05375 (FDQ49_05375)	-	1157086..1157934 (-)	849	WP_022575107.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41980.18 Da
• Isoelectric Point: 6.6944

>NTDB_id=362214 FDQ49_RS05340 WP_002052019.1 1152644..1153762(+) (pilU) [Acinetobacter nosocomialis M2]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNRDKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPTVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLANEGPDQITDEKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=362214 FDQ49_RS05340 WP_002052019.1 1152644..1153762(+) (pilU) [Acinetobacter nosocomialis M2]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCATCCGATCTGTTTATTACAGATGGTGTTGCACCATC
GATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAATAGCCTTTCAGGTGAAGTAATTGGTCAACTCTTACATTCCA
TCATGAGTGAAAAACAACGTAAAGAATTTGCAGAGACTCGTGAATGTAACTTTGCCATCATGAACCGCGATAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAACGTGACATGCCGGGTATGGTACTGCGTCGAATTGAAACGAAAATTCCTTCAAT
TGATGACTTACAGTTGCCGACTGTGCTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTCGTAGGTGCAACAG
GTACAGGTAAATCGACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAAGGCCATATCATTACCATTGAG
GACCCGATTGAGTTTATTCACGAACATGCAGGCTGCATTATTACTCAACGTGAAGTCGGTATTGATACGGACTCTTTCGA
AATTGCTTTAAAAAATACATTACGACAAGCACCCGATGTCATCTTAATTGGTGAGATTCGTTCTCGTGAAGTCATGGACT
ACGCAATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCTACAATGCACGCCAACAACGCCAACCAAGCACTCGACCGT
ATTATTCACTTCTTTGAAAGTGACCGTCATAGCCAGCTCTACATGGACTTGTCTCTTAACTTAAAAGCCATGATTGCACA
ACAGCTTATTCCAACACCAGATGGTAATTCACGCCGTGCAGCGATTGAGATCTTAATTAACTCACCATTAATTTCAGACT
ATATCCGTAAAGGTGAAATTCATGAAATTAAGGACTTGATGAAACGCTCACGTGAGCTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATACAAAGCCGGCCAAATTACTTATAAAGATGCGCTTAAACATGCCGACTCACCAAACGATTTACG
TTTAACGATTAAACTTGCAAATGAAGGTCCTGATCAAATAACAGACGAAAAACAGCATTTAACTTTTGATCGTCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (32-270)

3D structure

• AlphaFold DB: A0A1V0YUM2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.132	100	0.763
pilU	Pseudomonas stutzeri DSM 10701	68.464	99.731	0.683
pilU	Vibrio cholerae strain A1552	53.514	99.462	0.532
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.254	95.43	0.403