Detailed information NTG1128551

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PAKAF_RS02000
• Genome accession: NZ_LR657304
• Coordinates: 426271..427419 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa PAK
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 421271..432419

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PAKAF_RS01975	-	422422..422718 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
PAKAF_RS01980 (PAKAF_00396)	-	422715..423308 (-)	594	WP_003155373.1	YggT family protein	-
PAKAF_RS01985 (PAKAF_00397)	proC	423319..424140 (-)	822	WP_003142635.1	pyrroline-5-carboxylate reductase	-
PAKAF_RS01990 (PAKAF_00398)	-	424152..424844 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
PAKAF_RS01995 (PAKAF_00399)	pilT	425059..426093 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
PAKAF_RS02000 (PAKAF_00400)	pilU	426271..427419 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
PAKAF_RS02005 (PAKAF_00401)	czcD	427427..428326 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
PAKAF_RS02010 (PAKAF_00402)	-	428480..428884 (+)	405	WP_003084560.1	NINE protein	-
PAKAF_RS02015 (PAKAF_00403)	-	429127..430500 (+)	1374	WP_003084562.1	pyridoxal-phosphate dependent enzyme	-
PAKAF_RS02020 (PAKAF_00404)	-	430497..431681 (+)	1185	WP_003100953.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=1128551 PAKAF_RS02000 WP_003100959.1 426271..427419(+) (pilU) [Pseudomonas aeruginosa PAK]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=1128551 PAKAF_RS02000 WP_003100959.1 426271..427419(+) (pilU) [Pseudomonas aeruginosa PAK]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACTTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGTCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382