Detailed information    

experimental Experimentally validated

Overview

Name   dprA   Type   Machinery gene
Locus tag   H0N27_RS16615 Genome accession   NZ_CP059039
Coordinates   3555854..3556984 (+) Length   376 a.a.
NCBI ID   WP_168727007.1    Uniprot ID   -
Organism   Acinetobacter baumannii strain A118     
Function   ssDNA binding; loading RecA onto ssDNA   
DNA processing

Function

Mutant studies with A. baumannii A118 confirmed that pilA, pilQ (secretin, comQ homologue), pilT (retraction ATPase), comEA (DNA-binding protein), comF (pilotin) and dprA (DNA protecting protein) are required for natural transformation


Genomic Context

Location: 3550854..3561984
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H0N27_RS16590 (H0N27_16545) - 3551115..3551321 (-) 207 WP_000182542.1 hypothetical protein -
  H0N27_RS16595 (H0N27_16550) - 3551375..3553489 (-) 2115 WP_168727010.1 TonB-dependent copper receptor -
  H0N27_RS16600 (H0N27_16555) - 3553561..3553989 (-) 429 WP_168727009.1 DUF2946 family protein -
  H0N27_RS16605 (H0N27_16560) def 3554026..3554556 (-) 531 WP_001196539.1 peptide deformylase -
  H0N27_RS16610 (H0N27_16565) tsaP 3554679..3555833 (+) 1155 WP_168727008.1 LysM peptidoglycan-binding domain-containing protein Machinery gene
  H0N27_RS16615 (H0N27_16570) dprA 3555854..3556984 (+) 1131 WP_168727007.1 DNA-processing protein DprA Machinery gene
  H0N27_RS16620 (H0N27_16575) - 3557035..3557604 (+) 570 WP_098715214.1 L-threonylcarbamoyladenylate synthase -
  H0N27_RS16625 (H0N27_16580) - 3557996..3558916 (-) 921 WP_001994180.1 DMT family transporter -
  H0N27_RS16630 (H0N27_16585) - 3559022..3559672 (-) 651 WP_086227614.1 TetR/AcrR family transcriptional regulator -
  H0N27_RS16635 (H0N27_16590) - 3559809..3561023 (+) 1215 WP_000372838.1 MFS transporter -
  H0N27_RS16640 (H0N27_16595) - 3561045..3561809 (+) 765 WP_000786954.1 AraC family transcriptional regulator -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  hns dprA negative effect
  hns pilQ negative effect
  hns pilT negative effect
  hns comA/comEC negative effect
  hns comF negative effect
  hns pilA negative effect
  pilR pilA positive effect
  hns comEA negative effect
  pilS pilR positive effect

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41935.49 Da        Isoelectric Point: 6.6297

>NTDB_id=1043 H0N27_RS16615 WP_168727007.1 3555854..3556984(+) (dprA) [Acinetobacter baumannii strain A118]
MLTQLSSHQYHTLKVWYLVQHSLVSFKKIIDYFGNCEKATQPDGLAKWSSLGLHANHLKRVNEFQTPQGQAQFEQLVQQV
RQHTDFILTPDDSGYPTQLLPYTDHPPIIFGKGQAQALLQPQIAIVGSRKPSPHGRQVAYDFAYYLSEKGFFISSGLAYG
IDEAAHQGASTHQRTIAVTGTGLDTTYPAQNKKLAEHILAKNGAIITEFLPGTPPLQQHFPRRNRIVSGLSLGVLVVEAT
LKSGSLITANKAAEQGKTVFAIPGHIYSEFHQGCHQLIREGAILIDHPEQIIEDLALPTQWQSQQQNQTEETNTNTPEIP
EHLIDLYQSLDWVGQNIDQLVVHHNIPVSELTSSLMELELLGLCMQQSGLYLRCRS

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=1043 H0N27_RS16615 WP_168727007.1 3555854..3556984(+) (dprA) [Acinetobacter baumannii strain A118]
ATGCTCACTCAACTTTCTTCACACCAATACCACACACTTAAAGTGTGGTATTTGGTGCAGCACTCTTTAGTCAGCTTTAA
AAAAATTATTGATTATTTTGGAAATTGTGAAAAAGCCACTCAACCTGATGGTTTAGCAAAGTGGTCCTCTCTGGGGCTAC
ATGCTAATCACTTAAAGCGTGTAAACGAATTTCAAACGCCCCAAGGTCAAGCGCAGTTTGAACAACTTGTACAACAAGTT
CGTCAGCATACGGATTTTATTTTAACGCCAGATGACTCTGGCTATCCCACTCAATTACTTCCTTATACAGATCACCCACC
TATTATTTTTGGGAAAGGTCAAGCGCAAGCTTTATTGCAACCTCAAATTGCGATTGTCGGTAGCCGAAAGCCAAGCCCAC
ATGGACGACAGGTCGCTTATGATTTTGCCTATTACTTAAGTGAAAAGGGCTTTTTCATTAGTAGCGGTCTGGCTTATGGC
ATTGATGAAGCCGCTCATCAAGGTGCATCTACTCATCAACGTACCATTGCAGTGACGGGTACGGGCTTAGACACTACTTA
TCCAGCTCAAAATAAAAAACTTGCAGAACATATTTTGGCTAAAAACGGCGCCATTATTACTGAGTTTTTACCGGGTACAC
CACCATTACAACAGCATTTCCCACGTAGAAACCGAATTGTCAGCGGTTTGAGTTTAGGCGTTTTGGTGGTAGAAGCGACA
TTAAAAAGCGGCTCACTCATCACGGCCAATAAAGCTGCTGAACAAGGTAAAACCGTATTCGCGATTCCGGGCCATATTTA
TAGCGAGTTTCATCAAGGATGCCATCAACTTATCCGTGAAGGTGCAATTTTAATCGACCATCCTGAACAGATTATTGAGG
ATCTTGCTTTACCCACTCAATGGCAAAGTCAGCAACAAAATCAAACTGAAGAAACTAATACGAATACTCCAGAAATTCCT
GAGCATTTGATTGACCTTTATCAGTCTTTAGACTGGGTGGGACAAAATATTGACCAGCTTGTAGTTCACCATAATATTCC
AGTTTCTGAACTGACCTCTTCACTAATGGAACTAGAACTACTCGGTTTATGTATGCAACAATCAGGGCTGTATTTACGTT
GTCGTTCTTAG

Domains

Predicted by InterproScan.

DNA recombination-mediator protein A (DNA_processg_A)

(87-285)

DprA winged helix domain (DprA_WH)

(331-369)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Acinetobacter baumannii D1279779

98.138

100

0.981
  dprA Acinetobacter baylyi ADP1

70.079

100

0.71
  dprA Vibrio cholerae strain A1552

43.202

89.218

0.385

Multiple sequence alignment    



References

[1] Nina Vesel et al. (2021) Pilus Production in Acinetobacter baumannii Is Growth Phase Dependent and Essential for Natural Transformation. Journal of Bacteriology 203(8):e00034-21. [PMID: 33495250]
[2] Casin Le et al. (2021) Involvement of the Histone-Like Nucleoid Structuring Protein (H-NS) in Acinetobacter baumannii's Natural Transformation. Pathogens (Basel, Switzerland) 10(9):1083. [PMID: 34578115]