Detailed information    

experimental Experimentally validated

Overview

Name   pilQ   Type   Machinery gene
Locus tag   H0N27_RS01460 Genome accession   NZ_CP059039
Coordinates   315074..317239 (+) Length   721 a.a.
NCBI ID   WP_031993779.1    Uniprot ID   -
Organism   Acinetobacter baumannii strain A118     
Function   assembly of type IV pilus   
DNA binding and uptake

Function

Mutant studies with A. baumannii A118 confirmed that pilA, pilQ (secretin, comQ homologue), pilT (retraction ATPase), comEA (DNA-binding protein), comF (pilotin) and dprA (DNA protecting protein) are required for natural transformation


Genomic Context

Location: 310074..322239
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H0N27_RS01440 (H0N27_01440) pilM 312037..313095 (+) 1059 WP_002014066.1 pilus assembly protein PilM Machinery gene
  H0N27_RS01445 (H0N27_01445) pilN 313095..313736 (+) 642 WP_118978219.1 PilN domain-containing protein Machinery gene
  H0N27_RS01450 (H0N27_01450) pilO 313733..314473 (+) 741 WP_000076101.1 type 4a pilus biogenesis protein PilO Machinery gene
  H0N27_RS01455 (H0N27_01455) pilP 314484..315011 (+) 528 WP_000695065.1 pilus assembly protein PilP Machinery gene
  H0N27_RS01460 (H0N27_01460) pilQ 315074..317239 (+) 2166 WP_031993779.1 type IV pilus secretin PilQ Machinery gene
  H0N27_RS01465 (H0N27_01465) aroK 317251..317820 (+) 570 WP_000643712.1 shikimate kinase AroK -
  H0N27_RS01470 (H0N27_01470) aroB 317849..318931 (+) 1083 WP_168726692.1 3-dehydroquinate synthase -
  H0N27_RS01475 (H0N27_01475) - 318947..319822 (+) 876 WP_168726693.1 hypothetical protein -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  hns pilQ negative effect
  hns pilT negative effect
  hns comA/comEC negative effect
  hns comF negative effect
  hns pilA negative effect
  pilR pilA positive effect
  hns dprA negative effect
  hns comEA negative effect
  pilS pilR positive effect

Sequence

Protein


Download         Length: 721 a.a.        Molecular weight: 77921.31 Da        Isoelectric Point: 6.5404

>NTDB_id=1039 H0N27_RS01460 WP_031993779.1 315074..317239(+) (pilQ) [Acinetobacter baumannii strain A118]
MNHVFRQFSMGAVAIAIMQAASAQVSMTNIVPMQIAGQGTEIRVMFNGLPPQPQAYQLENPSRLILDFDKAQQGLKQSKI
SVATNEASSVDVTSDDQRSRLTVNLKDTGAFTTRVEGNTFILKINSAQTSNKPLPVVSAQPQGVSNIGFQRGSQGEGLVV
VDLLGSNTPVDVQQQGSKVVIRTIGTKIPTHLARRLNVNDFATPVSSIDAYNDKGVGVITIQSSGSYEYMAYQAENKLTI
SLKRPQDKNVTSLYKTPNYSGNKLSLDFQDIEVRRVLQLLADFTGINMVAADSVQGNITLRLKDVPWDQALDIILKTKNL
DKRRNGNVIWIAPVAELIKAEEEEAKAVAQSVKLAPLQTEYIQLKYAKAGDIMGLITQGSNNSNGLQHTSGGGTSTSTNL
NTGVDSLGNNVGSLLSPRGTITQDDRTNTLIINDTAQSIDQIRKMIDLLDVQVKQVMVEARIVRASTSFTKELGVKWGIL
SQGITNNNNLLVGGSETTLWNLREPKKDETTGGYKYTIERPDNLNVDLGVSNPAGSIAFGLISMSDFMLDLELSALQADG
YGEVISTPKVMTADKQTAKVATGQQVPYQSTTNSAAGSTATTSFKDALLSLNVTPSITPDGKIQMKLDISKDSVAGAAPN
GELILNKNNINTNVLVDNGETVILGGVFEQTTMNSQTKVPFFGDIPVVGRLFRKDVKSDDKQELLIFVTPRIVNDTLARN
H

Nucleotide


Download         Length: 2166 bp        

>NTDB_id=1039 H0N27_RS01460 WP_031993779.1 315074..317239(+) (pilQ) [Acinetobacter baumannii strain A118]
ATGAATCACGTATTCCGTCAGTTTTCTATGGGGGCTGTAGCTATTGCGATTATGCAGGCTGCATCTGCACAGGTCAGCAT
GACCAACATTGTACCAATGCAAATTGCAGGTCAGGGCACAGAAATTAGAGTCATGTTTAATGGCTTGCCACCACAACCAC
AAGCTTACCAACTTGAAAATCCTTCACGTTTAATCCTTGATTTTGATAAGGCACAGCAAGGTTTAAAACAGTCTAAAATT
AGTGTTGCAACTAATGAGGCTAGTTCTGTAGATGTTACTTCAGATGACCAGCGTTCACGTTTAACTGTAAACCTAAAAGA
TACTGGAGCATTTACAACACGAGTAGAAGGTAATACCTTTATTTTAAAAATTAATTCTGCTCAAACAAGTAATAAGCCGC
TACCTGTGGTCTCAGCACAACCACAAGGTGTTTCAAATATTGGCTTTCAACGTGGTAGTCAGGGAGAAGGTCTGGTTGTT
GTAGATCTTTTAGGAAGTAATACGCCTGTAGATGTTCAGCAGCAGGGTAGTAAAGTCGTTATAAGAACAATAGGTACAAA
AATCCCGACACATTTAGCACGTCGCTTAAATGTAAATGATTTTGCAACACCGGTTTCAAGTATCGATGCTTATAATGATA
AAGGGGTTGGTGTAATAACTATCCAGTCCTCTGGTAGCTATGAATATATGGCCTATCAGGCAGAGAATAAGCTCACGATC
AGTTTGAAACGTCCCCAAGATAAAAATGTTACCTCTTTATATAAAACGCCGAATTATTCGGGAAATAAACTTTCATTAGA
CTTCCAAGATATTGAAGTACGCCGTGTGTTGCAATTACTTGCTGATTTTACAGGCATCAATATGGTAGCTGCCGATAGTG
TACAAGGTAATATCACACTACGTTTAAAAGATGTACCTTGGGATCAGGCTCTTGATATTATTTTAAAAACTAAAAATCTT
GATAAACGCCGTAACGGCAATGTGATTTGGATTGCTCCAGTTGCAGAATTGATTAAAGCGGAAGAAGAAGAAGCGAAAGC
TGTTGCACAAAGTGTCAAACTTGCACCCTTACAGACTGAGTATATTCAACTGAAGTATGCTAAAGCTGGCGATATTATGG
GTCTCATTACACAAGGCTCAAATAATAGTAATGGCTTACAACATACTTCTGGTGGAGGGACCTCAACTTCAACCAATTTA
AATACTGGAGTTGATAGTCTAGGCAATAATGTGGGTAGTTTGCTTAGCCCGCGTGGCACTATTACTCAAGATGACCGAAC
TAATACTCTTATTATTAACGATACTGCTCAGTCAATTGATCAAATTCGTAAAATGATTGACTTGCTTGATGTGCAAGTTA
AACAGGTTATGGTTGAAGCACGTATTGTGAGAGCCTCTACGTCATTTACCAAAGAACTTGGTGTGAAGTGGGGCATTCTG
TCGCAAGGTATTACCAATAACAACAATCTGCTGGTTGGCGGTAGTGAAACAACTTTATGGAATTTACGCGAACCTAAAAA
AGATGAAACTACAGGTGGGTATAAATATACGATTGAGCGCCCAGATAACTTAAATGTGGATTTAGGAGTTTCTAATCCTG
CAGGAAGTATTGCTTTTGGCTTGATTAGTATGTCTGACTTTATGTTAGATCTTGAATTGTCTGCTTTACAAGCGGATGGT
TATGGTGAGGTTATTTCTACCCCTAAAGTCATGACTGCCGATAAACAAACAGCGAAAGTTGCAACTGGTCAACAAGTTCC
ATATCAATCTACAACTAACTCTGCAGCAGGTTCAACTGCTACAACATCTTTTAAAGATGCTTTGCTGAGTTTAAATGTGA
CACCAAGTATTACACCTGATGGCAAGATTCAAATGAAGTTGGATATTTCTAAGGATTCTGTAGCGGGTGCGGCTCCAAAT
GGAGAGTTGATTTTAAATAAAAATAATATCAACACTAATGTATTGGTAGATAATGGCGAAACTGTCATTTTAGGTGGTGT
TTTTGAGCAAACAACTATGAATTCACAAACCAAAGTTCCTTTCTTTGGTGATATTCCTGTTGTTGGGCGTTTATTTAGAA
AAGATGTAAAGTCTGATGATAAACAAGAGTTGCTGATTTTCGTTACCCCACGAATTGTTAATGACACTTTAGCGAGAAAT
CATTAA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(39-122)

Secretin and TonB N terminus short domain (STN)

(291-333)

Bacterial type II/III secretion system short domain (Secretin_N)

(360-455)

Bacterial type II and III secretion system protein (Secretin)

(555-713)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Acinetobacter baumannii D1279779

99.861

100

0.999
  comQ Acinetobacter baylyi ADP1

75.103

100

0.77
  pilQ Pseudomonas aeruginosa PAK

40.699

100

0.408
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

37.819

100

0.389
  pilQ Vibrio cholerae strain A1552

37.819

100

0.389
  pilQ Vibrio campbellii strain DS40M4

37.521

100

0.386
  pilQ Legionella pneumophila strain Lp02

35.095

100

0.371

Multiple sequence alignment    



References

[1] Nina Vesel et al. (2021) Pilus Production in Acinetobacter baumannii Is Growth Phase Dependent and Essential for Natural Transformation. Journal of Bacteriology 203(8):e00034-21. [PMID: 33495250]
[2] Casin Le et al. (2021) Involvement of the Histone-Like Nucleoid Structuring Protein (H-NS) in Acinetobacter baumannii's Natural Transformation. Pathogens (Basel, Switzerland) 10(9):1083. [PMID: 34578115]