Detailed information NTG0001284

Experimentally validated

Overview

• Name: pilQ
• Type: Machinery gene
• Locus tag: ACE17W_RS12370
• Genome accession: NZ_CP169562
• Coordinates: 2762153..2764249 (+)
• Length: 698 a.a.
• NCBI ID: WP_010946666.1
• Uniprot ID: Q5ZX02
• Organism: Legionella pneumophila strain Lp02
• Function: assembly of type IV pilus DNA binding and uptake

Function

Deletion of comEC, encoding the putative IM DNA channel, pilQ, encoding the OM secretin, and pilT, encoding the retraction ATPase, resulted in undetectable levels of natural transformation in our assay.

Genomic Context

Location: 2757153..2769249

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACE17W_RS12345 (ACE17W_12345)	ravI	2757909..2758919 (-)	1011	WP_010946661.1	Dot/Icm T4SS effector RavI	-
ACE17W_RS12350 (ACE17W_12350)	pilM	2759336..2760400 (+)	1065	WP_010946662.1	type IV pilus assembly protein PilM	Machinery gene
ACE17W_RS12355 (ACE17W_12355)	pilN	2760404..2760952 (+)	549	WP_010946663.1	PilN domain-containing protein	Machinery gene
ACE17W_RS12360 (ACE17W_12360)	pilO	2760966..2761565 (+)	600	WP_014841277.1	type 4a pilus biogenesis protein PilO	Machinery gene
ACE17W_RS12365 (ACE17W_12365)	pilP	2761562..2762149 (+)	588	WP_010946665.1	pilus assembly protein PilP	Machinery gene
ACE17W_RS12370 (ACE17W_12370)	pilQ	2762153..2764249 (+)	2097	WP_010946666.1	type IV pilus secretin PilQ	Machinery gene
ACE17W_RS12375 (ACE17W_12375)	aroK	2764695..2765222 (+)	528	WP_010946667.1	shikimate kinase AroK	-
ACE17W_RS12380 (ACE17W_12380)	aroB	2765209..2766318 (+)	1110	WP_010946668.1	3-dehydroquinate synthase	-
ACE17W_RS12385 (ACE17W_12385)	-	2766315..2767766 (+)	1452	WP_010946669.1	SPOR domain-containing protein	-
ACE17W_RS12390 (ACE17W_12390)	-	2767875..2768306 (+)	432	WP_010946670.1	universal stress protein	-

Sequence

Protein

• Length: 698 a.a.
• Molecular weight: 77487.79 Da
• Isoelectric Point: 9.5345

>NTDB_id=1284 ACE17W_RS12370 WP_010946666.1 2762153..2764249(+) (pilQ) [Legionella pneumophila strain Lp02]
MRKIIAILILISASLSMAYSKDNSLISVKVLPLPENRVRIDFQFSKPLKQLPASFITQKPPRLVLDFISTDMQIPSDQKT
KTIQLGSLHSYNIVAVGDRVRAILELDRSVSYSGSTAGSIYSLVLNGKSNDLFENSKEVFITNQVVNAKHEIKRIDFRGI
EKQGGRVLIDVSDTGIPIDVTQVGKEIVVNFLNTKIPLNLMKRYDVSDFHSPVQIITMQQEGKKVRMTILSKGDYGHFVY
QVNKQFMVDVFPLTAEEIRQAKLKKQVFTGKRISLNFQNISIRAVLQLLADFTGINMVVSDKVQGDITLRLNDIPWDQAL
DIILTTQGLDKRRKGNVMLIDTKASMDKMEEEQLKSQQTIEKLEPIRSDLIQINYAKAADIAVLIKDKQNSLLSDRGKIS
VDARTNTIWIQDTGTKIEEVRELIKQLDIPVKQVLIEARIVEVTKDFSQDIGIRWGVSRPTHLSGTLEGANELARGTAPA
NVVPLERRLNLDLAAAPLTGATPASVGIALAKLNDNILLDLELSALESEGRAELISSPRLITTNQQAAVIESGEEIPYQE
ATSSGATAVAFKKAVLSLKVTPQITPDGKILMDLQINQDTPSPQTFLGVPAIITKEIQTNVLVNNGQTIVLGGIYKQDKN
KVINRIPFFGELPVVGILFSNRQITLKNEELLIFITPRIITNALSITTIEGREKDVYK

Nucleotide

• Length: 2097 bp

>NTDB_id=1284 ACE17W_RS12370 WP_010946666.1 2762153..2764249(+) (pilQ) [Legionella pneumophila strain Lp02]
GTGAGAAAAATTATCGCGATTTTGATATTAATTAGTGCCAGTTTGAGTATGGCATATTCTAAAGATAATTCGCTCATTTC
AGTAAAAGTATTGCCATTGCCCGAAAACAGAGTGCGTATTGATTTTCAATTTTCCAAGCCCTTAAAGCAGTTGCCTGCAA
GCTTTATTACTCAAAAGCCGCCCCGTTTGGTGTTAGATTTTATCAGCACTGATATGCAAATTCCTTCGGATCAGAAAACT
AAAACTATTCAATTAGGCTCATTACACAGTTATAACATTGTTGCTGTTGGTGATAGAGTAAGAGCCATATTGGAACTGGA
TCGTTCAGTATCCTATTCTGGCAGCACGGCGGGTTCCATTTATAGCCTTGTCCTGAATGGTAAAAGCAATGATTTATTTG
AAAATAGCAAGGAGGTATTCATAACGAACCAGGTTGTGAACGCCAAGCACGAAATTAAAAGAATTGATTTTCGTGGTATA
GAAAAGCAGGGTGGTAGAGTCTTAATTGACGTTTCTGATACGGGTATTCCAATTGATGTAACTCAAGTTGGGAAAGAAAT
AGTCGTTAATTTCCTGAATACCAAGATCCCTTTGAATTTAATGAAACGGTATGATGTTTCTGATTTTCACAGCCCTGTGC
AGATTATTACGATGCAACAGGAAGGCAAGAAGGTTCGCATGACCATTCTCAGCAAAGGGGATTATGGACATTTTGTGTAT
CAGGTCAATAAACAATTTATGGTTGATGTCTTTCCTTTAACTGCGGAAGAAATTCGTCAGGCGAAATTAAAGAAACAGGT
ATTTACCGGTAAACGTATTTCTTTGAATTTCCAGAATATTAGTATTCGGGCTGTACTTCAATTGCTGGCAGATTTTACTG
GAATCAATATGGTTGTCAGCGACAAAGTACAAGGAGATATTACGCTTAGATTAAATGATATCCCCTGGGATCAGGCTCTG
GATATCATTTTAACCACACAAGGGCTAGATAAGCGCAGAAAAGGCAACGTCATGTTGATAGATACCAAGGCCTCCATGGA
TAAAATGGAGGAAGAACAACTCAAAAGTCAACAGACAATTGAAAAACTTGAGCCTATCCGTTCTGATTTAATACAGATTA
ATTATGCAAAGGCAGCAGATATTGCTGTTTTGATCAAGGATAAGCAAAATTCCCTATTATCAGACAGAGGAAAAATAAGT
GTTGATGCCAGAACGAATACAATCTGGATTCAGGATACTGGCACCAAAATTGAGGAAGTAAGGGAACTGATAAAGCAATT
GGATATCCCTGTTAAGCAAGTGCTTATTGAGGCAAGAATTGTTGAAGTAACTAAAGATTTTAGCCAGGATATTGGGATAC
GCTGGGGGGTATCAAGACCAACCCACTTAAGTGGTACACTGGAAGGTGCTAACGAGTTAGCGAGAGGCACAGCACCAGCC
AATGTTGTCCCCTTGGAAAGAAGGTTAAATCTTGATTTGGCAGCTGCTCCTTTAACTGGAGCTACACCAGCCTCGGTAGG
TATTGCGCTAGCTAAACTTAATGATAACATTCTGCTTGATCTGGAATTGTCTGCTTTGGAAAGCGAAGGGCGTGCTGAAT
TAATATCCAGTCCTCGCCTGATTACTACGAATCAACAGGCAGCAGTCATAGAGTCTGGTGAGGAGATACCCTATCAGGAG
GCAACATCAAGTGGAGCGACTGCTGTTGCATTTAAAAAAGCGGTATTGAGTTTGAAAGTTACCCCACAAATTACGCCTGA
TGGTAAAATTTTGATGGATTTACAGATAAATCAGGATACTCCTTCCCCACAAACATTCCTTGGTGTTCCAGCAATAATAA
CAAAGGAGATTCAGACGAATGTTCTGGTTAATAATGGACAAACCATAGTACTTGGGGGTATTTATAAGCAAGATAAGAAC
AAGGTAATCAATAGAATTCCTTTCTTTGGTGAGTTGCCTGTTGTCGGTATTTTATTTAGTAATAGACAAATCACATTGAA
AAATGAGGAATTACTGATTTTTATTACTCCAAGAATAATAACGAATGCCTTATCGATTACAACGATAGAGGGACGTGAAA
AGGATGTTTATAAATAA

Domains

Predicted by InterproScan.

• AMIN domain (AMIN) (39-113)

• Bacterial type II/III secretion system short domain (Secretin_N) (370-433)

• Bacterial type II and III secretion system protein (Secretin) (525-680)

3D structure

• AlphaFold DB: Q5ZX02

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilQ	Legionella pneumophila strain ERS1305867	99.57	100	0.996
pilQ	Vibrio cholerae O1 biovar El Tor strain E7946	42.778	94.571	0.405
pilQ	Vibrio cholerae strain A1552	42.778	94.571	0.405
pilQ	Vibrio campbellii strain DS40M4	41.894	94.655	0.397
pilQ	Pseudomonas aeruginosa PAK	37.73	100	0.381
comQ	Acinetobacter baylyi ADP1	38.428	98.424	0.378
pilQ	Acinetobacter baumannii D1279779	35.095	100	0.371
pilQ	Acinetobacter baumannii strain A118	35.095	100	0.371

References

• [1]: Sebastian A G Braus et al. (2022) The molecular basis of FimT-mediated DNA uptake during bacterial natural transformation. Nature Communications 13(1):1065. [PMID: 35246533]