Detailed information NTG0001257

Experimentally validated

Overview

• Name: pilQ
• Type: Machinery gene
• Locus tag: GCO85_RS04855
• Genome accession: NZ_CP048618
• Coordinates: 1056829..1058925 (+)
• Length: 698 a.a.
• NCBI ID: WP_163621000.1
• Uniprot ID: -
• Organism: Legionella pneumophila strain ERS1305867
• Function: assembly of type IV pilus DNA binding and uptake

Function

Mutation of this gene caused a decrease in transformation.

Genomic Context

Location: 1051829..1063925

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GCO85_RS04830 (GCO85_04840)	ravI	1052591..1053598 (-)	1008	WP_011946020.1	Dot/Icm T4SS effector RavI	-
GCO85_RS04835 (GCO85_04845)	pilM	1054015..1055079 (+)	1065	WP_011215094.1	type IV pilus assembly protein PilM	Machinery gene
GCO85_RS04840 (GCO85_04850)	pilN	1055083..1055631 (+)	549	WP_011213354.1	PilN domain-containing protein	Machinery gene
GCO85_RS04845 (GCO85_04855)	pilO	1055645..1056244 (+)	600	WP_011213355.1	type 4a pilus biogenesis protein PilO	Machinery gene
GCO85_RS04850 (GCO85_04860)	pilP	1056241..1056825 (+)	585	WP_011213356.1	pilus assembly protein PilP	Machinery gene
GCO85_RS04855 (GCO85_04865)	pilQ	1056829..1058925 (+)	2097	WP_163621000.1	type IV pilus secretin PilQ	Machinery gene
GCO85_RS04860 (GCO85_04870)	aroK	1059378..1059899 (+)	522	WP_027219629.1	shikimate kinase AroK	-
GCO85_RS04865 (GCO85_04875)	aroB	1059886..1060995 (+)	1110	WP_013101269.1	3-dehydroquinate synthase	-
GCO85_RS04870 (GCO85_04880)	-	1060992..1062443 (+)	1452	WP_027219627.1	SPOR domain-containing protein	-
GCO85_RS04875 (GCO85_04885)	-	1062552..1062983 (+)	432	WP_010946670.1	universal stress protein	-

Sequence

Protein

• Length: 698 a.a.
• Molecular weight: 77483.79 Da
• Isoelectric Point: 9.5345

>NTDB_id=1257 GCO85_RS04855 WP_163621000.1 1056829..1058925(+) (pilQ) [Legionella pneumophila strain ERS1305867]
MRKIIAILILISASLSLAYSKDNSLISVKVLPLPENRVRIDFQFSKPLKQLPASFITQKPPRLVLDFISTDMQIPSDQKT
KTIQLGSLHSYNIVAVGDRVRAILELDRSVSYSGSTAGSVYSLVLNGKSNDLFENSKEVFITNQVVNAKHEIKRIDFRGI
EKQGGRVLIDVSDTGIPIDVTQVGKEIVVNFLNTKIPLNLMKRYDVSDFHSPVQIITMQQEGKKVRMTILSKGDYGHFVY
QVNKQFMVDVFPLTAEEIRQAKLKKQVFTGKRISLNFQNISIRAVLQLLADFTGINMVVSDKVQGDITLRLNDIPWDQAL
DIILTTQGLDKRRKGNVMLIDTKASMDKMEEEQLKSQQTIEKLEPIRSDLIQINYAKAADIAVLIKDKQNSLLSDRGKIS
VDARTNTIWIQDTGTKIEEVRELIKQLDIPVKQVLIEARIVEVTKDFSQDIGIRWGVSRPTHLSGTLEGANELARGTAPA
NVVPLERRLNLDLAAVPLTGATPASVGIALAKLNDNILLDLELSALESEGRAELISSPRLITTNQQAAVIESGEEIPYQE
ATSSGATAVAFKKAVLSLKVTPQITPDGKILMDLQINQDTPSPQTFLGVPAIITKEIQTNVLVNNGQTIVLGGIYKQDKN
KVINRIPFFGELPVVGILFSNRQITLKNEELLIFITPRIITNALSITTIEGREKDVYK

Nucleotide

• Length: 2097 bp

>NTDB_id=1257 GCO85_RS04855 WP_163621000.1 1056829..1058925(+) (pilQ) [Legionella pneumophila strain ERS1305867]
GTGAGAAAAATTATCGCGATTTTGATATTAATTAGTGCCAGTTTGAGCCTGGCATATTCTAAAGATAATTCGCTCATTTC
AGTAAAAGTATTGCCATTGCCCGAAAACAGAGTACGTATTGATTTTCAATTTTCCAAGCCCTTAAAGCAGTTGCCCGCAA
GCTTTATTACTCAAAAGCCGCCTCGTTTGGTGTTAGATTTTATCAGCACTGATATGCAAATTCCCTCGGATCAGAAAACT
AAAACTATTCAATTAGGCTCATTACACAGTTATAACATTGTTGCTGTTGGTGATAGAGTAAGAGCCATATTGGAACTGGA
TCGTTCAGTGTCCTATTCTGGCAGTACGGCTGGTTCTGTTTATAGCCTTGTCCTGAATGGTAAAAGCAATGATTTATTTG
AAAATAGCAAGGAGGTATTCATAACGAACCAGGTTGTGAACGCCAAGCATGAAATTAAAAGAATTGATTTTCGTGGTATA
GAAAAGCAGGGAGGTAGAGTTTTAATTGATGTTTCTGATACGGGTATTCCAATTGATGTAACTCAAGTTGGGAAAGAAAT
AGTCGTTAACTTCCTGAATACCAAGATCCCTTTGAATTTAATGAAACGGTATGATGTTTCTGATTTTCACAGCCCTGTGC
AGATTATTACGATGCAACAGGAAGGCAAAAAGGTTCGCATGACCATCCTCAGCAAAGGGGATTATGGACATTTTGTTTAT
CAGGTCAATAAACAATTTATGGTCGATGTCTTTCCTTTAACTGCGGAAGAAATTCGTCAGGCGAAATTGAAGAAACAGGT
ATTTACCGGTAAACGGATTTCTTTGAATTTCCAGAATATTAGTATTCGGGCTGTGCTTCAACTGCTGGCAGATTTTACCG
GAATCAATATGGTTGTCAGCGACAAAGTGCAAGGAGATATTACGCTTAGATTAAATGATATCCCCTGGGATCAGGCTCTT
GATATCATTTTAACCACACAAGGGCTAGATAAGCGCAGAAAGGGCAACGTCATGTTGATAGATACCAAAGCCTCCATGGA
TAAAATGGAGGAAGAACAACTCAAAAGTCAACAGACAATTGAAAAACTTGAGCCTATCCGATCTGATTTAATACAGATTA
ATTATGCAAAGGCAGCAGATATTGCTGTTTTGATCAAGGATAAGCAAAACTCCCTATTATCAGACAGAGGAAAAATAAGT
GTTGATGCCAGAACGAATACAATCTGGATTCAGGATACTGGCACCAAAATTGAGGAAGTAAGGGAACTGATAAAGCAATT
GGATATCCCTGTTAAGCAAGTGCTTATTGAGGCAAGAATTGTTGAAGTAACTAAAGATTTTAGTCAAGATATTGGGATAC
GCTGGGGGGTATCAAGACCAACTCACTTAAGCGGTACATTGGAAGGTGCTAACGAGTTAGCGAGAGGAACAGCACCAGCT
AATGTTGTCCCCTTGGAAAGACGATTAAATCTTGATTTGGCAGCTGTTCCTTTAACTGGAGCTACACCGGCCTCGGTAGG
TATTGCTCTAGCCAAGCTTAATGATAACATTCTGCTTGATCTGGAATTGTCTGCTTTGGAAAGTGAAGGGCGTGCTGAAT
TAATATCCAGTCCCCGCCTGATTACTACGAATCAACAGGCAGCAGTCATCGAGTCTGGTGAGGAGATTCCCTATCAGGAG
GCAACATCGAGTGGAGCGACGGCTGTTGCATTTAAAAAAGCAGTGTTGAGTTTGAAAGTTACCCCGCAAATTACGCCTGA
CGGTAAAATTTTGATGGATTTACAGATAAATCAGGATACTCCTTCTCCACAAACATTCCTTGGTGTTCCAGCAATAATAA
CAAAGGAGATTCAGACGAATGTTCTGGTTAATAATGGACAAACCATAGTACTTGGGGGTATTTATAAGCAAGATAAGAAC
AAGGTAATCAATAGAATTCCTTTCTTTGGTGAGTTGCCTGTTGTCGGTATTTTATTTAGTAATAGACAAATCACATTGAA
AAATGAAGAATTACTTATTTTTATTACACCAAGAATAATAACGAATGCCCTATCGATAACAACGATAGAAGGACGTGAAA
AAGATGTTTATAAATAA

Domains

Predicted by InterproScan.

• Bacterial type II and III secretion system protein (Secretin) (525-680)

• Bacterial type II/III secretion system short domain (Secretin_N) (370-433)

• AMIN domain (AMIN) (39-113)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilQ	Legionella pneumophila strain Lp02	99.57	100	0.996
pilQ	Vibrio cholerae strain A1552	42.778	94.571	0.405
pilQ	Vibrio cholerae O1 biovar El Tor strain E7946	42.778	94.571	0.405
pilQ	Vibrio campbellii strain DS40M4	41.894	94.655	0.397
comQ	Acinetobacter baylyi ADP1	38.719	98.424	0.381
pilQ	Pseudomonas aeruginosa PAK	37.73	100	0.381

References

• [1]: Léo Hardy et al. (2021) Transposon Insertion Sequencing in a Clinical Isolate of Legionella pneumophila Identifies Essential Genes and Determinants of Natural Transformation. Journal of Bacteriology 203(3):e00548-20. [PMID: 33168636]