Detailed information    

experimental Experimentally validated

Overview

Name   pilM   Type   Machinery gene
Locus tag   GCO85_RS04835 Genome accession   NZ_CP048618
Coordinates   1054015..1055079 (+) Length   354 a.a.
NCBI ID   WP_011215094.1    Uniprot ID   Q5WXY1
Organism   Legionella pneumophila strain ERS1305867     
Function   assembly of type IV pilus   
DNA binding and uptake

Function

Mutation of this gene caused a decrease in transformation.


Genomic Context

Location: 1049015..1060079
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GCO85_RS04825 (GCO85_04835) - 1050055..1052439 (-) 2385 WP_027220940.1 penicillin-binding protein 1A -
  GCO85_RS04830 (GCO85_04840) ravI 1052591..1053598 (-) 1008 WP_011946020.1 Dot/Icm T4SS effector RavI -
  GCO85_RS04835 (GCO85_04845) pilM 1054015..1055079 (+) 1065 WP_011215094.1 type IV pilus assembly protein PilM Machinery gene
  GCO85_RS04840 (GCO85_04850) pilN 1055083..1055631 (+) 549 WP_011213354.1 PilN domain-containing protein Machinery gene
  GCO85_RS04845 (GCO85_04855) pilO 1055645..1056244 (+) 600 WP_011213355.1 type 4a pilus biogenesis protein PilO Machinery gene
  GCO85_RS04850 (GCO85_04860) pilP 1056241..1056825 (+) 585 WP_011213356.1 pilus assembly protein PilP Machinery gene
  GCO85_RS04855 (GCO85_04865) pilQ 1056829..1058925 (+) 2097 WP_163621000.1 type IV pilus secretin PilQ Machinery gene
  GCO85_RS04860 (GCO85_04870) aroK 1059378..1059899 (+) 522 WP_027219629.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38760.78 Da        Isoelectric Point: 5.0367

>NTDB_id=1253 GCO85_RS04835 WP_011215094.1 1054015..1055079(+) (pilM) [Legionella pneumophila strain ERS1305867]
MLKLLKPKHRSILGIDISSSAVKILEISGSGEDICVEGYGREEIPASALEGNMIKDVDVVANCIKKLVERLKFTSKNVAL
AVPDSAVISKVVQINEGLTDLEMEELVVIEADKYIPYPIDEINLDFEVLGHSAKSSSLLDVLIVASRAENVNTRVEAAHR
AGLEPKVVDVESYAVERSVQQIAQELPASGQDKIVAIVDIGANYTHLFVLHGMRLIFSREEKFGGMQLVEAIAEHYHMNL
EQAIALKNQGKLPEDYESAVLEPFKDMILLQIKRTLQFFYSTSQHGFVDHILLAGGMARQPGLAALIQEQLGVTTTVANP
LSHMSFGKKVNLDVINSDAPTLMVACGLALRHVE

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=1253 GCO85_RS04835 WP_011215094.1 1054015..1055079(+) (pilM) [Legionella pneumophila strain ERS1305867]
GTGCTCAAATTGCTTAAACCTAAGCATCGTTCAATTCTTGGCATTGACATCAGTTCTTCCGCAGTGAAAATTCTGGAAAT
ATCAGGTTCCGGAGAGGACATTTGTGTTGAAGGATATGGTCGTGAAGAAATACCTGCCTCTGCCCTAGAAGGAAATATGA
TCAAGGATGTCGATGTAGTAGCGAATTGCATCAAAAAATTGGTAGAAAGATTGAAGTTTACATCTAAAAATGTAGCATTG
GCTGTACCTGATTCGGCTGTTATCAGTAAAGTAGTGCAAATTAATGAAGGGTTAACTGACCTTGAAATGGAAGAGCTTGT
CGTTATTGAGGCGGATAAGTACATTCCTTACCCAATAGATGAAATAAACCTGGATTTTGAAGTGTTAGGCCATTCAGCCA
AGAGCTCATCACTGCTTGATGTTTTAATCGTCGCATCAAGGGCAGAAAACGTTAATACCCGTGTTGAAGCAGCCCATAGA
GCAGGGCTGGAACCCAAAGTTGTTGACGTTGAATCTTATGCAGTAGAGCGTTCAGTACAACAAATTGCGCAAGAATTACC
AGCAAGTGGGCAAGACAAAATTGTTGCAATAGTAGATATTGGAGCCAACTATACTCACTTATTTGTTTTGCATGGTATGC
GACTAATCTTTTCAAGAGAAGAAAAATTTGGAGGTATGCAACTTGTTGAAGCTATTGCAGAGCACTATCATATGAATTTG
GAGCAGGCTATTGCCCTAAAAAATCAGGGTAAACTACCGGAGGACTACGAATCTGCTGTATTAGAACCTTTTAAGGACAT
GATTCTTTTGCAGATCAAACGTACTTTGCAGTTTTTTTATTCAACCAGTCAACATGGATTTGTAGACCATATTTTATTGG
CGGGAGGAATGGCAAGACAACCAGGATTGGCAGCGCTTATTCAAGAACAGTTAGGAGTGACTACTACAGTGGCAAATCCA
CTATCCCATATGTCTTTTGGTAAAAAAGTGAATTTGGATGTGATTAACAGTGATGCACCTACACTGATGGTGGCTTGTGG
GTTGGCTTTGAGGCATGTCGAGTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-352)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q5WXY1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comM Acinetobacter nosocomialis M2

42.373

100

0.426
  pilM Acinetobacter baumannii D1279779

42.09

100

0.423
  comM Acinetobacter baylyi ADP1

40.678

100

0.408

Multiple sequence alignment    



References

[1] Léo Hardy et al. (2021) Transposon Insertion Sequencing in a Clinical Isolate of Legionella pneumophila Identifies Essential Genes and Determinants of Natural Transformation. Journal of Bacteriology 203(3):e00548-20. [PMID: 33168636]