Detailed information NTG0001084

Experimentally validated

Overview

• Name: comM
• Type: Machinery gene
• Locus tag: FDQ49_RS11070
• Genome accession: NZ_CP040105
• Coordinates: 2349600..2350658 (+)
• Length: 352 a.a.
• NCBI ID: WP_004711654.1
• Uniprot ID: A0AAV3IQ41
• Organism: Acinetobacter nosocomialis M2
• Function: assembly of type IV pilus DNA binding and uptake

Function

Require for natural competence. Nearly all AbaRs specifically disrupt comM.

Genomic Context

Location: 2344600..2355658

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FDQ49_RS11055 (FDQ49_11055)	-	2345703..2346035 (+)	333	WP_022576003.1	hypothetical protein	-
FDQ49_RS11060 (FDQ49_11060)	-	2346038..2346862 (+)	825	WP_086373946.1	putative RNA methyltransferase	-
FDQ49_RS11065 (FDQ49_11065)	ponA	2346880..2349438 (-)	2559	WP_022576001.1	penicillin-binding protein PBP1a	-
FDQ49_RS11070 (FDQ49_11070)	comM	2349600..2350658 (+)	1059	WP_004711654.1	pilus assembly protein PilM	Machinery gene
FDQ49_RS11075 (FDQ49_11075)	pilN	2350658..2351302 (+)	645	WP_004711656.1	PilN domain-containing protein	Machinery gene
FDQ49_RS11080 (FDQ49_11080)	pilO	2351299..2352039 (+)	741	WP_006581061.1	type 4a pilus biogenesis protein PilO	Machinery gene
FDQ49_RS11085 (FDQ49_11085)	pilP	2352050..2352577 (+)	528	WP_004711660.1	pilus assembly protein PilP	Machinery gene
FDQ49_RS11090 (FDQ49_11090)	pilQ	2352640..2354805 (+)	2166	WP_006581062.1	type IV pilus secretin PilQ	Machinery gene
FDQ49_RS11095 (FDQ49_11095)	aroK	2354817..2355386 (+)	570	WP_002051689.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 39065.66 Da
• Isoelectric Point: 4.4177

>NTDB_id=1084 FDQ49_RS11070 WP_004711654.1 2349600..2350658(+) (comM) [Acinetobacter nosocomialis M2]
MLRLYRKPNKGLMGVDISSTSVKLLELSVKNGKYWVESYALMPLPENSVVEKNILNPEAVAEALERAMNLANPQTTNAAI
AVPTSTVIHKTIEMDADMSDDEREVQIRVDAEQYIPFPLDEVSLDFEVLPDRLLNPNRVNVLLVATRTENVETRVEVLEL
ADLNPKLADVESYAVERAFSVFADSLPMGANTIGILDIGHTMTTLSVMQNGKIIYTREQVFGGKQLTLEIQSRYGLSLEE
ASRAKKDRTLPDDYEIEVLDPFLDAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQVDVQKIENDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=1084 FDQ49_RS11070 WP_004711654.1 2349600..2350658(+) (comM) [Acinetobacter nosocomialis M2]
GTGCTCAGGTTATATCGTAAACCTAATAAGGGGTTAATGGGCGTCGATATTAGTTCGACTTCTGTTAAGTTGTTAGAGCT
CTCTGTCAAGAACGGTAAATATTGGGTAGAAAGCTATGCTTTGATGCCTTTACCTGAAAACAGTGTAGTTGAAAAAAATA
TCTTAAATCCAGAAGCAGTAGCAGAAGCTTTGGAACGGGCGATGAATTTAGCAAATCCCCAAACCACTAATGCTGCAATT
GCTGTTCCAACATCGACGGTTATTCATAAAACTATCGAAATGGATGCAGATATGAGCGATGACGAACGTGAAGTTCAGAT
TCGTGTAGATGCGGAGCAGTACATACCGTTCCCGCTGGATGAGGTGAGCCTTGATTTTGAGGTTTTGCCTGATCGCTTGC
TGAATCCAAATCGAGTAAATGTTCTTTTGGTCGCAACAAGAACGGAAAACGTTGAAACACGTGTTGAAGTTCTTGAATTG
GCAGATTTGAATCCTAAACTGGCAGATGTCGAGAGTTATGCAGTCGAACGTGCTTTTAGTGTGTTTGCTGATAGCTTGCC
GATGGGCGCAAATACGATAGGAATTTTAGATATCGGCCATACCATGACAACACTATCGGTCATGCAAAATGGCAAGATTA
TTTATACTCGTGAGCAGGTTTTTGGTGGTAAGCAACTTACGCTTGAAATTCAAAGTCGTTATGGCCTATCTCTAGAAGAA
GCAAGCCGAGCTAAAAAAGACCGTACTTTGCCAGATGATTATGAAATTGAGGTACTAGATCCATTTCTAGATGCTGTGGT
TCAGCAGGCGGCCCGCTCATTACAATTTTTCTTTTCTTCATCTCAGTTTAACGAAATAGACCATATTTTGCTTGCAGGTG
GCAATGCGAATATTCCAGGTCTTGCCAAGCTTTTGCAGCAAAAATTAGGCTACCGTGTCACTATTGCCAACCCATTTTTA
CAAATGGGTTTTTCTCCTCAAGTCGACGTTCAAAAAATTGAAAATGATGCTTCATCTTTAATGGTGGCATGCGGCTTGGC
TTTAAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	99.148	100	0.991
comM	Acinetobacter baylyi ADP1	81.25	100	0.813
pilM	Legionella pneumophila strain ERS1305867	42.373	100	0.426

References

• [1]: Rémi Tuffet et al. (2024) Manipulation of natural transformation by AbaR-type islands promotes fixation of antibiotic resistance in Acinetobacter baumannii. Proceedings of The National Academy of Sciences of The United States of America 121(39):e2409843121. [PMID: 39288183]