Detailed information    

experimental Experimentally validated

Overview

Name   pilM   Type   Machinery gene
Locus tag   ABD1_RS15870 Genome accession   NC_020547
Coordinates   3353171..3354229 (-) Length   352 a.a.
NCBI ID   WP_002014066.1    Uniprot ID   N9LI98
Organism   Acinetobacter baumannii D1279779     
Function   assembly of type IV pilus   
DNA binding and uptake

Function

We examined the 33 knockout mutants by comparing their transformation efficiency with wild-type W068. No knockout strains were affected in growth, but 28 of these mutants were severely or completely impaired in natural transformability. These mutations were in 18 genes related to T4P (pilF, pilQ, tsaP, pilM, pilN, pilO, pilP, pilB, pilC, pilT, pilD, pilE, pilY2, pilY1, pilX, pilW, pilV, and fimU), six genes related to DNA uptake and processing (comEA, comA, comF, priA, dprA, and recA), two genes related to T2SS (xcpV and xcpW), and the crp and tonB2 genes.


Genomic Context

Location: 3348171..3359229
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABD1_RS15845 (ABD1_30750) aroK 3348446..3349015 (-) 570 WP_000643712.1 shikimate kinase AroK -
  ABD1_RS15850 (ABD1_30760) pilQ 3349027..3351192 (-) 2166 WP_001017033.1 type IV pilus secretin PilQ family protein Machinery gene
  ABD1_RS15855 (ABD1_30770) pilP 3351255..3351782 (-) 528 WP_000695065.1 pilus assembly protein PilP Machinery gene
  ABD1_RS15860 (ABD1_30780) pilO 3351793..3352533 (-) 741 WP_000076101.1 type 4a pilus biogenesis protein PilO Machinery gene
  ABD1_RS15865 (ABD1_30790) pilN 3352530..3353171 (-) 642 WP_000201227.1 PilN domain-containing protein Machinery gene
  ABD1_RS15870 (ABD1_30800) pilM 3353171..3354229 (-) 1059 WP_002014066.1 pilus assembly protein PilM Machinery gene
  ABD1_RS15875 (ABD1_30810) ponA 3354391..3356946 (+) 2556 WP_000736674.1 penicillin-binding protein PBP1a -
  ABD1_RS15880 (ABD1_30820) - 3356964..3357788 (-) 825 WP_001990541.1 putative RNA methyltransferase -
  ABD1_RS15885 (ABD1_30830) - 3357791..3358123 (-) 333 WP_000169598.1 DUF7709 family protein -

Sequence

Protein


Download         Length: 352 a.a.        Molecular weight: 39032.59 Da        Isoelectric Point: 4.4618

>NTDB_id=1054 ABD1_RS15870 WP_002014066.1 3353171..3354229(-) (pilM) [Acinetobacter baumannii D1279779]
MLRLYRKPNKGLMGVDISSTSVKLLELSVKNGKYWVESYALMPLPENSVVEKNILNPEAVAEALERAMNLANPQTTHAAI
AVPTSTVIHKTIEMDADMSDDEREVQIRVDAEQYIPFPLDEVSLDFEVLPDRLANPNRVNVLLVATRTENVETRVEVLEL
ADLNPKLADVESYAVERAFSVFADSLPMGANTIGILDIGHTMTTLSVMQNGKIIYTREQVFGGKQLTLEIQSRYGLSLEE
ASRAKKDRSLPDDYEIEVLDPFLDAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQVDVQKIENDASSLMVACGLALRSFD

Nucleotide


Download         Length: 1059 bp        

>NTDB_id=1054 ABD1_RS15870 WP_002014066.1 3353171..3354229(-) (pilM) [Acinetobacter baumannii D1279779]
GTGCTCAGGTTATATCGTAAACCTAATAAGGGGTTAATGGGTGTCGATATTAGTTCGACTTCTGTTAAGTTGTTAGAGCT
CTCTGTCAAGAACGGTAAATATTGGGTAGAAAGCTATGCTTTGATGCCTTTACCCGAAAACAGTGTAGTTGAAAAAAATA
TCTTAAATCCAGAAGCAGTTGCAGAGGCTTTGGAACGGGCGATGAATTTAGCAAATCCCCAAACCACTCATGCTGCAATT
GCTGTTCCGACATCGACGGTTATTCATAAAACTATCGAAATGGATGCAGATATGAGCGATGACGAACGCGAAGTTCAGAT
TCGTGTAGATGCGGAGCAGTATATACCGTTCCCTTTAGATGAGGTGAGCCTTGATTTTGAGGTTTTGCCGGATCGTCTTG
CGAATCCAAATCGTGTAAATGTGCTCTTGGTCGCCACAAGAACAGAAAACGTTGAAACACGCGTTGAAGTGCTTGAATTG
GCAGATTTAAATCCTAAATTGGCTGATGTCGAAAGTTACGCGGTTGAGCGCGCTTTTAGCGTGTTTGCTGATAGCTTACC
GATGGGTGCAAATACCATAGGGATTTTAGATATCGGTCATACCATGACGACATTATCTGTCATGCAAAATGGCAAGATTA
TTTATACACGAGAGCAGGTTTTCGGCGGAAAACAACTTACGCTTGAAATTCAAAGTCGTTATGGTTTGTCTTTAGAAGAA
GCAAGTCGAGCAAAAAAAGATCGCTCTTTACCAGATGATTATGAAATTGAAGTGCTAGACCCATTTCTAGATGCGGTAGT
TCAGCAGGCGGCCCGTTCACTACAATTTTTCTTTTCTTCATCCCAATTTAACGAAATAGACCATATTTTGCTCGCTGGTG
GAAATGCGAATATTCCAGGCCTTGCTAAGCTTTTGCAGCAAAAATTGGGCTACCGTGTCACGATTGCCAACCCGTTTTTA
CAAATGGGCTTTTCTCCTCAAGTCGATGTTCAAAAAATTGAAAATGATGCTTCATCTTTAATGGTGGCATGTGGCTTGGC
TTTAAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-351)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB N9LI98

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comM Acinetobacter nosocomialis M2

99.148

100

0.991
  comM Acinetobacter baylyi ADP1

81.534

100

0.815
  pilM Legionella pneumophila strain ERS1305867

42.09

100

0.423

Multiple sequence alignment    



References

[1] Yuan Hu et al. (2021) Natural Transformation in Acinetobacter baumannii W068: A Genetic Analysis Reveals the Involvements of the CRP, XcpV, XcpW, TsaP, and TonB2. Frontiers in Microbiology 12:738034. [PMID: 35126321]