Detailed information    

experimental Experimentally validated

Overview

Name   pilP   Type   Machinery gene
Locus tag   ABD1_RS15855 Genome accession   NC_020547
Coordinates   3351255..3351782 (-) Length   175 a.a.
NCBI ID   WP_000695065.1    Uniprot ID   N9K1N5
Organism   Acinetobacter baumannii D1279779     
Function   assembly of type IV pilus   
DNA binding and uptake

Function

We examined the 33 knockout mutants by comparing their transformation efficiency with wild-type W068. No knockout strains were affected in growth, but 28 of these mutants were severely or completely impaired in natural transformability. These mutations were in 18 genes related to T4P (pilF, pilQ, tsaP, pilM, pilN, pilO, pilP, pilB, pilC, pilT, pilD, pilE, pilY2, pilY1, pilX, pilW, pilV, and fimU), six genes related to DNA uptake and processing (comEA, comA, comF, priA, dprA, and recA), two genes related to T2SS (xcpV and xcpW), and the crp and tonB2 genes.


Genomic Context

Location: 3346255..3356782
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABD1_RS15835 (ABD1_30730) - 3346444..3347319 (-) 876 WP_000218807.1 hypothetical protein -
  ABD1_RS15840 (ABD1_30740) aroB 3347335..3348417 (-) 1083 WP_001193999.1 3-dehydroquinate synthase -
  ABD1_RS15845 (ABD1_30750) aroK 3348446..3349015 (-) 570 WP_000643712.1 shikimate kinase AroK -
  ABD1_RS15850 (ABD1_30760) pilQ 3349027..3351192 (-) 2166 WP_001017033.1 type IV pilus secretin PilQ family protein Machinery gene
  ABD1_RS15855 (ABD1_30770) pilP 3351255..3351782 (-) 528 WP_000695065.1 pilus assembly protein PilP Machinery gene
  ABD1_RS15860 (ABD1_30780) pilO 3351793..3352533 (-) 741 WP_000076101.1 type 4a pilus biogenesis protein PilO Machinery gene
  ABD1_RS15865 (ABD1_30790) pilN 3352530..3353171 (-) 642 WP_000201227.1 PilN domain-containing protein Machinery gene
  ABD1_RS15870 (ABD1_30800) pilM 3353171..3354229 (-) 1059 WP_002014066.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 175 a.a.        Molecular weight: 19419.64 Da        Isoelectric Point: 8.9898

>NTDB_id=1057 ABD1_RS15855 WP_000695065.1 3351255..3351782(-) (pilP) [Acinetobacter baumannii D1279779]
MKIKQITLYVLACAVLVGCDSRIDAVNQEMANIRNQPPLPIEPAPIFTPVPQFNYAAHQLKSPFMPSSLAAELKIMAGKR
VYPNFSRAPQPLESYALEALNMKGSMRNNRGQILALIQTPDQQIERVQVGNYMGMNQGRITHISPTQIDLVEIVPDGREG
YVERPRTLVLIGPAP

Nucleotide


Download         Length: 528 bp        

>NTDB_id=1057 ABD1_RS15855 WP_000695065.1 3351255..3351782(-) (pilP) [Acinetobacter baumannii D1279779]
ATGAAAATTAAGCAAATTACTCTTTATGTTTTGGCGTGTGCTGTGCTTGTCGGTTGTGATTCAAGAATTGATGCAGTAAA
TCAGGAAATGGCCAATATTCGTAATCAGCCGCCACTCCCTATTGAACCAGCACCGATTTTTACTCCTGTACCACAATTTA
ATTACGCTGCTCATCAATTAAAAAGCCCATTTATGCCAAGTTCTCTAGCTGCCGAGCTCAAGATTATGGCGGGCAAACGG
GTTTATCCGAATTTCTCACGTGCGCCGCAACCACTCGAAAGTTATGCACTTGAAGCTTTAAATATGAAAGGGAGCATGCG
TAATAATCGAGGGCAGATATTGGCCCTGATCCAGACACCGGATCAGCAGATTGAACGGGTACAAGTGGGTAACTATATGG
GCATGAATCAGGGCCGTATTACCCATATTAGTCCAACCCAGATTGATCTGGTTGAAATCGTACCTGATGGTCGAGAAGGC
TATGTCGAAAGACCAAGAACCTTGGTTTTGATTGGGCCTGCACCCTAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilP (PilP)

(27-170)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB N9K1N5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comL Acinetobacter baylyi ADP1

71.839

100

0.718

Multiple sequence alignment    



References

[1] Yuan Hu et al. (2021) Natural Transformation in Acinetobacter baumannii W068: A Genetic Analysis Reveals the Involvements of the CRP, XcpV, XcpW, TsaP, and TonB2. Frontiers in Microbiology 12:738034. [PMID: 35126321]