Detailed information NTG0001056

Experimentally validated

Overview

• Name: pilO
• Type: Machinery gene
• Locus tag: ABD1_RS15860
• Genome accession: NC_020547
• Coordinates: 3351793..3352533 (-)
• Length: 246 a.a.
• NCBI ID: WP_000076101.1
• Uniprot ID: N9LH62
• Organism: Acinetobacter baumannii D1279779
• Function: assembly of type IV pilus DNA binding and uptake

Function

We examined the 33 knockout mutants by comparing their transformation efficiency with wild-type W068. No knockout strains were affected in growth, but 28 of these mutants were severely or completely impaired in natural transformability. These mutations were in 18 genes related to T4P (pilF, pilQ, tsaP, pilM, pilN, pilO, pilP, pilB, pilC, pilT, pilD, pilE, pilY2, pilY1, pilX, pilW, pilV, and fimU), six genes related to DNA uptake and processing (comEA, comA, comF, priA, dprA, and recA), two genes related to T2SS (xcpV and xcpW), and the crp and tonB2 genes.

Genomic Context

Location: 3346793..3357533

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ABD1_RS15840 (ABD1_30740)	aroB	3347335..3348417 (-)	1083	WP_001193999.1	3-dehydroquinate synthase	-
ABD1_RS15845 (ABD1_30750)	aroK	3348446..3349015 (-)	570	WP_000643712.1	shikimate kinase AroK	-
ABD1_RS15850 (ABD1_30760)	pilQ	3349027..3351192 (-)	2166	WP_001017033.1	type IV pilus secretin PilQ family protein	Machinery gene
ABD1_RS15855 (ABD1_30770)	pilP	3351255..3351782 (-)	528	WP_000695065.1	pilus assembly protein PilP	Machinery gene
ABD1_RS15860 (ABD1_30780)	pilO	3351793..3352533 (-)	741	WP_000076101.1	type 4a pilus biogenesis protein PilO	Machinery gene
ABD1_RS15865 (ABD1_30790)	pilN	3352530..3353171 (-)	642	WP_000201227.1	PilN domain-containing protein	Machinery gene
ABD1_RS15870 (ABD1_30800)	pilM	3353171..3354229 (-)	1059	WP_002014066.1	pilus assembly protein PilM	Machinery gene
ABD1_RS15875 (ABD1_30810)	ponA	3354391..3356946 (+)	2556	WP_000736674.1	penicillin-binding protein PBP1a	-

Sequence

Protein

• Length: 246 a.a.
• Molecular weight: 27819.59 Da
• Isoelectric Point: 4.8357

>NTDB_id=1056 ABD1_RS15860 WP_000076101.1 3351793..3352533(-) (pilO) [Acinetobacter baumannii D1279779]
MSQDELQELSLEQLSAKKKKFNLDKFLQQFNTLDMNNYGSWPISVKITCWIFIFFAVLALGYFIVIQPKLQAIDNAQAQE
SNLLNEFREKDSKLRNLQQYQVQLQEMQANFNQQLEQLPKETEIPSLVEDINLTGVNSGLKFKNIRLEDEVKQEIFIEQP
IAIEATGDYHAFGAFVSSIAALPRIVTMHDFTVDVSPAKDNKSDIPVLNYSIKAKTYRYVGATDTSEQTGKAPAASASTN
TTVQPK

Nucleotide

• Length: 741 bp

>NTDB_id=1056 ABD1_RS15860 WP_000076101.1 3351793..3352533(-) (pilO) [Acinetobacter baumannii D1279779]
ATGAGTCAAGACGAACTTCAAGAATTGTCATTAGAACAATTATCTGCAAAGAAAAAGAAATTTAATCTCGATAAATTCCT
TCAGCAATTTAATACGCTGGATATGAACAACTACGGTAGTTGGCCAATATCTGTAAAAATTACGTGTTGGATTTTTATTT
TCTTTGCTGTGTTAGCTTTGGGGTATTTCATTGTTATTCAACCAAAATTACAAGCTATTGATAATGCTCAAGCTCAGGAA
AGCAATTTATTAAACGAATTTCGTGAAAAAGACTCGAAATTACGTAACCTGCAACAGTATCAGGTACAGCTTCAAGAAAT
GCAGGCAAATTTCAACCAGCAACTAGAGCAGTTACCAAAAGAAACTGAGATTCCGAGTTTAGTTGAAGATATCAACTTAA
CAGGAGTGAACTCTGGATTGAAATTTAAAAATATTCGCTTAGAAGATGAAGTAAAGCAGGAAATTTTTATTGAGCAACCA
ATTGCCATAGAAGCGACCGGGGACTATCACGCATTTGGAGCTTTTGTAAGTAGTATCGCTGCTTTGCCTCGTATTGTAAC
CATGCACGATTTTACTGTTGATGTATCGCCAGCTAAAGATAACAAGTCTGATATTCCTGTGCTGAACTATTCCATTAAAG
CAAAAACATACCGATACGTAGGGGCAACAGATACATCGGAGCAAACAGGCAAAGCTCCAGCAGCATCTGCTTCAACGAAC
ACTACTGTTCAGCCGAAATAG

Domains

Predicted by InterproScan.

• Pilus assembly protein, PilO (PilO) (73-220)

3D structure

• AlphaFold DB: N9LH62

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comO	Acinetobacter baylyi ADP1	69.421	98.776	0.686
pilO	Legionella pneumophila strain ERS1305867	38.22	95.98	0.367

References

• [1]: Yuan Hu et al. (2021) Natural Transformation in Acinetobacter baumannii W068: A Genetic Analysis Reveals the Involvements of the CRP, XcpV, XcpW, TsaP, and TonB₂. Frontiers in Microbiology 12:738034. [PMID: 35126321]