Detailed information    

experimental Experimentally validated

Overview

Name   comO   Type   Machinery gene
Locus tag   ACIAD_RS15185 Genome accession   NC_005966
Coordinates   3265052..3265789 (-) Length   245 a.a.
NCBI ID   WP_004923719.1    Uniprot ID   A0A7G2SAI8
Organism   Acinetobacter baylyi ADP1     
Function   assembly of type IV pilus   
DNA binding and uptake

Function

The proteins ComM, ComN, ComO and ComL form the IM assembly platform of the DNA transporter which connects the energy generating cytoplasmic ATPases with the DNA transporter pseudopilus.


Genomic Context

Location: 3260052..3270789
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACIAD_RS15165 (ACIAD3353) aroB 3260650..3261738 (-) 1089 WP_004923735.1 3-dehydroquinate synthase -
  ACIAD_RS15170 (ACIAD3354) aroK 3261760..3262302 (-) 543 WP_004923733.1 shikimate kinase AroK -
  ACIAD_RS15175 (ACIAD3355) comQ 3262339..3264468 (-) 2130 WP_004923730.1 type IV pilus secretin PilQ Machinery gene
  ACIAD_RS15180 (ACIAD3356) comL 3264528..3265052 (-) 525 WP_004923726.1 pilus assembly protein PilP Machinery gene
  ACIAD_RS15185 (ACIAD3357) comO 3265052..3265789 (-) 738 WP_004923719.1 type 4a pilus biogenesis protein PilO Machinery gene
  ACIAD_RS15190 (ACIAD3359) comN 3265786..3266424 (-) 639 WP_004923716.1 PilN domain-containing protein Machinery gene
  ACIAD_RS15195 (ACIAD3360) comM 3266421..3267482 (-) 1062 WP_004923715.1 pilus assembly protein PilM Machinery gene
  ACIAD_RS15200 (ACIAD3361) ponA 3267648..3270206 (+) 2559 WP_004923711.1 penicillin-binding protein PBP1a -

Sequence

Protein


Download         Length: 245 a.a.        Molecular weight: 27921.83 Da        Isoelectric Point: 6.2680

>NTDB_id=1012 ACIAD_RS15185 WP_004923719.1 3265052..3265789(-) (comO) [Acinetobacter baylyi ADP1]
MKLDQLDKTPKESGQPKKKRMNVEKFFQQFNMLDMSNYGSWPLSVKITCWIFIFFLVCALGYFVVIRPQLDDIQTAQAQE
KNLLNEFREKQSKLRNLQQYQHQLEEMRASFNQQLTQLPKESEIPGLVEDINMTGVNSGLKFKNIRLENEVKQEFFIEQP
ISIEATGDYHAFGAFVSGIAALPRIVTLHDFVIEAKQSKEKTDIPEVDYAVKAKTYRYIGVDEQGNASAVQANTSTTTPA
QGGTP

Nucleotide


Download         Length: 738 bp        

>NTDB_id=1012 ACIAD_RS15185 WP_004923719.1 3265052..3265789(-) (comO) [Acinetobacter baylyi ADP1]
ATGAAACTTGATCAGCTTGATAAAACCCCAAAAGAATCTGGCCAACCTAAGAAAAAACGCATGAATGTTGAAAAATTCTT
TCAACAATTCAATATGCTGGATATGAGCAATTACGGCAGTTGGCCGCTCTCAGTCAAGATAACATGCTGGATTTTTATTT
TCTTTCTAGTCTGTGCGTTGGGCTACTTTGTGGTGATTCGACCTCAGCTTGACGATATTCAGACAGCGCAGGCGCAAGAA
AAAAATCTGTTAAATGAATTTCGCGAAAAACAGTCCAAACTTAGAAATTTACAACAGTACCAACATCAGCTTGAAGAAAT
GCGTGCGAGCTTTAATCAGCAGCTCACACAACTTCCCAAAGAAAGCGAAATTCCTGGATTGGTCGAAGATATTAATATGA
CAGGCGTAAATTCTGGTCTGAAATTTAAGAACATTCGTCTTGAAAATGAGGTTAAGCAGGAATTCTTTATTGAGCAACCC
ATTTCAATAGAGGCTACCGGGGATTATCATGCCTTTGGTGCCTTTGTGAGTGGTATTGCTGCATTACCTCGAATTGTGAC
CTTGCATGATTTTGTAATTGAAGCCAAACAGAGTAAAGAGAAAACCGATATTCCAGAAGTTGATTACGCTGTGAAAGCGA
AAACATATCGTTACATTGGTGTCGATGAGCAGGGCAATGCATCAGCTGTTCAGGCCAATACCTCAACGACAACTCCTGCA
CAAGGAGGTACACCATAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(73-219)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7G2SAI8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

69.421

98.776

0.686
  pilO Legionella pneumophila strain ERS1305867

39.791

95.98

0.382

Multiple sequence alignment    



References

[1] Beate Averhoff et al. (2021) Natural transformation in Gram-negative bacteria thriving in extreme environments: from genes and genomes to proteins, structures and regulation. Extremophiles : Life Under Extreme Conditions 25(5-6):425-436. [PMID: 34542714]