Detailed information NTG0001009

Experimentally validated

Overview

• Name: comQ
• Type: Machinery gene
• Locus tag: ACIAD_RS15175
• Genome accession: NC_005966
• Coordinates: 3262339..3264468 (-)
• Length: 709 a.a.
• NCBI ID: WP_004923730.1
• Uniprot ID: -
• Organism: Acinetobacter baylyi ADP1
• Function: assembly of type IV pilus DNA binding and uptake

Function

The similarities of ComQ to the members of the secretin family suggest that several ComQ monomers form a multimeric ring-like structure in the OM. This might be the portal for a DNA-transporting pilus-like shaft.

Genomic Context

Location: 3257339..3269468

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACIAD_RS15160 (ACIAD3352)	-	3259789..3260640 (-)	852	WP_004923737.1	hypothetical protein	-
ACIAD_RS15165 (ACIAD3353)	aroB	3260650..3261738 (-)	1089	WP_004923735.1	3-dehydroquinate synthase	-
ACIAD_RS15170 (ACIAD3354)	aroK	3261760..3262302 (-)	543	WP_004923733.1	shikimate kinase AroK	-
ACIAD_RS15175 (ACIAD3355)	comQ	3262339..3264468 (-)	2130	WP_004923730.1	type IV pilus secretin PilQ	Machinery gene
ACIAD_RS15180 (ACIAD3356)	comL	3264528..3265052 (-)	525	WP_004923726.1	pilus assembly protein PilP	Machinery gene
ACIAD_RS15185 (ACIAD3357)	comO	3265052..3265789 (-)	738	WP_004923719.1	type 4a pilus biogenesis protein PilO	Machinery gene
ACIAD_RS15190 (ACIAD3359)	comN	3265786..3266424 (-)	639	WP_004923716.1	PilN domain-containing protein	Machinery gene
ACIAD_RS15195 (ACIAD3360)	comM	3266421..3267482 (-)	1062	WP_004923715.1	pilus assembly protein PilM	Machinery gene

Sequence

Protein

• Length: 709 a.a.
• Molecular weight: 76671.12 Da
• Isoelectric Point: 6.3475

>NTDB_id=1009 ACIAD_RS15175 WP_004923730.1 3262339..3264468(-) (comQ) [Acinetobacter baylyi ADP1]
MKQIIRQFSIGTVAYAIMQVASAQVAITNVVPMNIPGQGTEIRVMFNGLPPQPQAYQLEQPARLILDFDKAGQKLAKSLI
PVTTNEASSIDVSSDDKRARVVVNLKDAGAFTTRVEGNVFILKINNIQSTATPVATSAPAPQQGIGNIGFQRGAQGEGLV
VIDLQGTNTPVDVQQQGSKVVVRFPGIKIPTHLARRLNTTDFATPVASIDSYNDGSNGVISIQSTGSYEYMAYQAENKLT
ISLKRPQDNVRLNSKNNQNYTGKKISLDFQDIEVRRVLQLLADFTNINMVTADSVQGNITLHLKDVPWDQALDIILKTKN
LDKRRNGNVIWIAPVSELIKSEEDEAKAIAQSTKLAPLQTEYILLKYAKAADIEKLITQGRNSGTSNSNTNGSATVEPLG
DSVGTLLSPRGTISLDPRTNTLIINDTSQKIDQIRKMIDLLDIPVKQVMIEARIVRATTSFSKEMGVKWGILSQGINQNS
NLLVGGSETTLWNLRNPDSNGTYTIERPDNLNVDLGVTTTGASSIAFGLISLSDFMLDLELSALQADGYGEVISTPKVMS
ADKQKAKVATGVEVPYQSTTNSAAGSTATTSFKEALLSLEVTPNITPDGKILMELNIANDSINSYAQNGEAILNKNTINT
NVLVNNGETVVLGGVFEQTTNNAVTKVPFLGDLPYVGRLFKKTAKSEAKNELLIFVTPRIVNDTIVGNH

Nucleotide

• Length: 2130 bp

>NTDB_id=1009 ACIAD_RS15175 WP_004923730.1 3262339..3264468(-) (comQ) [Acinetobacter baylyi ADP1]
ATGAAACAGATCATACGTCAATTTTCGATCGGGACAGTTGCTTATGCCATCATGCAGGTTGCAAGTGCTCAGGTTGCAAT
CACTAACGTGGTTCCAATGAATATTCCAGGACAAGGCACAGAAATTCGGGTCATGTTTAATGGATTGCCGCCTCAGCCTC
AAGCCTATCAACTTGAGCAGCCAGCGCGTCTGATTCTGGATTTTGATAAAGCAGGTCAGAAGCTTGCAAAAAGCTTGATT
CCTGTGACCACTAATGAAGCTAGCTCAATTGATGTTAGTTCGGATGATAAACGCGCCAGAGTTGTGGTTAATCTAAAAGA
TGCTGGCGCATTCACCACACGCGTTGAAGGCAATGTTTTCATTCTTAAAATTAACAATATTCAATCGACTGCAACGCCTG
TTGCAACGTCAGCACCTGCGCCTCAGCAGGGCATTGGTAATATCGGTTTTCAGCGTGGTGCTCAAGGTGAAGGGCTGGTT
GTTATTGATTTGCAAGGAACAAATACGCCTGTTGATGTGCAGCAACAAGGCAGCAAAGTGGTGGTGCGTTTCCCAGGAAT
CAAAATACCCACACATTTAGCTCGTCGTCTAAATACAACTGATTTTGCAACACCTGTTGCTTCAATAGATTCCTATAATG
ATGGCAGTAATGGGGTTATTTCCATTCAGTCTACGGGTAGCTACGAATATATGGCCTATCAGGCTGAAAATAAGCTCACC
ATTAGTTTAAAACGTCCGCAGGATAATGTACGCCTCAATTCCAAAAACAATCAGAATTATACAGGGAAAAAGATTTCTCT
AGATTTTCAGGATATTGAAGTGCGCCGTGTACTACAGCTACTTGCAGACTTCACCAATATTAATATGGTCACTGCCGATT
CTGTACAGGGTAATATTACGTTGCATCTTAAAGATGTACCTTGGGATCAGGCACTGGATATTATTTTAAAAACAAAAAAT
CTGGATAAACGCCGTAATGGGAATGTGATCTGGATTGCGCCTGTTTCAGAGTTGATTAAATCTGAAGAAGATGAAGCGAA
GGCAATTGCGCAAAGTACCAAGCTTGCACCGCTACAAACTGAATATATTTTATTGAAATATGCTAAAGCTGCAGATATTG
AAAAGTTAATTACCCAAGGGCGTAATAGTGGTACATCCAATAGCAATACGAATGGCAGTGCGACTGTTGAACCTTTAGGC
GATAGTGTTGGAACTTTGCTGAGTCCAAGAGGAACCATTTCCCTTGATCCGCGAACCAATACCTTAATTATTAATGATAC
CTCACAAAAAATTGACCAGATTCGTAAGATGATTGACTTACTGGATATTCCAGTCAAGCAGGTGATGATTGAAGCACGGA
TCGTACGTGCGACGACATCTTTTAGTAAAGAAATGGGTGTGAAATGGGGGATTTTATCTCAAGGTATCAATCAAAATAGC
AATCTGTTAGTGGGCGGGAGTGAAACAACGCTTTGGAACTTGCGTAACCCTGATAGTAACGGGACGTATACGATAGAACG
TCCAGACAATCTGAATGTGGATCTTGGTGTGACCACCACAGGTGCAAGCAGTATTGCCTTTGGTTTGATTAGTCTTTCTG
ATTTTATGCTGGATTTAGAACTTTCTGCGTTGCAGGCAGATGGATATGGTGAAGTCATTTCGACACCTAAAGTCATGTCG
GCAGATAAGCAAAAGGCTAAAGTTGCAACCGGTGTTGAAGTTCCGTATCAATCAACAACCAATTCGGCTGCTGGTTCAAC
GGCGACAACATCATTTAAAGAAGCATTACTCAGCCTTGAAGTAACACCTAATATTACCCCAGACGGCAAAATCTTGATGG
AACTCAACATTGCCAACGACTCAATTAACAGTTATGCCCAAAATGGCGAAGCGATTTTAAATAAAAATACCATTAACACC
AATGTGCTGGTGAATAATGGCGAAACTGTAGTATTGGGTGGCGTATTTGAGCAAACAACCAACAATGCCGTGACTAAAGT
ACCATTTTTAGGTGATCTTCCTTACGTTGGACGCCTATTTAAGAAGACGGCCAAAAGTGAGGCAAAAAATGAGCTGCTAA
TTTTTGTGACGCCTCGAATAGTTAATGACACTATTGTTGGAAATCATTAA

Domains

Predicted by InterproScan.

• Secretin and TonB N terminus short domain (STN) (291-334)

• Bacterial type II/III secretion system short domain (Secretin_N) (362-447)

• Bacterial type II and III secretion system protein (Secretin) (543-701)

• AMIN domain (AMIN) (40-111)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilQ	Acinetobacter baumannii D1279779	75.241	100	0.772
pilQ	Acinetobacter baumannii strain A118	75.103	100	0.77
pilQ	Pseudomonas aeruginosa PAK	40.883	99.013	0.405
pilQ	Vibrio cholerae strain A1552	39.336	100	0.394
pilQ	Vibrio cholerae O1 biovar El Tor strain E7946	39.336	100	0.394
pilQ	Legionella pneumophila strain ERS1305867	38.719	98.424	0.381
pilQ	Legionella pneumophila strain Lp02	38.428	98.424	0.378
pilQ	Vibrio campbellii strain DS40M4	36.788	99.828	0.367

References

• [1]: Beate Averhoff et al. (2021) Natural transformation in Gram-negative bacteria thriving in extreme environments: from genes and genomes to proteins, structures and regulation. Extremophiles : Life Under Extreme Conditions 25(5-6):425-436. [PMID: 34542714]
• [2]: Colleen G Leong et al. (2017) The role of core and accessory type IV pilus genes in natural transformation and twitching motility in the bacterium Acinetobacter baylyi. PloS One 12(8):e0182139. [PMID: 28771515]