Detailed information NTG0001193

Experimentally validated

Overview

• Name: pilQ
• Type: Machinery gene
• Locus tag: PAKAF_RS26665
• Genome accession: NZ_LR657304
• Coordinates: 5798059..5800203 (-)
• Length: 714 a.a.
• NCBI ID: WP_003161249.1
• Uniprot ID: A0A8G2V818
• Organism: Pseudomonas aeruginosa PAK
• Function: assembly of type IV pilus DNA binding and uptake

Function

P. aeruginosa in biofilms is competent for natural transformation of both genomic and plasmid DNA. All T4P mutants were capable of some natural transformation of pUCPSK, however a significant reduction in transformation frequency compared to wild-type PAK was observed.

Genomic Context

Location: 5793059..5805203

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PAKAF_RS26650 (PAKAF_05353)	-	5794711..5796366 (-)	1656	WP_016253982.1	AAA family ATPase	-
PAKAF_RS26655 (PAKAF_05354)	aroB	5796373..5797479 (-)	1107	WP_016253983.1	3-dehydroquinate synthase	-
PAKAF_RS26660 (PAKAF_05355)	aroK	5797529..5798047 (-)	519	WP_003095833.1	shikimate kinase AroK	-
PAKAF_RS26665 (PAKAF_05356)	pilQ	5798059..5800203 (-)	2145	WP_003161249.1	type 4a pilus secretin PilQ	Machinery gene
PAKAF_RS26670 (PAKAF_05357)	pilP	5800257..5800781 (-)	525	WP_003095836.1	type 4a pilus biogenesis lipoprotein PilP	-
PAKAF_RS26675 (PAKAF_05358)	pilO	5800778..5801401 (-)	624	WP_003103268.1	type 4a pilus biogenesis protein PilO	-
PAKAF_RS26680 (PAKAF_05359)	pilN	5801398..5801994 (-)	597	WP_003095839.1	type 4a pilus biogenesis protein PilN	-
PAKAF_RS26685 (PAKAF_05360)	pilM	5801994..5803058 (-)	1065	WP_003110888.1	type IV pilus assembly protein PilM	Machinery gene

Sequence

Protein

• Length: 714 a.a.
• Molecular weight: 77438.99 Da
• Isoelectric Point: 5.2285

>NTDB_id=1193 PAKAF_RS26665 WP_003161249.1 5798059..5800203(-) (pilQ) [Pseudomonas aeruginosa PAK]
MNSGLSRLGIALLAAMFAPALLAADLEKLDVAALPGDRVELKLQFDEPVAAPRGYTIEQPARIALDLPGVQNKLGTKNRE
LSVGNTRSVTVVEAKDRTRLIINLTTLSSYTTRVEGNNLFVVVGNSPAGASVASTAPVKASPAPASYAQPIKPKPYVPAG
RAIRNIDFQRGEKGEGNVVIDLSDPTLSPDIQEQGGKIRLDFAKTQLPDALRVRLDVKDFATPVQFVNASAQSDRTSITI
EPSGLYDYLVYQTDNRLTVSIKPMTTEDAERRKKDNFAYTGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVQGNITL
RLQNVPWDQALDLVLKTKGLDKRKLGNVLLVAPADEIAARERQELEAQKQIAELAPLRRELIQVNYAKAADIAKLFQSVT
SDGGQEGKEGGRGSITVDDRTNSIIAYQPQERLDELRRIVSQLDIPVRQVMIEARIVEANVGYDKSLGVRWGGAYHKGNW
SGYGKDGNIGIKDEDGMNCGPIAGSCTFPTTGTSKSPSPFVDLGAKDATSGIGIGFITDNIILDLQLSAMEKTGNGEIVS
QPKVVTSDKETAKILKGSEVPYQEASSSGATSTSFKEAALSLEVTPQITPDNRIIVEVKVTKDAPDYQNMLNGVPPINKN
EVNAKILVNDGETIVIGGVFSNEQSKSVEKVPFLGELPYLGRLFRRDTVTDRKNELLVFLTPRIMNNQAIAIGR

Nucleotide

• Length: 2145 bp

>NTDB_id=1193 PAKAF_RS26665 WP_003161249.1 5798059..5800203(-) (pilQ) [Pseudomonas aeruginosa PAK]
ATGAACAGTGGCCTCTCGCGCCTCGGGATCGCTTTGCTGGCCGCCATGTTCGCGCCGGCATTGCTCGCCGCGGACCTGGA
GAAACTCGACGTGGCGGCACTGCCTGGCGACCGGGTCGAGCTAAAGCTGCAGTTCGACGAGCCCGTCGCCGCGCCGCGTG
GCTATACCATCGAGCAGCCGGCGCGAATCGCCCTCGATCTGCCGGGGGTGCAGAACAAACTCGGCACCAAGAATCGCGAG
CTGAGCGTGGGCAATACGCGCAGCGTCACGGTGGTCGAGGCGAAGGATCGCACACGGTTGATCATCAACCTGACCACGTT
GTCGTCCTACACCACCCGGGTGGAAGGCAACAACCTCTTCGTGGTGGTCGGCAATTCGCCGGCCGGCGCCAGCGTCGCTT
CCACCGCTCCGGTCAAGGCTAGCCCGGCTCCGGCCAGCTATGCCCAGCCGATCAAGCCCAAGCCATACGTGCCGGCGGGT
CGTGCCATTCGCAACATCGATTTCCAGCGTGGCGAAAAGGGCGAGGGCAACGTGGTCATCGACCTGTCCGACCCGACCTT
GAGCCCGGATATCCAGGAGCAGGGTGGCAAGATCCGCCTGGACTTCGCCAAGACCCAGTTGCCGGATGCGCTGCGGGTTC
GCCTGGACGTCAAGGACTTCGCCACGCCGGTGCAGTTCGTCAACGCCAGCGCGCAATCCGACCGTACCAGCATCACCATC
GAGCCGAGCGGGCTCTACGACTATCTGGTCTACCAGACCGACAATCGCCTGACCGTCAGCATCAAGCCGATGACCACGGA
AGACGCCGAGCGGCGCAAGAAGGACAATTTCGCCTATACCGGCGAGAAACTGTCGCTGAACTTCCAGGACATCGACGTGC
GTTCGGTACTGCAGCTGATCGCCGACTTCACCGACCTGAACCTGGTGGCCTCGGATACCGTGCAGGGCAATATCACCCTG
CGCCTGCAGAACGTGCCGTGGGACCAGGCGCTGGACCTGGTGCTGAAGACCAAGGGCCTGGACAAGCGCAAGCTCGGCAA
CGTGCTCCTGGTGGCCCCGGCCGACGAGATCGCCGCCCGTGAGCGCCAGGAGCTGGAAGCGCAGAAGCAGATCGCCGAGT
TGGCGCCACTGCGTCGGGAACTGATCCAGGTGAACTACGCCAAGGCCGCGGACATCGCCAAGCTGTTCCAGTCGGTGACC
AGCGATGGTGGCCAGGAAGGCAAGGAAGGCGGGCGCGGCTCGATCACCGTCGACGACCGCACCAACAGCATCATCGCCTA
TCAGCCCCAGGAGCGACTGGACGAACTGCGGCGGATCGTTTCGCAGCTGGATATTCCGGTGCGCCAGGTGATGATCGAGG
CACGCATCGTCGAGGCCAACGTCGGTTACGACAAGAGTCTCGGTGTGCGCTGGGGCGGCGCTTATCACAAGGGCAACTGG
AGCGGCTACGGCAAGGACGGCAATATCGGGATCAAGGACGAGGACGGCATGAACTGCGGCCCGATCGCCGGTAGCTGTAC
CTTCCCGACCACGGGTACCAGCAAGTCGCCGTCGCCGTTCGTCGACCTCGGTGCCAAGGATGCTACCTCCGGGATCGGCA
TCGGCTTCATCACCGACAACATCATCCTCGACCTGCAACTGTCGGCGATGGAAAAGACCGGCAATGGCGAGATCGTCTCC
CAGCCCAAGGTCGTGACCTCGGACAAGGAAACCGCGAAGATCCTGAAGGGCTCGGAAGTCCCCTACCAGGAGGCCAGTTC
CAGCGGCGCTACCAGCACCTCGTTCAAGGAAGCCGCGCTGTCCCTCGAGGTGACCCCGCAGATCACCCCGGACAACCGGA
TCATCGTCGAAGTCAAGGTGACCAAGGACGCGCCGGATTACCAGAATATGTTGAACGGCGTACCTCCTATCAACAAGAAC
GAGGTCAACGCCAAGATCCTGGTCAACGACGGCGAGACCATCGTCATCGGCGGCGTGTTCAGTAACGAGCAGAGCAAGTC
GGTGGAGAAGGTACCCTTCCTCGGCGAACTGCCTTACCTCGGGCGGCTGTTCCGGCGAGATACCGTCACCGACCGGAAAA
ACGAACTTCTGGTTTTCCTGACACCGCGGATCATGAATAATCAGGCCATCGCAATCGGTCGCTGA

Domains

Predicted by InterproScan.

• Bacterial type II/III secretion system short domain (Secretin_N) (381-449)

• Secretin and TonB N terminus short domain (STN) (307-353)

• AMIN domain (AMIN) (38-122)

• Bacterial type II and III secretion system protein (Secretin) (549-705)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilQ	Acinetobacter baumannii D1279779	40.699	100	0.408
pilQ	Acinetobacter baumannii strain A118	40.699	100	0.408
comQ	Acinetobacter baylyi ADP1	40.883	99.013	0.405
pilQ	Vibrio campbellii strain DS40M4	40.354	97.414	0.393
pilQ	Legionella pneumophila strain ERS1305867	37.73	100	0.381
pilQ	Legionella pneumophila strain Lp02	37.73	100	0.381
pilQ	Vibrio cholerae O1 biovar El Tor strain E7946	39.344	96.147	0.378
pilQ	Vibrio cholerae strain A1552	39.344	96.147	0.378

References

• [1]: Laura M Nolan et al. (2020) Pseudomonas aeruginosa is capable of natural transformation in biofilms. Microbiology (Reading, England) 166(10):995-1003. [PMID: 32749953]