Detailed information NTG0001052

Experimentally validated

Overview

• Name: pilQ
• Type: Machinery gene
• Locus tag: ABD1_RS15850
• Genome accession: NC_020547
• Coordinates: 3349027..3351192 (-)
• Length: 721 a.a.
• NCBI ID: WP_001017033.1
• Uniprot ID: -
• Organism: Acinetobacter baumannii D1279779
• Function: assembly of type IV pilus DNA binding and uptake

Function

We examined the 33 knockout mutants by comparing their transformation efficiency with wild-type W068. No knockout strains were affected in growth, but 28 of these mutants were severely or completely impaired in natural transformability. These mutations were in 18 genes related to T4P (pilF, pilQ, tsaP, pilM, pilN, pilO, pilP, pilB, pilC, pilT, pilD, pilE, pilY2, pilY1, pilX, pilW, pilV, and fimU), six genes related to DNA uptake and processing (comEA, comA, comF, priA, dprA, and recA), two genes related to T2SS (xcpV and xcpW), and the crp and tonB2 genes.

Genomic Context

Location: 3344027..3356192

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ABD1_RS15835 (ABD1_30730)	-	3346444..3347319 (-)	876	WP_000218807.1	hypothetical protein	-
ABD1_RS15840 (ABD1_30740)	aroB	3347335..3348417 (-)	1083	WP_001193999.1	3-dehydroquinate synthase	-
ABD1_RS15845 (ABD1_30750)	aroK	3348446..3349015 (-)	570	WP_000643712.1	shikimate kinase AroK	-
ABD1_RS15850 (ABD1_30760)	pilQ	3349027..3351192 (-)	2166	WP_001017033.1	type IV pilus secretin PilQ family protein	Machinery gene
ABD1_RS15855 (ABD1_30770)	pilP	3351255..3351782 (-)	528	WP_000695065.1	pilus assembly protein PilP	Machinery gene
ABD1_RS15860 (ABD1_30780)	pilO	3351793..3352533 (-)	741	WP_000076101.1	type 4a pilus biogenesis protein PilO	Machinery gene
ABD1_RS15865 (ABD1_30790)	pilN	3352530..3353171 (-)	642	WP_000201227.1	PilN domain-containing protein	Machinery gene
ABD1_RS15870 (ABD1_30800)	pilM	3353171..3354229 (-)	1059	WP_002014066.1	pilus assembly protein PilM	Machinery gene

Sequence

Protein

• Length: 721 a.a.
• Molecular weight: 77891.28 Da
• Isoelectric Point: 6.5404

>NTDB_id=1052 ABD1_RS15850 WP_001017033.1 3349027..3351192(-) (pilQ) [Acinetobacter baumannii D1279779]
MNHVFRQFSMGAVAIAIMQAASAQVSMTNIVPMQIAGQGTEIRVMFNGLPPQPQAYQLENPSRLILDFDKAQQGLKQSKI
SVATNEASSVDVTSDDQRSRLTVNLKDAGAFTTRVEGNTFILKINSAQTSNKPLPVVSAQPQGVSNIGFQRGSQGEGLVV
VDLLGSNTPVDVQQQGSKVVIRTIGTKIPTHLARRLNVNDFATPVSSIDAYNDKGVGVITIQSSGSYEYMAYQAENKLTI
SLKRPQDKNVTSLYKTPNYSGNKLSLDFQDIEVRRVLQLLADFTGINMVAADSVQGNITLRLKDVPWDQALDIILKTKNL
DKRRNGNVIWIAPVAELIKAEEEEAKAVAQSVKLAPLQTEYIQLKYAKAGDIMGLITQGSNNSNGLQHTSGGGTSTSTNL
NTGVDSLGNNVGSLLSPRGTITQDDRTNTLIINDTAQSIDQIRKMIDLLDVQVKQVMVEARIVRASTSFTKELGVKWGIL
SQGITNNNNLLVGGSETTLWNLREPKKDETTGGYKYTIERPDNLNVDLGVSNPAGSIAFGLISMSDFMLDLELSALQADG
YGEVISTPKVMTADKQTAKVATGQQVPYQSTTNSAAGSTATTSFKDALLSLNVTPSITPDGKIQMKLDISKDSVAGAAPN
GELILNKNNINTNVLVDNGETVILGGVFEQTTMNSQTKVPFFGDIPVVGRLFRKDVKSDDKQELLIFVTPRIVNDTLARN
H

Nucleotide

• Length: 2166 bp

>NTDB_id=1052 ABD1_RS15850 WP_001017033.1 3349027..3351192(-) (pilQ) [Acinetobacter baumannii D1279779]
ATGAATCACGTATTCCGTCAGTTTTCTATGGGGGCTGTAGCTATTGCGATTATGCAGGCTGCATCTGCACAGGTCAGCAT
GACCAACATTGTACCAATGCAAATTGCAGGTCAGGGCACAGAAATTAGAGTCATGTTTAATGGCTTGCCACCACAACCAC
AAGCTTACCAACTTGAAAATCCTTCACGTTTAATCCTTGATTTTGATAAGGCACAGCAAGGTTTAAAACAGTCTAAAATT
AGTGTTGCAACTAATGAGGCAAGTTCTGTAGATGTTACTTCAGATGACCAGCGCTCACGTTTAACTGTAAACCTAAAAGA
TGCTGGAGCATTTACAACACGAGTCGAAGGCAATACTTTCATTTTAAAAATTAATTCTGCTCAAACAAGTAATAAGCCGC
TACCTGTGGTCTCAGCACAACCACAGGGAGTTTCAAATATTGGCTTTCAGCGTGGTAGTCAGGGAGAAGGCCTGGTTGTT
GTAGATCTTTTAGGAAGTAATACGCCTGTAGATGTTCAGCAGCAGGGTAGTAAAGTCGTTATAAGAACAATAGGTACAAA
AATCCCGACACATTTAGCACGTCGCTTAAATGTAAATGATTTTGCAACCCCCGTTTCAAGTATCGATGCTTATAATGATA
AAGGGGTTGGTGTAATAACTATCCAGTCCTCTGGTAGCTATGAATATATGGCCTATCAGGCGGAGAATAAGCTCACGATC
AGTTTGAAACGTCCCCAAGATAAAAATGTTACCTCTTTATATAAAACGCCAAATTATTCAGGAAATAAACTTTCATTAGA
CTTCCAAGATATTGAAGTACGCCGTGTGTTGCAATTACTTGCTGATTTTACAGGCATCAATATGGTAGCTGCCGATAGTG
TACAAGGTAATATCACACTACGTTTAAAAGATGTACCTTGGGATCAGGCTCTTGATATTATTTTAAAAACTAAAAATCTT
GATAAACGCCGTAATGGCAATGTGATTTGGATTGCTCCAGTTGCAGAATTGATTAAAGCGGAAGAAGAAGAAGCGAAAGC
TGTTGCACAAAGTGTCAAACTTGCACCCTTACAGACTGAGTATATTCAACTGAAGTATGCTAAAGCTGGCGATATTATGG
GTCTCATTACACAAGGCTCAAATAATAGTAATGGCTTACAACATACTTCTGGTGGAGGGACCTCAACTTCAACCAATTTA
AATACTGGAGTTGATAGTTTAGGCAATAATGTGGGTAGTTTGCTTAGCCCGCGTGGCACTATTACTCAAGATGACCGAAC
TAATACTCTTATTATTAACGATACTGCTCAGTCAATTGATCAAATTCGTAAAATGATTGACTTGCTTGATGTGCAAGTTA
AACAAGTTATGGTTGAAGCACGTATTGTGAGAGCCTCTACGTCATTTACCAAAGAACTTGGTGTGAAGTGGGGTATTCTG
TCGCAAGGTATTACCAATAACAACAATTTGCTGGTTGGCGGTAGTGAAACAACTTTATGGAATTTACGCGAACCTAAAAA
AGATGAAACTACAGGTGGGTATAAATATACGATTGAGCGCCCAGATAACTTAAACGTGGATTTAGGAGTTTCTAATCCTG
CAGGAAGTATTGCTTTTGGCTTGATTAGTATGTCTGACTTTATGTTAGATCTTGAATTGTCTGCTTTACAAGCGGATGGT
TATGGTGAGGTTATTTCTACCCCTAAAGTCATGACTGCCGATAAACAAACAGCGAAAGTTGCAACTGGTCAACAAGTTCC
ATATCAATCTACAACTAACTCTGCAGCAGGTTCAACTGCTACAACATCTTTTAAAGATGCTTTGCTGAGTTTAAATGTGA
CACCAAGTATTACACCTGATGGCAAGATTCAAATGAAGTTGGATATTTCTAAGGATTCTGTAGCGGGTGCGGCTCCAAAT
GGAGAGTTGATTTTAAATAAAAATAATATCAACACCAATGTATTGGTAGATAATGGCGAAACTGTCATTTTGGGTGGTGT
TTTTGAGCAAACGACTATGAATTCACAAACCAAAGTTCCTTTCTTTGGTGATATTCCTGTTGTTGGGCGTTTATTTAGAA
AAGATGTAAAGTCTGATGATAAACAAGAGTTGCTGATTTTCGTTACACCACGAATTGTTAATGACACTTTAGCGAGAAAT
CATTAA

Domains

Predicted by InterproScan.

• Secretin and TonB N terminus short domain (STN) (291-333)

• Bacterial type II/III secretion system short domain (Secretin_N) (360-455)

• AMIN domain (AMIN) (39-121)

• Bacterial type II and III secretion system protein (Secretin) (555-713)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilQ	Acinetobacter baumannii strain A118	99.861	100	0.999
comQ	Acinetobacter baylyi ADP1	75.241	100	0.772
pilQ	Pseudomonas aeruginosa PAK	40.699	100	0.408
pilQ	Vibrio cholerae strain A1552	37.819	100	0.389
pilQ	Vibrio cholerae O1 biovar El Tor strain E7946	37.819	100	0.389
pilQ	Vibrio campbellii strain DS40M4	37.521	100	0.386
pilQ	Legionella pneumophila strain Lp02	35.095	100	0.371

References

• [1]: Yuan Hu et al. (2021) Natural Transformation in Acinetobacter baumannii W068: A Genetic Analysis Reveals the Involvements of the CRP, XcpV, XcpW, TsaP, and TonB₂. Frontiers in Microbiology 12:738034. [PMID: 35126321]