Detailed information    

experimental Experimentally validated

Overview

Name   comM   Type   Machinery gene
Locus tag   ACIAD_RS15195 Genome accession   NC_005966
Coordinates   3266421..3267482 (-) Length   353 a.a.
NCBI ID   WP_004923715.1    Uniprot ID   A0A7G2SBG5
Organism   Acinetobacter baylyi ADP1     
Function   assembly of type IV pilus   
DNA binding and uptake

Function

The proteins ComM, ComN, ComO and ComL form the IM assembly platform of the DNA transporter which connects the energy generating cytoplasmic ATPases with the DNA transporter pseudopilus.


Genomic Context

Location: 3261421..3272482
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACIAD_RS15170 (ACIAD3354) aroK 3261760..3262302 (-) 543 WP_004923733.1 shikimate kinase AroK -
  ACIAD_RS15175 (ACIAD3355) comQ 3262339..3264468 (-) 2130 WP_004923730.1 type IV pilus secretin PilQ Machinery gene
  ACIAD_RS15180 (ACIAD3356) comL 3264528..3265052 (-) 525 WP_004923726.1 pilus assembly protein PilP Machinery gene
  ACIAD_RS15185 (ACIAD3357) comO 3265052..3265789 (-) 738 WP_004923719.1 type 4a pilus biogenesis protein PilO Machinery gene
  ACIAD_RS15190 (ACIAD3359) comN 3265786..3266424 (-) 639 WP_004923716.1 PilN domain-containing protein Machinery gene
  ACIAD_RS15195 (ACIAD3360) comM 3266421..3267482 (-) 1062 WP_004923715.1 pilus assembly protein PilM Machinery gene
  ACIAD_RS15200 (ACIAD3361) ponA 3267648..3270206 (+) 2559 WP_004923711.1 penicillin-binding protein PBP1a -
  ACIAD_RS15205 (ACIAD3362) - 3270219..3271040 (-) 822 WP_011182767.1 putative RNA methyltransferase -
  ACIAD_RS15210 (ACIAD3363) mraY 3271106..3272224 (-) 1119 WP_004923704.1 phospho-N-acetylmuramoyl-pentapeptide- transferase -

Sequence

Protein


Download         Length: 353 a.a.        Molecular weight: 39258.99 Da        Isoelectric Point: 4.6097

>NTDB_id=1010 ACIAD_RS15195 WP_004923715.1 3266421..3267482(-) (comM) [Acinetobacter baylyi ADP1]
MLRLYRRPKKGLVGVDISSTSVKVLELSVKNNQYRVESYGLVPLLENSVVEKNILNPEAVADALTRSINLANPQAINAAI
AVPTSMVVHKIIEMDADMTDDEREVQIRMDAERYIPFPLDEVSLDFEVMSEPLANPNRVSVLIAATRTENVDTRIEVLEL
AGLTPKVADVESYAMERAFSVFADTLPMGVNTVGILDIGHTMTTLSVMQKGKIIYTREQVFGGKQLTQEVQKRYGMSYEE
AGRAKKDGSLPEDFTQEVFEPYLEALVQQAARSLQFFFSSSQYNEIDHILLAGGNANILGLSRLLQQKLGYRVTIANPFL
QMEFSPQVDIKKVENDAASLMVACGLALRSFDE

Nucleotide


Download         Length: 1062 bp        

>NTDB_id=1010 ACIAD_RS15195 WP_004923715.1 3266421..3267482(-) (comM) [Acinetobacter baylyi ADP1]
GTGCTCAGGTTATATCGTAGGCCAAAGAAGGGGTTAGTGGGAGTCGATATTAGTTCGACTTCTGTTAAAGTATTAGAGCT
ATCTGTAAAGAATAATCAGTATCGTGTTGAGAGCTATGGTCTGGTGCCGTTGCTTGAAAATAGTGTTGTAGAAAAAAATA
TTCTCAATCCAGAAGCGGTTGCAGATGCACTCACGCGTTCAATCAATCTGGCCAATCCCCAAGCTATCAATGCAGCGATT
GCTGTTCCTACTTCTATGGTCGTACACAAGATTATAGAGATGGATGCAGATATGACCGATGATGAAAGGGAAGTTCAGAT
CCGTATGGATGCTGAGCGTTACATTCCCTTTCCTCTGGATGAGGTCAGCCTAGATTTTGAAGTGATGTCAGAGCCTCTTG
CCAATCCTAATCGCGTGAGTGTTTTAATTGCTGCAACCCGTACAGAAAATGTAGATACACGTATTGAGGTGCTTGAGCTG
GCAGGGTTGACACCAAAGGTCGCAGATGTGGAAAGCTATGCCATGGAACGCGCATTTAGCGTATTTGCAGATACGCTGCC
TATGGGCGTAAATACCGTCGGTATTTTGGATATTGGACATACCATGACCACCCTGTCTGTGATGCAAAAAGGAAAAATCA
TCTATACGCGTGAGCAGGTTTTTGGTGGTAAGCAGCTTACTCAGGAAGTTCAAAAACGCTATGGCATGTCTTATGAGGAA
GCTGGACGTGCGAAGAAGGACGGTTCTTTACCTGAAGATTTCACTCAGGAAGTTTTTGAGCCTTATTTGGAAGCCTTGGT
CCAGCAAGCGGCACGTTCTTTGCAGTTTTTCTTTTCATCTTCGCAATACAATGAAATTGACCATATTTTATTGGCAGGAG
GCAATGCAAACATTCTAGGATTATCTCGGTTATTACAGCAAAAGCTTGGTTATCGGGTGACGATTGCCAATCCATTTTTA
CAAATGGAGTTTTCGCCTCAGGTCGATATTAAGAAAGTCGAAAATGATGCTGCTTCACTCATGGTCGCTTGTGGTCTGGC
TCTGAGGAGTTTTGATGAATGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-351)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7G2SBG5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

81.534

100

0.815
  comM Acinetobacter nosocomialis M2

81.25

100

0.813
  pilM Legionella pneumophila strain ERS1305867

40.678

100

0.408

Multiple sequence alignment    



References

[1] Beate Averhoff et al. (2021) Natural transformation in Gram-negative bacteria thriving in extreme environments: from genes and genomes to proteins, structures and regulation. Extremophiles : Life Under Extreme Conditions 25(5-6):425-436. [PMID: 34542714]
[2] Thomas M Nero et al. (2018) ComM is a hexameric helicase that promotes branch migration during natural transformation in diverse Gram-negative species. Nucleic Acids Research 46(12):6099-6111. [PMID: 29722872]
[3] Colleen G Leong et al. (2017) The role of core and accessory type IV pilus genes in natural transformation and twitching motility in the bacterium Acinetobacter baylyi. PloS One 12(8):e0182139. [PMID: 28771515]