Detailed information    

experimental Experimentally validated

Overview

Name   pilO   Type   Machinery gene
Locus tag   GCO85_RS04845 Genome accession   NZ_CP048618
Coordinates   1055645..1056244 (+) Length   199 a.a.
NCBI ID   WP_011213355.1    Uniprot ID   -
Organism   Legionella pneumophila strain ERS1305867     
Function   assembly of type IV pilus   
DNA binding and uptake

Function

Mutation of this gene caused a decrease in transformation.


Genomic Context

Location: 1050645..1061244
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GCO85_RS04830 (GCO85_04840) ravI 1052591..1053598 (-) 1008 WP_011946020.1 Dot/Icm T4SS effector RavI -
  GCO85_RS04835 (GCO85_04845) pilM 1054015..1055079 (+) 1065 WP_011215094.1 type IV pilus assembly protein PilM Machinery gene
  GCO85_RS04840 (GCO85_04850) pilN 1055083..1055631 (+) 549 WP_011213354.1 PilN domain-containing protein Machinery gene
  GCO85_RS04845 (GCO85_04855) pilO 1055645..1056244 (+) 600 WP_011213355.1 type 4a pilus biogenesis protein PilO Machinery gene
  GCO85_RS04850 (GCO85_04860) pilP 1056241..1056825 (+) 585 WP_011213356.1 pilus assembly protein PilP Machinery gene
  GCO85_RS04855 (GCO85_04865) pilQ 1056829..1058925 (+) 2097 WP_163621000.1 type IV pilus secretin PilQ Machinery gene
  GCO85_RS04860 (GCO85_04870) aroK 1059378..1059899 (+) 522 WP_027219629.1 shikimate kinase AroK -
  GCO85_RS04865 (GCO85_04875) aroB 1059886..1060995 (+) 1110 WP_013101269.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 199 a.a.        Molecular weight: 22523.24 Da        Isoelectric Point: 5.3369

>NTDB_id=1255 GCO85_RS04845 WP_011213355.1 1055645..1056244(+) (pilO) [Legionella pneumophila strain ERS1305867]
MDTVNLNELTLENVGQWPMPVKIGVILGISILIIGLGYWLIVKPNFEQLEIQEAQEIALKTEFEKKQHQAANLQAYRNQL
QLMNERFGTMLKQLPAKNEMPGLLEEISKTGIASGLKFELFAPQPEVVHDFYVELPIKITIVGSYMQLARFLSRVAEMNR
IVTLHEFSVEGVSSGDGKTVSQDELVMAITAKIYRYRAQ

Nucleotide


Download         Length: 600 bp        

>NTDB_id=1255 GCO85_RS04845 WP_011213355.1 1055645..1056244(+) (pilO) [Legionella pneumophila strain ERS1305867]
ATGGACACAGTCAATTTAAATGAATTAACTCTGGAGAATGTCGGGCAGTGGCCAATGCCGGTTAAAATCGGAGTAATTTT
AGGCATCAGTATACTGATAATTGGATTAGGATATTGGCTTATTGTTAAGCCCAATTTTGAGCAATTGGAAATACAGGAAG
CCCAGGAAATTGCTTTGAAAACAGAGTTTGAAAAAAAGCAACATCAGGCAGCAAATCTACAAGCTTATCGTAATCAGTTA
CAGCTAATGAATGAACGCTTTGGGACTATGCTAAAACAACTCCCGGCGAAAAATGAAATGCCTGGGCTTTTGGAGGAAAT
ATCTAAAACGGGGATCGCATCGGGTTTGAAATTTGAATTGTTTGCACCACAACCTGAAGTAGTGCATGATTTTTATGTGG
AGTTACCTATAAAAATTACGATTGTCGGATCCTATATGCAATTGGCAAGATTTTTAAGTCGTGTAGCTGAAATGAACAGA
ATAGTGACCTTGCATGAATTTAGTGTTGAGGGGGTATCATCCGGGGATGGTAAAACAGTCTCTCAGGATGAATTGGTGAT
GGCTATTACTGCGAAAATATACAGATATCGGGCACAATGA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(49-197)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comO Acinetobacter baylyi ADP1

39.791

95.98

0.382
  pilO Acinetobacter baumannii D1279779

38.22

95.98

0.367

Multiple sequence alignment    



References

[1] Léo Hardy et al. (2021) Transposon Insertion Sequencing in a Clinical Isolate of Legionella pneumophila Identifies Essential Genes and Determinants of Natural Transformation. Journal of Bacteriology 203(3):e00548-20. [PMID: 33168636]