Detailed information    

experimental Experimentally validated

Overview

Name   pilS   Type   Regulator
Locus tag   H0N27_RS16325 Genome accession   NZ_CP059039
Coordinates   3493513..3495081 (+) Length   522 a.a.
NCBI ID   WP_206677389.1    Uniprot ID   -
Organism   Acinetobacter baumannii strain A118     
Function   regulation of type IV pilus assembly   
Competence regulation

Function

In contrast to A. baylyi, deletion of pilS or pilR as well as chpA or pilG in A. baumannii completely abolished transformation, while the absence of pilH did not change the strain’s transformation efficiency


Genomic Context

Location: 3488513..3500081
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H0N27_RS16305 (H0N27_16260) - 3489005..3490306 (+) 1302 WP_000805827.1 homoserine dehydrogenase -
  H0N27_RS16310 (H0N27_16265) thrC 3490362..3491501 (+) 1140 WP_000063593.1 threonine synthase -
  H0N27_RS16315 (H0N27_16270) pbpG 3491608..3492654 (-) 1047 WP_001984577.1 D-alanyl-D-alanine endopeptidase PBP7/8 -
  H0N27_RS16320 (H0N27_16275) - 3492867..3493502 (+) 636 WP_000633799.1 response regulator -
  H0N27_RS16325 (H0N27_16280) pilS 3493513..3495081 (+) 1569 WP_206677389.1 sensor histidine kinase Regulator
  H0N27_RS16330 (H0N27_16285) pilR 3495106..3496527 (+) 1422 WP_168727019.1 sigma-54-dependent transcriptional regulator Regulator
  H0N27_RS16335 (H0N27_16290) - 3496531..3497715 (-) 1185 WP_000939107.1 S41 family peptidase -
  H0N27_RS16340 (H0N27_16295) gpmI 3497731..3499278 (-) 1548 WP_031963978.1 2,3-bisphosphoglycerate-independent phosphoglycerate mutase -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  pilS pilR positive effect
  pilR pilA positive effect
  hns pilA negative effect
  hns pilQ negative effect
  hns pilT negative effect
  hns comA/comEC negative effect
  hns comF negative effect
  hns dprA negative effect
  hns comEA negative effect

Sequence

Protein


Download         Length: 522 a.a.        Molecular weight: 59227.76 Da        Isoelectric Point: 6.4995

>NTDB_id=1044 H0N27_RS16325 WP_206677389.1 3493513..3495081(+) (pilS) [Acinetobacter baumannii strain A118]
MQFPLNSSLSHTIFRLGTWYGLYRLIIAVSLNIILVLTDAQTDNSLQQPALYSYTLLGYSLLSLVQLLCFKFIATQATRQ
LILFFIVDIICLSLLTFSVGEPNLQLSLLYVIAIFTSAILLSARMSLLITLLAVIAVIYQRFVGSLFDYNNLNTIGNSAL
LAFLFFVVHGIGQIAVQRFKLLEALTFHQSIELYQLQNINRYILEQIEEGYLVLDENYDIVVSNPAACSLLGIPPQFANE
KYPLVKWHADLFEILKFGDLKEGDRFIFESRLSAYSINIKVQHLLVPQQTLTLLILQDAQQINQQAQQLKLAALGQLSAS
IAHEIRNPLAAIVQANELLKDSDPEQQNTLRHMIGKQTKRIDSIVQDTLGLARSERTHPIQIDVKHFIDTLLEEDLFDVK
HSIQLKISDSSLKFLFDEKQLRQVMINLVRNALRHNAPDSPYITINIHSQTNKIYIDVIDYGEGVSKRDISQLFKPFFST
EINGTGLGLYLSHSFCEANHAKLTYVEQKQGACFRIECPIIY

Nucleotide


Download         Length: 1569 bp        

>NTDB_id=1044 H0N27_RS16325 WP_206677389.1 3493513..3495081(+) (pilS) [Acinetobacter baumannii strain A118]
ATGCAATTTCCCTTAAATTCATCCTTGTCCCATACGATTTTTCGTTTGGGCACTTGGTATGGCTTATACCGTCTTATTAT
TGCGGTTAGCCTGAATATTATTTTAGTTTTAACCGATGCGCAAACTGATAACAGTTTGCAGCAACCTGCCTTATATTCCT
ACACACTACTCGGGTATTCGCTCTTAAGTCTTGTTCAGCTGTTATGCTTTAAATTTATTGCCACTCAAGCCACGCGACAG
CTTATTTTATTTTTTATTGTTGATATTATTTGTTTGAGTTTACTGACTTTTTCTGTGGGTGAACCAAATTTACAACTCAG
TTTACTCTATGTAATTGCCATCTTTACTTCGGCGATTTTACTCAGCGCAAGAATGTCTTTGCTGATTACATTGCTTGCGG
TTATCGCTGTTATTTACCAACGATTTGTAGGTAGTCTATTTGACTATAACAATCTAAATACTATTGGTAATAGCGCTCTC
TTAGCTTTTTTATTTTTTGTGGTTCACGGTATTGGCCAAATTGCTGTGCAGCGCTTTAAATTACTTGAAGCACTCACCTT
TCACCAGTCAATTGAACTTTATCAACTCCAAAATATTAACCGTTATATCTTAGAACAAATTGAAGAAGGCTATTTAGTTT
TAGATGAGAATTATGACATCGTTGTCAGTAATCCTGCGGCCTGTTCACTCCTGGGTATTCCCCCTCAATTTGCCAATGAA
AAATATCCTTTAGTGAAGTGGCATGCCGATTTATTTGAAATTTTAAAGTTTGGTGACTTAAAAGAAGGTGACCGATTCAT
TTTTGAGTCTCGGCTATCGGCATACTCCATCAACATTAAAGTGCAACACCTTTTGGTCCCACAACAAACGTTAACTCTGC
TCATTTTACAAGATGCCCAACAAATTAATCAGCAAGCCCAGCAGCTTAAGCTTGCTGCTCTAGGACAGCTCTCAGCAAGT
ATTGCACATGAGATCCGCAACCCTCTCGCTGCAATCGTACAAGCAAATGAGTTACTGAAAGATAGCGATCCAGAACAACA
AAATACTTTGCGTCATATGATTGGCAAACAAACCAAACGCATTGATAGTATTGTTCAAGATACTTTAGGGCTTGCTCGTA
GTGAAAGAACACATCCTATCCAGATCGATGTAAAACACTTCATAGATACATTGCTCGAAGAAGATTTATTTGACGTTAAA
CATTCAATTCAACTTAAAATTTCGGATAGTTCTTTAAAATTTTTATTTGATGAAAAACAATTAAGACAAGTCATGATCAA
TCTGGTGAGAAATGCTTTACGGCATAATGCCCCCGACTCACCGTACATTACGATCAACATTCATTCACAGACAAATAAAA
TTTATATTGATGTGATCGATTACGGTGAAGGTGTTTCAAAACGTGATATATCTCAATTATTTAAACCATTTTTTAGTACC
GAAATTAATGGAACTGGTTTAGGATTATATTTGTCTCATAGTTTTTGTGAGGCGAACCATGCAAAGCTCACCTATGTAGA
GCAAAAACAAGGGGCATGCTTCAGAATAGAATGCCCAATAATATATTGA

Domains

Predicted by InterproScan.

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c)

(417-518)

His Kinase A (phospho-acceptor) domain (HisKA)

(313-375)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Nina Vesel et al. (2021) Pilus Production in Acinetobacter baumannii Is Growth Phase Dependent and Essential for Natural Transformation. Journal of Bacteriology 203(8):e00034-21. [PMID: 33495250]