Detailed information    

experimental Experimentally validated

Overview

Name   comF   Type   Machinery gene
Locus tag   H0N27_RS01930 Genome accession   NZ_CP059039
Coordinates   415434..416069 (-) Length   211 a.a.
NCBI ID   WP_000472273.1    Uniprot ID   A0A9P3CZD1
Organism   Acinetobacter baumannii strain A118     
Function   ssDNA transport into the cell   
DNA binding and uptake

Function

Mutant studies with A. baumannii A118 confirmed that pilA, pilQ (secretin, comQ homologue), pilT (retraction ATPase), comEA (DNA-binding protein), comF (pilotin) and dprA (DNA protecting protein) are required for natural transformation


Genomic Context

Location: 410434..421069
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H0N27_RS01895 (H0N27_01895) mlaE 410770..411546 (+) 777 WP_001091949.1 lipid asymmetry maintenance ABC transporter permease subunit MlaE -
  H0N27_RS01900 (H0N27_01900) - 411546..412226 (+) 681 WP_000842362.1 outer membrane lipid asymmetry maintenance protein MlaD -
  H0N27_RS01905 (H0N27_01905) - 412253..412894 (+) 642 WP_001093907.1 phospholipid-binding protein MlaC -
  H0N27_RS01910 (H0N27_01910) - 412906..413193 (+) 288 WP_000250997.1 lipid asymmetry maintenance protein MlaB -
  H0N27_RS01915 (H0N27_01915) - 413263..414276 (-) 1014 WP_000888321.1 CorA family divalent cation transporter -
  H0N27_RS01920 (H0N27_01920) - 414434..415015 (+) 582 WP_031379916.1 TIGR00730 family Rossman fold protein -
  H0N27_RS01925 (H0N27_01925) - 415030..415431 (+) 402 WP_000849705.1 NUDIX hydrolase -
  H0N27_RS01930 (H0N27_01930) comF 415434..416069 (-) 636 WP_000472273.1 ComF family protein Machinery gene
  H0N27_RS01935 (H0N27_01935) recG 416062..418107 (-) 2046 WP_031379915.1 ATP-dependent DNA helicase RecG -
  H0N27_RS01940 (H0N27_01940) - 418128..418943 (+) 816 WP_000547772.1 NAD-dependent epimerase/dehydratase family protein -
  H0N27_RS01945 (H0N27_01945) adeT2 419023..420009 (+) 987 WP_168726710.1 putative multidrug efflux protein AdeT2 -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  hns comF negative effect
  hns pilQ negative effect
  hns pilT negative effect
  hns comA/comEC negative effect
  hns pilA negative effect
  pilR pilA positive effect
  hns dprA negative effect
  hns comEA negative effect
  pilS pilR positive effect

Sequence

Protein


Download         Length: 211 a.a.        Molecular weight: 24565.69 Da        Isoelectric Point: 9.7140

>NTDB_id=1042 H0N27_RS01930 WP_000472273.1 415434..416069(-) (comF) [Acinetobacter baumannii strain A118]
MFKFLNPQYLFQLLSPCLLCEIGTREKYSLCKECWEQLPWLKQTIQRNDQSVLVACHYAYPINRIIQQFKYEQKLHYQTL
LAEVLQQLKFPKVQAIVPMPISKQRLTERGFNQSLLLANLLSKQLKIPVWQPVQRLNEHSQKGLSRLERFENIEQQFVAL
TQEKRRYRRVLIIDDVITTGSSIHALSQALKQLGCTSIHASCLAAASSTSY

Nucleotide


Download         Length: 636 bp        

>NTDB_id=1042 H0N27_RS01930 WP_000472273.1 415434..416069(-) (comF) [Acinetobacter baumannii strain A118]
ATGTTTAAGTTTTTAAACCCACAGTATCTTTTCCAGTTGCTGTCCCCTTGCCTACTTTGCGAAATAGGAACACGGGAAAA
ATATTCTCTTTGTAAAGAATGCTGGGAACAGCTACCGTGGCTTAAGCAAACCATTCAGCGCAATGATCAGTCTGTTCTTG
TGGCCTGTCACTACGCCTATCCAATTAACCGGATTATTCAGCAGTTTAAATATGAACAAAAATTACATTACCAAACCTTA
CTCGCTGAAGTTTTACAGCAATTAAAATTTCCTAAGGTACAAGCGATTGTACCCATGCCTATTTCTAAACAACGTTTAAC
TGAACGTGGTTTTAATCAATCCTTATTACTCGCCAATCTTTTGAGTAAGCAATTAAAAATACCAGTTTGGCAGCCTGTAC
AACGCTTAAATGAGCATTCTCAAAAGGGATTATCTCGACTCGAAAGATTTGAAAATATTGAACAGCAATTTGTTGCGCTC
ACTCAAGAAAAACGGCGTTATCGTCGTGTACTGATTATTGATGATGTAATCACAACAGGAAGTTCAATTCACGCTTTAAG
TCAGGCGCTAAAACAGCTAGGGTGTACATCTATTCATGCCAGTTGCCTAGCTGCTGCTTCATCTACATCATATTAA

Domains

Predicted by InterproScan.

Phosphoribosyl transferase domain (Pribosyltran)

(114-208)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Acinetobacter baumannii D1279779

99.052

100

0.991

Multiple sequence alignment    



References

[1] Nina Vesel et al. (2021) Pilus Production in Acinetobacter baumannii Is Growth Phase Dependent and Essential for Natural Transformation. Journal of Bacteriology 203(8):e00034-21. [PMID: 33495250]
[2] Casin Le et al. (2021) Involvement of the Histone-Like Nucleoid Structuring Protein (H-NS) in Acinetobacter baumannii's Natural Transformation. Pathogens (Basel, Switzerland) 10(9):1083. [PMID: 34578115]