Detailed information    

experimental Experimentally validated

Overview

Name   comF   Type   Machinery gene
Locus tag   ABD1_RS15375 Genome accession   NC_020547
Coordinates   3247882..3248517 (+) Length   211 a.a.
NCBI ID   WP_000472269.1    Uniprot ID   A0A383KXM5
Organism   Acinetobacter baumannii D1279779     
Function   ssDNA transport into the cell   
DNA binding and uptake

Function

We examined the 33 knockout mutants by comparing their transformation efficiency with wild-type W068. No knockout strains were affected in growth, but 28 of these mutants were severely or completely impaired in natural transformability. These mutations were in 18 genes related to T4P (pilF, pilQ, tsaP, pilM, pilN, pilO, pilP, pilB, pilC, pilT, pilD, pilE, pilY2, pilY1, pilX, pilW, pilV, and fimU), six genes related to DNA uptake and processing (comEA, comA, comF, priA, dprA, and recA), two genes related to T2SS (xcpV and xcpW), and the crp and tonB2 genes.


Genomic Context

Location: 3242882..3253517
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABD1_RS15360 (ABD1_29780) adeT2 3243942..3244928 (-) 987 WP_000712907.1 putative multidrug efflux protein AdeT2 -
  ABD1_RS15365 (ABD1_29790) - 3245008..3245823 (-) 816 WP_000547774.1 NAD-dependent epimerase/dehydratase family protein -
  ABD1_RS15370 (ABD1_29800) recG 3245844..3247889 (+) 2046 WP_000204943.1 ATP-dependent DNA helicase RecG -
  ABD1_RS15375 (ABD1_29810) comF 3247882..3248517 (+) 636 WP_000472269.1 ComF family protein Machinery gene
  ABD1_RS15380 (ABD1_29820) - 3248520..3248921 (-) 402 WP_000849705.1 NUDIX hydrolase -
  ABD1_RS15385 (ABD1_29830) - 3248936..3249517 (-) 582 WP_001084310.1 TIGR00730 family Rossman fold protein -
  ABD1_RS15390 (ABD1_29840) - 3249675..3250688 (+) 1014 WP_000888321.1 CorA family divalent cation transporter -
  ABD1_RS15395 (ABD1_29850) - 3250758..3251045 (-) 288 WP_000250997.1 STAS domain-containing protein -
  ABD1_RS15400 (ABD1_29860) - 3251057..3251698 (-) 642 WP_001093907.1 MlaC/ttg2D family ABC transporter substrate-binding protein -
  ABD1_RS15405 (ABD1_29870) - 3251725..3252405 (-) 681 WP_000842362.1 outer membrane lipid asymmetry maintenance protein MlaD -
  ABD1_RS15410 (ABD1_29880) mlaE 3252405..3253181 (-) 777 WP_001091949.1 lipid asymmetry maintenance ABC transporter permease subunit MlaE -

Sequence

Protein


Download         Length: 211 a.a.        Molecular weight: 24521.60 Da        Isoelectric Point: 9.5702

>NTDB_id=1071 ABD1_RS15375 WP_000472269.1 3247882..3248517(+) (comF) [Acinetobacter baumannii D1279779]
MFKFLNPQYLFQLLSPCLLCEIGTREKYSLCKECWEQLPWLKQTIQRNDQSVLVACHYAYPINRIIQQFKYEQKLHYQTL
LAEVLQQLKFPKVQAIVPMPISKQRLTERGFNQSLLLANLLGKQLKIPVWQPVQRLNEHSQKGLSRLERFENIEQQFVAL
TQENRRYRRVLIIDDVITTGSSIHALSQALKQLGCTSIHASCLAAASSTSY

Nucleotide


Download         Length: 636 bp        

>NTDB_id=1071 ABD1_RS15375 WP_000472269.1 3247882..3248517(+) (comF) [Acinetobacter baumannii D1279779]
ATGTTTAAGTTTTTAAACCCACAGTATCTTTTTCAGTTGCTGTCCCCTTGCCTACTTTGCGAAATAGGAACACGGGAAAA
ATACTCTCTTTGTAAAGAATGCTGGGAACAGCTACCGTGGCTTAAGCAAACCATTCAGCGCAATGATCAGTCTGTTCTTG
TGGCCTGTCACTACGCCTATCCAATTAACCGGATCATTCAGCAGTTTAAATATGAACAAAAATTACATTACCAAACCTTA
CTCGCTGAAGTTTTACAGCAATTAAAATTTCCTAAGGTACAAGCGATTGTACCCATGCCTATTTCTAAACAACGTTTAAC
TGAACGTGGTTTTAATCAATCCTTATTACTTGCCAATCTTTTGGGTAAGCAATTAAAAATACCAGTTTGGCAGCCTGTAC
AACGCTTAAATGAGCATTCTCAAAAGGGATTATCTCGACTCGAAAGATTTGAAAATATTGAACAGCAATTTGTTGCGCTC
ACTCAAGAAAACCGGCGTTATCGTCGTGTGCTGATTATTGATGATGTAATCACCACAGGAAGTTCAATTCACGCTTTAAG
TCAGGCGCTAAAACAGCTAGGGTGTACATCTATTCATGCCAGTTGCCTAGCTGCTGCTTCATCTACATCATATTAA

Domains

Predicted by InterproScan.

Phosphoribosyl transferase domain (Pribosyltran)

(160-208)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A383KXM5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Acinetobacter baumannii strain A118

99.052

100

0.991

Multiple sequence alignment    



References

[1] Yuan Hu et al. (2021) Natural Transformation in Acinetobacter baumannii W068: A Genetic Analysis Reveals the Involvements of the CRP, XcpV, XcpW, TsaP, and TonB2. Frontiers in Microbiology 12:738034. [PMID: 35126321]