Detailed information    

experimental Experimentally validated

Overview

Name   dprA   Type   Machinery gene
Locus tag   ACIAD_RS00975 Genome accession   NC_005966
Coordinates   204848..205999 (-) Length   383 a.a.
NCBI ID   WP_004930462.1    Uniprot ID   Q6FFH8
Organism   Acinetobacter baylyi ADP1     
Function   ssDNA binding; loading RecA onto ssDNA   
DNA processing

Function

DprA initiates the integration of the incoming DNA into the chromosome by loading RecA onto ssDNA.


Genomic Context

Location: 199848..210999
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACIAD_RS00955 (ACIAD0205) - 200065..201624 (+) 1560 WP_011181993.1 ABC transporter ATP-binding protein -
  ACIAD_RS00960 (ACIAD0206) - 201664..202770 (+) 1107 WP_004930471.1 ABC transporter permease -
  ACIAD_RS00965 (ACIAD0207) - 202783..203700 (+) 918 WP_004930467.1 ABC transporter permease -
  ACIAD_RS00970 (ACIAD0208) - 204236..204808 (-) 573 WP_004930465.1 L-threonylcarbamoyladenylate synthase -
  ACIAD_RS00975 (ACIAD0209) dprA 204848..205999 (-) 1152 WP_004930462.1 DNA-processing protein DprA Machinery gene
  ACIAD_RS00980 (ACIAD0210) tsaP 206017..207174 (-) 1158 WP_004930460.1 LysM peptidoglycan-binding domain-containing protein Machinery gene
  ACIAD_RS00985 (ACIAD0211) def 207295..207819 (+) 525 WP_004930458.1 peptide deformylase -
  ACIAD_RS00990 (ACIAD0212) - 207904..208335 (+) 432 WP_004930457.1 DUF2946 family protein -
  ACIAD_RS00995 (ACIAD0214) - 208438..210426 (+) 1989 WP_004930454.1 TonB-dependent copper receptor -
  ACIAD_RS17210 - 210507..210638 (+) 132 Protein_191 hypothetical protein -

Sequence

Protein


Download         Length: 383 a.a.        Molecular weight: 42513.12 Da        Isoelectric Point: 6.5346

>NTDB_id=1021 ACIAD_RS00975 WP_004930462.1 204848..205999(-) (dprA) [Acinetobacter baylyi ADP1]
MITILSSHHYHTIKVWYVLQHSLSGFKKLIHYFGDCETALQPQRLSDWQKLGLHANHLKRLEEVHTLQGQQQFEKMLAQL
QTHTDFIVTPDDPAYPSQLIPYQDRPPILFGQGSIQSLIQPQIAVVGSRKPSPHGRQVAYDFSYFLSEKGFYITSGLAHG
IDEAAHQGSLVHHRAIAVVGTGLDLCYPSGNKALKQHIRDQGGAIVSEFLPGTPPLQAHFPRRNRIVSGLSLGVLVVEAT
LKSGSLITANKAAEQGKVVFAIPGHIYSEFHQGCHQLIREGAILVDHPEQIIEDLALPTHWHSQSTASGEANADDSGKTD
DADLPDDLLPLYQLLDWQGQDLDQLAQHYQGSTAELTAQLMELELLGVCIQQSGRYLRCRSPY

Nucleotide


Download         Length: 1152 bp        

>NTDB_id=1021 ACIAD_RS00975 WP_004930462.1 204848..205999(-) (dprA) [Acinetobacter baylyi ADP1]
ATGATTACAATACTTTCATCACATCACTACCACACCATCAAGGTGTGGTATGTATTACAGCATTCACTTTCAGGCTTCAA
AAAACTCATTCATTATTTTGGTGATTGTGAAACAGCACTACAACCCCAGCGTTTAAGTGACTGGCAAAAGCTCGGATTAC
ATGCCAATCATCTAAAGCGCCTAGAGGAAGTCCATACATTACAAGGACAGCAGCAATTCGAAAAGATGCTTGCTCAGCTT
CAAACCCATACCGATTTTATTGTGACACCCGATGATCCCGCTTATCCATCGCAGTTAATTCCCTATCAGGATCGCCCTCC
CATTCTATTTGGTCAAGGCTCAATACAAAGCCTGATCCAACCACAAATTGCAGTCGTGGGTAGTCGTAAACCCAGCCCTC
ATGGTCGACAGGTAGCATATGACTTTTCTTATTTTTTAAGTGAAAAAGGTTTTTATATTACCAGCGGTTTAGCCCATGGA
ATTGATGAAGCTGCACATCAGGGGAGTCTGGTACACCACCGCGCAATAGCTGTGGTCGGAACAGGGTTAGATCTATGCTA
TCCATCAGGCAATAAAGCCTTGAAACAGCACATACGAGATCAGGGTGGTGCGATCGTTAGTGAATTCTTGCCAGGAACAC
CACCTTTACAAGCCCATTTTCCGCGACGCAACCGTATCGTGAGTGGCTTAAGTCTAGGTGTTCTGGTGGTTGAAGCGACA
TTGAAAAGTGGCTCATTAATTACCGCCAATAAAGCGGCAGAACAAGGTAAAGTTGTTTTTGCGATTCCAGGCCATATTTA
TAGTGAGTTTCATCAAGGCTGCCATCAACTGATTCGCGAAGGTGCGATTTTAGTCGATCACCCCGAACAGATCATTGAAG
ACCTGGCACTTCCGACCCATTGGCATAGTCAGTCTACTGCATCGGGAGAAGCCAATGCCGATGATTCAGGCAAAACCGAT
GATGCTGATTTACCTGATGACTTGCTTCCCCTCTATCAGCTTCTAGACTGGCAAGGTCAAGATCTGGATCAGTTGGCACA
ACACTATCAGGGTTCAACAGCCGAACTAACAGCTCAATTGATGGAACTTGAACTACTGGGTGTGTGTATTCAACAATCAG
GTCGCTATCTGCGTTGTAGAAGCCCCTATTAA

Domains

Predicted by InterproScan.

DNA recombination-mediator protein A (DNA_processg_A)

(86-286)

DprA winged helix domain (DprA_WH)

(321-374)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q6FFH8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Acinetobacter baumannii D1279779

70.079

100

0.71
  dprA Acinetobacter baumannii strain A118

70.079

100

0.71
  dprA Vibrio cholerae strain A1552

41.742

89.757

0.375

Multiple sequence alignment    



References

[1] Mikkel M Liljegren et al. (2024) The recombination initiation functions DprA and RecFOR suppress microindel mutations in Acinetobacter baylyi ADP1. Molecular Microbiology 122(1):1-10. [PMID: 38760330]
[2] Beate Averhoff et al. (2021) Natural transformation in Gram-negative bacteria thriving in extreme environments: from genes and genomes to proteins, structures and regulation. Extremophiles : Life Under Extreme Conditions 25(5-6):425-436. [PMID: 34542714]
[3] Nils Hülter et al. (2017) Costs and benefits of natural transformation in Acinetobacter baylyi. BMC Microbiology 17(1):34. [PMID: 28202049]