Detailed information    

experimental Experimentally validated

Overview

Name   dprA   Type   Machinery gene
Locus tag   A1552VC_RS00200 Genome accession   NZ_CP028894
Coordinates   38664..39779 (+) Length   371 a.a.
NCBI ID   WP_000654772.1    Uniprot ID   A0A0H5U567
Organism   Vibrio cholerae strain A1552     
Function   ssDNA binding; loading RecA onto ssDNA   
DNA processing

Function

Once inside the cytoplasm, Ssb and DprA bind the ssDNA and facilitate loading of RecA, which ultimately catalyzes recombination with the bacterial chromosome.


Genomic Context

Location: 33664..44779
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A1552VC_RS00180 (A1552VC_00034) rsmB 34587..35867 (-) 1281 WP_001102807.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  A1552VC_RS00185 (A1552VC_00035) fmt 35923..36870 (-) 948 WP_000083540.1 methionyl-tRNA formyltransferase -
  A1552VC_RS00190 (A1552VC_00036) def 36888..37397 (-) 510 WP_000115010.1 peptide deformylase -
  A1552VC_RS00195 (A1552VC_00037) - 37537..38664 (+) 1128 WP_000113383.1 LysM peptidoglycan-binding domain-containing protein -
  A1552VC_RS00200 (A1552VC_00038) dprA 38664..39779 (+) 1116 WP_000654772.1 DNA-processing protein DprA Machinery gene
  A1552VC_RS00205 (A1552VC_00039) - 39782..40258 (+) 477 WP_000979769.1 DUF494 family protein -
  A1552VC_RS00210 (A1552VC_00040) - 40274..40840 (+) 567 WP_000087525.1 type I DNA topoisomerase -
  A1552VC_RS00215 (A1552VC_00041) - 40913..42046 (-) 1134 WP_001265400.1 5-(carboxyamino)imidazole ribonucleotide synthase -
  A1552VC_RS00220 (A1552VC_00042) purE 42050..42535 (-) 486 WP_000215768.1 5-(carboxyamino)imidazole ribonucleotide mutase -
  A1552VC_RS00225 (A1552VC_00043) - 42702..43259 (+) 558 WP_001911770.1 L-threonylcarbamoyladenylate synthase -
  A1552VC_RS00230 (A1552VC_00044) hemF 43278..44195 (+) 918 WP_000443976.1 oxygen-dependent coproporphyrinogen oxidase -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  tfoX dprA positive effect
  tfoX pilO positive effect
  tfoX pilB positive effect
  tfoX pilP positive effect
  tfoX pilA positive effect
  tfoX pilD positive effect
  tfoX pilM positive effect
  tfoX pilN positive effect
  tfoX comEA positive effect
  hapR comEA positive effect
  crp comEA positive effect
  qstR comEA positive effect
  tfoX pilQ positive effect
  tfoX qstR positive effect
  qstR comEC positive effect
  qstR comF positive effect
  qstR comM positive effect
  crp qstR positive effect
  hapR qstR positive effect
  tfoX comEC positive effect
  hapR comEC positive effect
  tfoX pilC positive effect
  chiS tfoX positive effect
  hapR dns negative effect

Sequence

Protein


Download         Length: 371 a.a.        Molecular weight: 40035.01 Da        Isoelectric Point: 7.1834

>NTDB_id=1152 A1552VC_RS00200 WP_000654772.1 38664..39779(+) (dprA) [Vibrio cholerae strain A1552]
MKDQDLAAWLALCFTPKLGSKTISHLLATRLPAQLQSFTPKQWLACGLKPEQLVFLTTQAAKQAEQCLQWRSAANNRYIV
TPHCPLYPRLLKEINSSPPVLFIEGIWEAVHDPAVAIVGSRNASVDGRQIARQFATELAQSGLVVTSGLALGIDGYAHDG
ALQAQGQTVAVLGSGLAQVYPKQHQGLAERIIAQGALVSEFAPHTPPKADHFPRRNRIISGLSLGVVVVEAAEKSGSLIT
ARYAAEQGREVFVVPGSIFNAASQGSNQLIRQGACLVQSVQQIHQELKNALTWSLSEQVPYQATLFSAVQSDEELPFPEL
LANVGIEATPIDILASRTQIPVQDIMMQLLELELLGHVVAVPGGYIRKGRG

Nucleotide


Download         Length: 1116 bp        

>NTDB_id=1152 A1552VC_RS00200 WP_000654772.1 38664..39779(+) (dprA) [Vibrio cholerae strain A1552]
ATGAAAGATCAGGATTTAGCGGCATGGTTGGCGCTCTGTTTTACTCCTAAACTAGGCAGCAAAACCATTTCTCACCTGCT
TGCGACCCGTTTGCCAGCCCAGTTGCAAAGTTTTACGCCTAAGCAATGGTTGGCCTGCGGGCTTAAGCCCGAACAACTGG
TGTTTTTAACCACTCAAGCGGCTAAACAAGCCGAGCAGTGTTTGCAATGGCGATCAGCAGCCAATAACCGCTATATCGTC
ACTCCTCATTGCCCGCTTTACCCTCGTTTATTGAAAGAGATTAACTCATCGCCTCCCGTCCTGTTTATTGAAGGAATATG
GGAAGCGGTGCATGACCCTGCGGTGGCTATCGTCGGTAGCCGCAATGCCAGTGTTGATGGGCGGCAGATCGCTCGCCAGT
TTGCCACTGAGCTCGCGCAGTCAGGTTTAGTGGTCACCAGTGGTTTAGCGCTTGGTATTGATGGCTATGCGCACGATGGC
GCTTTGCAAGCACAAGGGCAAACCGTAGCAGTATTAGGTTCAGGGCTGGCGCAGGTTTACCCCAAACAGCATCAAGGGTT
AGCGGAGCGAATCATCGCCCAAGGGGCCTTGGTTTCTGAGTTTGCCCCTCACACACCGCCTAAAGCCGATCACTTTCCGC
GCCGTAACCGAATTATCAGCGGCTTATCGCTCGGTGTTGTGGTGGTAGAAGCTGCGGAGAAAAGCGGCTCACTCATCACT
GCACGCTACGCGGCTGAGCAAGGGCGTGAGGTCTTTGTGGTTCCCGGATCAATTTTTAATGCCGCCAGCCAAGGTAGCAA
CCAATTGATTCGCCAAGGCGCTTGTTTGGTGCAAAGTGTGCAACAAATTCATCAAGAGCTCAAAAATGCGCTGACTTGGT
CACTCTCTGAACAAGTTCCTTATCAAGCAACACTTTTTTCTGCTGTACAGAGCGATGAAGAATTGCCATTTCCCGAGCTG
TTAGCTAACGTAGGAATAGAAGCTACACCTATTGATATTCTCGCAAGCCGGACCCAGATACCGGTGCAAGATATCATGAT
GCAGCTCTTGGAGCTTGAGCTCCTTGGGCATGTGGTTGCAGTACCTGGTGGCTATATTAGAAAGGGGAGAGGCTAG

Domains

Predicted by InterproScan.

DprA winged helix domain (DprA_WH)

(319-364)

DNA recombination-mediator protein A (DNA_processg_A)

(77-278)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H5U567

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Vibrio campbellii strain DS40M4

58.108

100

0.583
  dprA Glaesserella parasuis strain SC1401

43.883

100

0.445
  dprA Legionella pneumophila strain ERS1305867

42.895

100

0.443
  dprA Haemophilus influenzae Rd KW20

43.288

97.855

0.424
  dprA Neisseria meningitidis strain C311

37.845

100

0.407
  dprA Neisseria meningitidis MC58

37.845

100

0.407
  dprA Thermus thermophilus HB27

37.255

100

0.398
  dprA Neisseria gonorrhoeae strain FA1090

36.658

100

0.396
  dprA Acinetobacter baumannii D1279779

43.505

89.218

0.388
  dprA Neisseria gonorrhoeae MS11

35.84

100

0.385
  dprA Acinetobacter baumannii strain A118

43.202

89.218

0.385
  dprA Acinetobacter baylyi ADP1

41.742

89.757

0.375

Multiple sequence alignment    



References

[1] Triana N Dalia et al. (2025) DprA recruits ComM to facilitate recombination during natural transformation in Gram-negative bacteria. Proceedings of The National Academy of Sciences of The United States of America 122(15):e2421764122. [PMID: 40215278]
[2] Patrick Seitz et al. (2013) DNA-uptake machinery of naturally competent Vibrio cholerae. Proceedings of The National Academy of Sciences of The United States of America 110(44):17987-92. [PMID: 24127573]
[3] Mirella Lo Scrudato et al. (2012) The regulatory network of natural competence and transformation of Vibrio cholerae. PLoS Genetics 8(6):e1002778. [PMID: 22737089]
[4] Gaia Suckow et al. (2011) Quorum sensing contributes to natural transformation of Vibrio cholerae in a species-specific manner. Journal of Bacteriology 193(18):4914-24. [PMID: 21784943]