Detailed information    

experimental Experimentally validated

Overview

Name   dprA   Type   Machinery gene
Locus tag   NMB_RS00600 Genome accession   NC_003112
Coordinates   124922..126115 (+) Length   397 a.a.
NCBI ID   WP_002224767.1    Uniprot ID   Q9K1K1
Organism   Neisseria meningitidis MC58     
Function   ssDNA binding; loading RecA onto ssDNA   
DNA processing

Function

In the dprA null background, the transformation rates were almost not detectable.


Genomic Context

Location: 119922..131115
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMB_RS00585 (NMB0113) - 120845..121441 (+) 597 WP_002224764.1 DUF4390 domain-containing protein -
  NMB_RS00590 (NMB0114) - 121441..123561 (+) 2121 WP_002224765.1 sensor histidine kinase -
  NMB_RS00595 (NMB0115) - 123554..124831 (+) 1278 WP_002224766.1 sigma-54-dependent transcriptional regulator -
  NMB_RS00600 (NMB0116) dprA 124922..126115 (+) 1194 WP_002224767.1 DNA-processing protein DprA Machinery gene
  NMB_RS00605 (NMB0117) - 126141..126602 (+) 462 WP_002215351.1 DUF494 domain-containing protein -
  NMB_RS00610 (NMB0118) topA 126674..128980 (+) 2307 WP_002224768.1 type I DNA topoisomerase -
  NMB_RS00615 (NMB0119) - 129053..129748 (-) 696 WP_002216227.1 hypothetical protein -
  NMB_RS00620 (NMB0120) - 129768..130319 (-) 552 WP_002215355.1 hypothetical protein -
  NMB_RS00625 (NMB0121) - 130438..130815 (+) 378 WP_002233408.1 helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 397 a.a.        Molecular weight: 42650.06 Da        Isoelectric Point: 6.6117

>NTDB_id=1089 NMB_RS00600 WP_002224767.1 124922..126115(+) (dprA) [Neisseria meningitidis MC58]
MTEDERFAWLQLAFTPYIGAESFLLLMRRFGSAQNALSAPAEQVAALIRHKQALEAWRNAEKRALARQAAEAALEWEMRD
GCRLMLLQDEDFPEMLTQGLTAPPVLFLRGNVQLLHKPSAAIVGSRHATPQAMRIAKDFGKSLGGKGIPVVSGMASGIDT
AAHQGALQAEGGTIAVWGTGIDRIYPPVNKNLAYEIAEKGLIVSEFPIGTRPYAGNFPRRNRLIAALSQVTLVVEAALES
GSLITARLAAEMGREVMAVPGSIDNPHSKGCHKLIKDGAKLVECLDDILNECPGLLQNTGASSYSINKGIPEKRITAVQT
ASDQLSLPEGKMPSEKTENRPVGGSILDRMGFDPVHPDVLAGQLAMPAADLYAALLELELDGSVAAMPGGRYQRIRT

Nucleotide


Download         Length: 1194 bp        

>NTDB_id=1089 NMB_RS00600 WP_002224767.1 124922..126115(+) (dprA) [Neisseria meningitidis MC58]
ATGACAGAGGACGAACGTTTCGCGTGGCTGCAATTGGCGTTTACGCCCTATATCGGCGCGGAAAGTTTCCTGCTGCTGAT
GCGCCGTTTCGGCAGCGCGCAAAATGCCCTGTCCGCACCGGCGGAACAGGTGGCGGCACTGATACGGCACAAACAGGCGC
TTGAGGCTTGGCGCAATGCGGAAAAACGCGCTCTGGCGCGGCAGGCGGCAGAAGCGGCATTGGAATGGGAAATGCGGGAC
GGATGCCGCCTGATGCTGCTTCAGGATGAAGATTTTCCCGAAATGCTGACGCAGGGGCTGACCGCGCCACCGGTTTTGTT
TTTGCGCGGCAACGTGCAACTGCTGCACAAACCTTCCGCCGCCATCGTCGGCAGCCGTCATGCCACGCCGCAGGCGATGC
GGATTGCCAAAGATTTCGGCAAGTCGTTGGGTGGGAAAGGCATTCCCGTTGTGTCGGGTATGGCTTCGGGCATCGATACC
GCCGCCCATCAGGGTGCGTTGCAGGCAGAAGGCGGCACCATCGCCGTGTGGGGGACGGGCATAGACCGCATTTATCCGCC
GGTCAACAAAAACCTTGCCTATGAAATCGCCGAAAAAGGATTGATTGTCAGCGAGTTCCCCATCGGCACGCGGCCGTATG
CCGGCAATTTTCCGCGCCGCAACCGCCTGATTGCCGCCCTGTCGCAAGTAACGCTGGTGGTTGAAGCCGCGTTGGAATCC
GGTTCGCTGATTACTGCCAGATTGGCGGCGGAGATGGGGCGCGAAGTGATGGCGGTACCCGGCTCGATAGACAATCCACA
CAGTAAAGGCTGCCACAAACTGATTAAAGACGGCGCAAAATTGGTGGAATGCCTGGACGACATCCTGAACGAATGCCCGG
GGCTATTGCAAAATACGGGTGCTTCATCATATTCTATAAATAAGGGAATACCTGAAAAGCGCATCACTGCCGTTCAGACG
GCATCCGACCAGCTGTCTCTGCCTGAAGGCAAAATGCCGTCTGAAAAGACGGAGAACCGACCCGTCGGCGGCAGTATCTT
GGACAGGATGGGTTTCGACCCAGTTCATCCCGACGTGCTTGCCGGACAGTTGGCTATGCCTGCCGCAGATTTGTATGCCG
CACTGTTGGAATTGGAATTGGACGGCAGCGTTGCCGCAATGCCCGGCGGCAGATACCAGCGTATCCGAACTTGA

Domains

Predicted by InterproScan.

DNA recombination-mediator protein A (DNA_processg_A)

(79-283)

DprA winged helix domain (DprA_WH)

(344-390)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9K1K1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Neisseria meningitidis strain C311

100

100

1
  dprA Neisseria gonorrhoeae strain FA1090

90.176

100

0.906
  dprA Neisseria gonorrhoeae MS11

89.924

100

0.904
  dprA Vibrio cholerae strain A1552

37.845

100

0.407
  dprA Vibrio campbellii strain DS40M4

37.084

100

0.393

Multiple sequence alignment    



References

[1] Eirik Hovland et al. (2017) DprA from Neisseria meningitidis: properties and role in natural competence for transformation. Microbiology (Reading, England) 163(7):1016-1029. [PMID: 28696187]