Detailed information    

experimental Experimentally validated

Overview

Name   dprA   Type   Machinery gene
Locus tag   GTF74_RS00175 Genome accession   NZ_CP047303
Coordinates   38663..39778 (+) Length   371 a.a.
NCBI ID   WP_000654772.1    Uniprot ID   Q9KVU1
Organism   Vibrio cholerae O1 biovar El Tor strain E7946     
Function   ssDNA binding; loading RecA onto ssDNA   
DNA processing

Function

Using Helicobacter pylori and Vibrio cholerae as model systems, it was demonstrated that ComM directly interacts with the DprA winged-helix domain, and that this interaction is critical for DprA to recruit ComM to the recombination site to promote branch migration during NT.


Genomic Context

Location: 33663..44778
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GTF74_RS00155 (GTF74_00155) rsmB 34586..35866 (-) 1281 WP_001102808.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  GTF74_RS00160 (GTF74_00160) fmt 35922..36869 (-) 948 WP_000083540.1 methionyl-tRNA formyltransferase -
  GTF74_RS00165 (GTF74_00165) def 36887..37396 (-) 510 WP_000115010.1 peptide deformylase -
  GTF74_RS00170 (GTF74_00170) - 37536..38663 (+) 1128 WP_000113383.1 LysM peptidoglycan-binding domain-containing protein -
  GTF74_RS00175 (GTF74_00175) dprA 38663..39778 (+) 1116 WP_000654772.1 DNA-processing protein DprA Machinery gene
  GTF74_RS00180 (GTF74_00180) - 39781..40257 (+) 477 WP_000979769.1 DUF494 family protein -
  GTF74_RS00185 (GTF74_00185) - 40273..40839 (+) 567 WP_000087525.1 type I DNA topoisomerase -
  GTF74_RS00190 (GTF74_00190) - 40912..42045 (-) 1134 WP_001265400.1 5-(carboxyamino)imidazole ribonucleotide synthase -
  GTF74_RS00195 (GTF74_00195) purE 42049..42534 (-) 486 WP_000215768.1 5-(carboxyamino)imidazole ribonucleotide mutase -
  GTF74_RS00200 (GTF74_00200) - 42701..43258 (+) 558 WP_001911770.1 L-threonylcarbamoyladenylate synthase -
  GTF74_RS00205 (GTF74_00205) hemF 43277..44194 (+) 918 WP_000443976.1 oxygen-dependent coproporphyrinogen oxidase -

Sequence

Protein


Download         Length: 371 a.a.        Molecular weight: 40035.01 Da        Isoelectric Point: 7.1834

>NTDB_id=1477 GTF74_RS00175 WP_000654772.1 38663..39778(+) (dprA) [Vibrio cholerae O1 biovar El Tor strain E7946]
MKDQDLAAWLALCFTPKLGSKTISHLLATRLPAQLQSFTPKQWLACGLKPEQLVFLTTQAAKQAEQCLQWRSAANNRYIV
TPHCPLYPRLLKEINSSPPVLFIEGIWEAVHDPAVAIVGSRNASVDGRQIARQFATELAQSGLVVTSGLALGIDGYAHDG
ALQAQGQTVAVLGSGLAQVYPKQHQGLAERIIAQGALVSEFAPHTPPKADHFPRRNRIISGLSLGVVVVEAAEKSGSLIT
ARYAAEQGREVFVVPGSIFNAASQGSNQLIRQGACLVQSVQQIHQELKNALTWSLSEQVPYQATLFSAVQSDEELPFPEL
LANVGIEATPIDILASRTQIPVQDIMMQLLELELLGHVVAVPGGYIRKGRG

Nucleotide


Download         Length: 1116 bp        

>NTDB_id=1477 GTF74_RS00175 WP_000654772.1 38663..39778(+) (dprA) [Vibrio cholerae O1 biovar El Tor strain E7946]
ATGAAAGATCAGGATTTAGCGGCATGGTTGGCGCTCTGTTTTACTCCTAAACTAGGCAGCAAAACCATTTCTCACCTGCT
TGCGACCCGTTTGCCAGCCCAGTTGCAAAGTTTTACGCCTAAGCAATGGTTGGCCTGCGGGCTTAAGCCCGAACAACTGG
TGTTTTTAACCACTCAAGCGGCTAAACAAGCCGAGCAGTGTTTGCAATGGCGATCAGCAGCCAATAACCGCTATATCGTC
ACTCCTCATTGCCCGCTTTACCCTCGTTTATTGAAAGAGATTAACTCATCGCCTCCCGTCCTGTTTATTGAAGGAATATG
GGAAGCGGTGCATGACCCTGCGGTGGCTATCGTCGGTAGCCGCAATGCCAGTGTTGATGGGCGGCAGATCGCTCGCCAGT
TTGCCACTGAGCTCGCGCAGTCAGGTTTAGTGGTCACCAGTGGTTTAGCGCTTGGTATTGATGGCTATGCGCACGATGGC
GCTTTGCAAGCACAAGGGCAAACCGTAGCAGTATTAGGTTCAGGGCTGGCGCAGGTTTACCCCAAACAGCATCAAGGGTT
AGCGGAGCGAATCATCGCCCAAGGGGCCTTGGTTTCTGAGTTTGCCCCTCACACACCGCCTAAAGCCGATCACTTTCCGC
GCCGTAACCGAATTATCAGCGGCTTATCGCTCGGTGTTGTGGTGGTAGAAGCTGCGGAGAAAAGCGGCTCACTCATCACT
GCACGCTACGCGGCTGAGCAAGGGCGTGAGGTCTTTGTGGTTCCCGGATCAATTTTTAATGCCGCCAGCCAAGGTAGCAA
CCAATTGATTCGCCAAGGCGCTTGTTTGGTGCAAAGTGTGCAACAAATTCATCAAGAGCTCAAAAATGCGCTGACTTGGT
CACTCTCTGAACAAGTTCCTTATCAAGCAACACTTTTTTCTGCTGTACAGAGCGATGAAGAATTGCCATTTCCCGAGCTG
TTAGCTAACGTAGGAATAGAAGCTACACCTATTGATATTCTCGCAAGCCGGACCCAGATACCGGTGCAAGATATCATGAT
GCAGCTCTTGGAGCTTGAGCTCCTTGGGCATGTGGTTGCAGTACCTGGTGGCTATATTAGAAAGGGGAGAGGCTAG

Domains

Predicted by InterProScan.

DprA winged helix domain (WHD_DprA)

(319-364)

DNA recombination-mediator protein A (DNA_processg_A)

(77-278)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9KVU1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Vibrio cholerae strain A1552

100

100

1
  dprA Vibrio campbellii strain DS40M4

58.108

100

0.583
  dprA Glaesserella parasuis strain SC1401

43.883

100

0.445
  dprA Legionella pneumophila strain ERS1305867

42.895

100

0.443
  dprA Haemophilus influenzae Rd KW20

43.288

97.855

0.424
  dprA Neisseria meningitidis strain C311

37.845

100

0.407
  dprA Neisseria meningitidis MC58

37.845

100

0.407
  dprA Thermus thermophilus HB27

37.255

100

0.398
  dprA Neisseria gonorrhoeae strain FA1090

36.658

100

0.396
  dprA Acinetobacter baumannii D1279779

43.505

89.218

0.388
  dprA Neisseria gonorrhoeae MS11

35.84

100

0.385
  dprA Acinetobacter baumannii strain A118

43.202

89.218

0.385
  dprA Acinetobacter baylyi ADP1

41.742

89.757

0.375

References

[1] Triana N Dalia et al. (2025) DprA recruits ComM to facilitate recombination during natural transformation in Gram-negative bacteria. Proceedings of The National Academy of Sciences of The United States of America 122(15):e2421764122. [PMID: 40215278]