Detailed information    

experimental Experimentally validated

Overview

Name   dprA   Type   Machinery gene
Locus tag   NGFG_RS10355 Genome accession   NC_022240
Coordinates   1906441..1907628 (-) Length   395 a.a.
NCBI ID   WP_020997408.1    Uniprot ID   -
Organism   Neisseria gonorrhoeae MS11     
Function   ssDNA binding; loading RecA onto ssDNA   
DNA processing

Function

Inactivation of dprA completely abrogated transformation of gyrB1-encoding DNA. Loss of transformation in the dprA null mutants was independent of the DNA uptake sequence. DprA mutants exhibited increased RecA-dependent pilin variation suggesting that DprA affects pilin variation.


Genomic Context

Location: 1901441..1912628
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NGFG_RS15315 - 1901492..1901686 (-) 195 WP_167549141.1 hypothetical protein -
  NGFG_RS10330 (NGFG_02467) - 1901700..1902377 (-) 678 WP_020997406.1 opacity family porin -
  NGFG_RS10335 - 1902404..1902628 (+) 225 WP_134814576.1 hypothetical protein -
  NGFG_RS13790 - 1902693..1903489 (-) 797 Protein_1944 IS1595 family transposase -
  NGFG_RS10345 (NGFG_01779) topA 1903576..1905882 (-) 2307 WP_003696216.1 type I DNA topoisomerase -
  NGFG_RS10350 (NGFG_01780) - 1905954..1906415 (-) 462 WP_003690120.1 DUF494 domain-containing protein -
  NGFG_RS10355 (NGFG_01781) dprA 1906441..1907628 (-) 1188 WP_020997408.1 DNA-processing protein DprA Machinery gene
  NGFG_RS10360 (NGFG_01782) - 1907719..1908996 (-) 1278 WP_003690124.1 sigma-54-dependent transcriptional regulator -
  NGFG_RS10365 (NGFG_01783) - 1908989..1911109 (-) 2121 WP_003690126.1 HAMP domain-containing histidine kinase -
  NGFG_RS10370 (NGFG_01784) - 1911109..1911708 (-) 600 WP_003690128.1 DUF4390 domain-containing protein -

Sequence

Protein


Download         Length: 395 a.a.        Molecular weight: 41911.00 Da        Isoelectric Point: 6.3096

>NTDB_id=1120 NGFG_RS10355 WP_020997408.1 1906441..1907628(-) (dprA) [Neisseria gonorrhoeae MS11]
MTEDERFAWLQLAFTPYIGAESFLLLMRSFGSAQNALSAPAEQVAPAVRHKHALEAWRNAEKRALARQAAEAALEWEMRD
GCRLMLLQDEDFPEMLTQGLTAPPVLFLRGNVRLLHKPSAAIVGSRHATPQAMRIAKDFGRALGGKGIPTVSGMASGIDT
AAHQGALEAEGGTIAVWGTGIDRIYPPANKNLAYEIAEKGLIVSEFPIGTRPYAGNFPRRNRLIAALSQVTLVVEAALES
GSLITAGLAAEMGREVMAVPGSIDNPHSKGCHKLIKDGAKLTECLDDILNECPGLLQNTGASSYSINKDTPDTGRRTVQT
AYAPPPAAKMPSEGAAGGTAVGGILDKMGFDPIHPDVLAGQLAMPAADLYAALLELELDGSVAAMPGGRYQRIRT

Nucleotide


Download         Length: 1188 bp        

>NTDB_id=1120 NGFG_RS10355 WP_020997408.1 1906441..1907628(-) (dprA) [Neisseria gonorrhoeae MS11]
ATGACAGAGGACGAACGTTTCGCGTGGCTGCAATTGGCGTTTACGCCCTATATCGGCGCGGAAAGTTTCCTGCTGCTGAT
GCGCAGTTTCGGCAGCGCGCAAAATGCCTTGTCCGCACCGGCGGAACAGGTTGCCCCGGCGGTGCGGCATAAGCACGCGC
TTGAGGCTTGGCGCAATGCGGAAAAACGCGCTTTGGCGCGGCAGGCGGCAGAAGCGGCGTTGGAATGGGAAATGCGGGAC
GGATGCCGTCTGATGCTGCTTCAGGATGAAGATTTCCCCGAAATGCTGACGCAGGGGCTGACCGCGCCGCCGGTTTTGTT
TTTGCGCGGCAACGTGCGGCTGCTGCACAAGCCTTCCGCCGCCATCGTCGGCAGCCGCCATGCCACGCCGCAGGCGATGC
GGATTGCCAAAGATTTCGGCAGGGCGTTGGGTGGGAAAGGCATTCCTACCGTGTCGGGTATGGCTTCGGGCATCGACACC
GCCGCCCATCAGGGCGCACTGGAGGCAGAAGGCGGCACCATCGCCGTGTGGGGGACGGGCATAGACCGCATTTATCCGCC
GGCCAACAAAAACCTTGCCTATGAAATCGCCGAAAAAGGGCTGATTGTCAGCGAGTTCCCCATCGGCACGCGGCCGTATG
CCGGCAACTTTCCGCGCCGCAACCGCCTGATTGCCGCGCTGTCGCAGGTTACGCTGGTGGTCGAGGCCGCCTTGGAATCC
GGTTCGCTGATTACCGCCGGCCTGGCGGCAGAAATGGGGCGCGAAGTGATGGCGGTACCCGGTTCGATAGACAACCCCCA
CAGCAAAGGCTGCCACAAACTGATTAAAGACGGAGCGAAACTGACGGAATGTTTGGACGACATCCTGAACGAATGCCCGG
GGCTATTGCAAAATACGGGTGCTTCATCATATTCTATAAATAAGGATACGCCCGACACGGGCCGCCGCACCGTTCAGACG
GCATACGCCCCGCCGCCTGCCGCAAAAATGCCGTCTGAAGGGGCGGCAGGCGGCACAGCCGTCGGCGGCATACTGGATAA
AATGGGTTTCGACCCCATCCATCCCGACGTGCTTGCCGGACAGTTGGCTATGCCTGCCGCAGATTTGTATGCCGCACTGT
TGGAATTGGAATTGGACGGCAGCGTTGCCGCAATGCCCGGCGGCAGATACCAGCGTATCCGAACTTGA

Domains

Predicted by InterproScan.

DNA recombination-mediator protein A (DNA_processg_A)

(79-282)

DprA winged helix domain (DprA_WH)

(341-388)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Neisseria gonorrhoeae strain FA1090

99.241

100

0.992
  dprA Neisseria meningitidis strain C311

89.924

100

0.904
  dprA Neisseria meningitidis MC58

89.924

100

0.904
  dprA Vibrio cholerae strain A1552

35.84

100

0.385
  dprA Vibrio campbellii strain DS40M4

36.176

100

0.379
  dprA Glaesserella parasuis strain SC1401

42.994

83.733

0.36

Multiple sequence alignment    



References

[1] Paul M Duffin et al. (2016) DprA is required for natural transformation and affects pilin variation in Neisseria gonorrhoeae. Microbiology (Reading, England) 162(9):1620-1628. [PMID: 27469477]