Detailed information    

experimental Experimentally validated

Overview

Name   dprA   Type   Machinery gene
Locus tag   KZH42_RS01320 Genome accession   NZ_CP079941
Coordinates   258351..259544 (+) Length   397 a.a.
NCBI ID   WP_002224767.1    Uniprot ID   Q9K1K1
Organism   Neisseria meningitidis strain C311     
Function   ssDNA binding; loading RecA onto ssDNA   
DNA processing

Function

dprA mutant has a substantially reduced transformation frequency.


Genomic Context

Location: 253351..264544
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KZH42_RS01305 (KZH42_01305) - 254274..254870 (+) 597 WP_002224764.1 DUF4390 domain-containing protein -
  KZH42_RS01310 (KZH42_01310) - 254870..256990 (+) 2121 WP_002224765.1 PAS domain-containing sensor histidine kinase -
  KZH42_RS01315 (KZH42_01315) - 256983..258260 (+) 1278 WP_002224766.1 sigma-54 dependent transcriptional regulator -
  KZH42_RS01320 (KZH42_01320) dprA 258351..259544 (+) 1194 WP_002224767.1 DNA-processing protein DprA Machinery gene
  KZH42_RS01325 (KZH42_01325) - 259570..260031 (+) 462 WP_002215351.1 DUF494 domain-containing protein -
  KZH42_RS01330 (KZH42_01330) topA 260103..262409 (+) 2307 WP_002224768.1 type I DNA topoisomerase -
  KZH42_RS01335 (KZH42_01335) - 262482..263177 (-) 696 WP_002216227.1 hypothetical protein -
  KZH42_RS01340 (KZH42_01340) - 263197..263748 (-) 552 WP_002215355.1 hypothetical protein -
  KZH42_RS01345 (KZH42_01345) - 263927..264244 (+) 318 WP_002216230.1 helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 397 a.a.        Molecular weight: 42650.06 Da        Isoelectric Point: 6.6117

>NTDB_id=1094 KZH42_RS01320 WP_002224767.1 258351..259544(+) (dprA) [Neisseria meningitidis strain C311]
MTEDERFAWLQLAFTPYIGAESFLLLMRRFGSAQNALSAPAEQVAALIRHKQALEAWRNAEKRALARQAAEAALEWEMRD
GCRLMLLQDEDFPEMLTQGLTAPPVLFLRGNVQLLHKPSAAIVGSRHATPQAMRIAKDFGKSLGGKGIPVVSGMASGIDT
AAHQGALQAEGGTIAVWGTGIDRIYPPVNKNLAYEIAEKGLIVSEFPIGTRPYAGNFPRRNRLIAALSQVTLVVEAALES
GSLITARLAAEMGREVMAVPGSIDNPHSKGCHKLIKDGAKLVECLDDILNECPGLLQNTGASSYSINKGIPEKRITAVQT
ASDQLSLPEGKMPSEKTENRPVGGSILDRMGFDPVHPDVLAGQLAMPAADLYAALLELELDGSVAAMPGGRYQRIRT

Nucleotide


Download         Length: 1194 bp        

>NTDB_id=1094 KZH42_RS01320 WP_002224767.1 258351..259544(+) (dprA) [Neisseria meningitidis strain C311]
ATGACAGAGGACGAACGTTTCGCGTGGCTGCAATTGGCGTTTACGCCCTATATCGGCGCGGAAAGTTTCCTGCTGCTGAT
GCGCCGTTTCGGCAGCGCGCAAAATGCCCTGTCCGCACCGGCGGAACAGGTGGCGGCACTGATACGGCACAAACAGGCGC
TTGAGGCTTGGCGCAATGCGGAAAAACGCGCTCTGGCGCGGCAGGCGGCAGAAGCGGCATTGGAATGGGAAATGCGGGAC
GGATGCCGCCTGATGCTGCTTCAGGATGAAGATTTTCCCGAAATGCTGACGCAGGGGCTGACCGCGCCACCGGTTTTGTT
TTTGCGCGGCAACGTGCAACTGCTGCACAAACCTTCCGCCGCCATCGTCGGCAGCCGTCATGCCACGCCGCAGGCGATGC
GGATTGCCAAAGATTTCGGCAAGTCGTTGGGTGGGAAAGGCATTCCCGTTGTGTCGGGTATGGCTTCGGGCATCGATACC
GCCGCCCATCAGGGTGCGTTGCAGGCAGAAGGCGGCACCATCGCCGTGTGGGGGACGGGCATAGACCGCATTTATCCGCC
GGTCAACAAAAACCTTGCCTATGAAATCGCCGAAAAAGGATTGATTGTCAGCGAGTTCCCCATCGGCACGCGGCCGTATG
CCGGCAATTTTCCGCGCCGCAACCGCCTGATTGCCGCCCTGTCGCAAGTAACGCTGGTGGTTGAAGCCGCGTTGGAATCC
GGTTCGCTGATTACTGCCAGATTGGCGGCGGAGATGGGGCGCGAAGTGATGGCGGTACCCGGCTCGATAGACAATCCACA
CAGTAAAGGCTGCCACAAACTGATTAAAGACGGCGCAAAATTGGTGGAATGCCTGGACGACATCCTGAACGAATGCCCGG
GGCTATTGCAAAATACGGGTGCTTCATCATATTCTATAAATAAGGGAATACCTGAAAAGCGCATCACTGCCGTTCAGACG
GCATCCGACCAGCTGTCTCTGCCTGAAGGCAAAATGCCGTCTGAAAAGACGGAGAACCGACCCGTCGGCGGCAGTATCTT
GGACAGGATGGGTTTCGACCCAGTTCATCCCGACGTGCTTGCCGGACAGTTGGCTATGCCTGCCGCAGATTTGTATGCCG
CACTGTTGGAATTGGAATTGGACGGCAGCGTTGCCGCAATGCCCGGCGGCAGATACCAGCGTATCCGAACTTGA

Domains

Predicted by InterproScan.

DNA recombination-mediator protein A (DNA_processg_A)

(79-283)

DprA winged helix domain (DprA_WH)

(344-390)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9K1K1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Neisseria meningitidis MC58

100

100

1
  dprA Neisseria gonorrhoeae strain FA1090

90.176

100

0.906
  dprA Neisseria gonorrhoeae MS11

89.924

100

0.904
  dprA Vibrio cholerae strain A1552

37.845

100

0.407
  dprA Vibrio campbellii strain DS40M4

37.084

100

0.393

Multiple sequence alignment    



References

[1] Yao-Hui Sun et al. (2005) Identification and characterization of genes required for competence in Neisseria meningitidis. Journal of Bacteriology 187(9):3273-6. [PMID: 15838056]