Detailed information    

experimental Experimentally validated

Overview

Name   dprA   Type   Machinery gene
Locus tag   OK783_RS09595 Genome accession   NZ_CP115654
Coordinates   1835852..1837039 (-) Length   395 a.a.
NCBI ID   WP_003705341.1    Uniprot ID   Q5F5Q2
Organism   Neisseria gonorrhoeae strain FA1090     
Function   ssDNA binding; loading RecA onto ssDNA   
DNA processing

Function

Inactivation of dprA completely abrogated transformation of gyrB1-encoding DNA. Loss of transformation in the dprA null mutants was independent of the DNA uptake sequence. DprA mutants exhibited increased RecA-dependent pilin variation suggesting that DprA affects pilin variation.


Genomic Context

Location: 1830852..1842039
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OK783_RS09575 (OK783_09575) - 1831160..1831996 (-) 837 WP_164920418.1 opacity family porin -
  OK783_RS09580 (OK783_09580) - 1832098..1832894 (-) 797 Protein_1836 IS1595 family transposase -
  OK783_RS09585 (OK783_09585) topA 1832981..1835287 (-) 2307 WP_003705339.1 type I DNA topoisomerase -
  OK783_RS09590 (OK783_09590) - 1835365..1835826 (-) 462 WP_003690120.1 DUF494 domain-containing protein -
  OK783_RS09595 (OK783_09595) dprA 1835852..1837039 (-) 1188 WP_003705341.1 DNA-processing protein DprA Machinery gene
  OK783_RS09600 (OK783_09600) - 1837130..1838407 (-) 1278 WP_003690124.1 sigma-54-dependent transcriptional regulator -
  OK783_RS09605 (OK783_09605) - 1838400..1840520 (-) 2121 WP_003690126.1 HAMP domain-containing histidine kinase -
  OK783_RS09610 (OK783_09610) - 1840520..1841119 (-) 600 WP_003690128.1 DUF4390 domain-containing protein -

Sequence

Protein


Download         Length: 395 a.a.        Molecular weight: 41852.92 Da        Isoelectric Point: 6.3096

>NTDB_id=1122 OK783_RS09595 WP_003705341.1 1835852..1837039(-) (dprA) [Neisseria gonorrhoeae strain FA1090]
MTEDERFAWLQLAFTPYIGAESFLLLMRSFGSAQNALSAPAEQVAPAVRHKHALEAWRNAEKRALARQAAEAALEWEMRD
GCRLMLLQDEDFPEMLTQGLTAPPVLFLRGNVRLLHKPSAAIVGSRHATPQAMRIAKDFGRALGGKGIPTVSGMASGIDT
AAHQGALEAEGGTVAVWGTGIDRIYPPANKNLAYEIAEKGLIVSEFPIGTRPYAGNFPRRNRLIAALSQVTLVVEAALES
GSLITAGLAAEMGREVMAVPGSIDNPHSKGCHKLIKDGAKLTECLDDILNECPGLLQNTGASSYSINKDTPDTGRRAVQT
AYAPPPAAKMPSEGAAGGTAVGGILDKMGFDPVHPDVLAGQLAMPAADLYAALLELELDGSVAAMPGGRYQRIRT

Nucleotide


Download         Length: 1188 bp        

>NTDB_id=1122 OK783_RS09595 WP_003705341.1 1835852..1837039(-) (dprA) [Neisseria gonorrhoeae strain FA1090]
ATGACAGAGGACGAACGTTTCGCGTGGCTGCAATTGGCGTTTACGCCCTATATCGGCGCGGAAAGTTTCCTGCTGCTGAT
GCGCAGTTTCGGCAGCGCGCAAAATGCCTTGTCCGCACCGGCGGAACAGGTTGCCCCGGCGGTGCGGCATAAGCACGCGC
TTGAGGCTTGGCGCAATGCGGAAAAACGCGCTTTGGCGCGGCAGGCGGCAGAAGCGGCGTTGGAATGGGAAATGCGGGAC
GGATGCCGCCTGATGCTGCTTCAGGATGAAGATTTCCCCGAAATGCTGACGCAGGGGCTGACCGCGCCGCCGGTTTTGTT
TTTGCGCGGCAACGTGCGGCTGCTGCACAAGCCTTCCGCCGCCATCGTCGGCAGCCGCCATGCCACGCCGCAGGCGATGC
GGATTGCCAAAGATTTCGGCAGGGCGTTGGGTGGGAAAGGCATTCCTACCGTGTCGGGTATGGCTTCGGGCATCGACACC
GCCGCCCATCAGGGCGCACTGGAGGCAGAAGGCGGCACCGTCGCCGTGTGGGGGACGGGCATAGACCGCATTTATCCGCC
GGCCAACAAAAACCTTGCCTATGAAATCGCCGAAAAAGGGCTGATTGTCAGCGAGTTCCCCATCGGCACGCGGCCGTATG
CCGGCAACTTTCCGCGCCGCAACCGCCTGATTGCCGCGCTGTCGCAGGTTACGCTGGTGGTCGAGGCCGCCTTGGAATCC
GGTTCGCTGATTACCGCCGGCCTGGCGGCAGAAATGGGGCGCGAAGTGATGGCGGTACCCGGTTCGATAGACAACCCCCA
CAGCAAAGGCTGCCACAAACTGATTAAAGACGGAGCGAAACTGACGGAATGTTTGGACGACATCCTGAACGAATGCCCGG
GGCTATTGCAAAATACGGGTGCTTCGTCATATTCTATAAATAAGGATACGCCCGACACGGGCCGCCGCGCCGTTCAGACG
GCATACGCCCCGCCGCCTGCCGCAAAAATGCCGTCTGAAGGGGCGGCAGGCGGCACAGCCGTCGGCGGCATACTGGATAA
AATGGGTTTCGACCCGGTTCATCCCGACGTGCTTGCCGGACAGTTGGCTATGCCTGCCGCAGATTTGTATGCCGCACTGT
TGGAATTGGAATTGGACGGCAGCGTTGCCGCAATGCCCGGCGGCAGATACCAGCGTATCCGAACTTGA

Domains

Predicted by InterproScan.

DNA recombination-mediator protein A (DNA_processg_A)

(79-282)

DprA winged helix domain (DprA_WH)

(341-388)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q5F5Q2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Neisseria gonorrhoeae MS11

99.241

100

0.992
  dprA Neisseria meningitidis MC58

90.176

100

0.906
  dprA Neisseria meningitidis strain C311

90.176

100

0.906
  dprA Vibrio cholerae strain A1552

36.658

100

0.396
  dprA Vibrio campbellii strain DS40M4

35.917

100

0.377

Multiple sequence alignment    



References

[1] Paul M Duffin et al. (2016) DprA is required for natural transformation and affects pilin variation in Neisseria gonorrhoeae. Microbiology (Reading, England) 162(9):1620-1628. [PMID: 27469477]