Detailed information    

experimental Experimentally validated

Overview

Name   dprA   Type   Machinery gene
Locus tag   A4U84_RS04945 Genome accession   NZ_CP015099
Coordinates   1080898..1082025 (+) Length   375 a.a.
NCBI ID   WP_062924099.1    Uniprot ID   -
Organism   Glaesserella parasuis strain SC1401     
Function   ssDNA binding; loading RecA onto ssDNA   
DNA processing

Function

The expression levels of candidate uptake components were significantly lower than in SC1401, respectively. These results revealed that the highly naturally competent strain SC1401 up-regulated most of the candidate natural competence-related regulons/components.


Genomic Context

Location: 1075898..1087025
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A4U84_RS04925 (A4U84_04925) - 1077354..1078295 (-) 942 WP_021111453.1 hypothetical protein -
  A4U84_RS04930 (A4U84_04930) rfaD 1078387..1079313 (-) 927 WP_062924098.1 ADP-glyceromanno-heptose 6-epimerase -
  A4U84_RS04935 (A4U84_04935) def 1079325..1079837 (-) 513 WP_005712670.1 peptide deformylase -
  A4U84_RS04940 (A4U84_04940) - 1080055..1080873 (+) 819 WP_021114360.1 BRO-N domain-containing protein -
  A4U84_RS04945 (A4U84_04945) dprA 1080898..1082025 (+) 1128 WP_062924099.1 DNA-processing protein DprA Machinery gene
  A4U84_RS04950 (A4U84_04950) - 1082078..1082914 (+) 837 WP_081120185.1 heme/hemin ABC transporter substrate-binding protein -
  A4U84_RS04955 (A4U84_04955) - 1082914..1083900 (+) 987 WP_062924100.1 FecCD family ABC transporter permease -
  A4U84_RS04960 (A4U84_04960) - 1083900..1084688 (+) 789 WP_062924101.1 heme ABC transporter ATP-binding protein -
  A4U84_RS04965 (A4U84_04965) - 1085379..1086227 (+) 849 WP_062924102.1 ESPR-type extended signal peptide-containing protein -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 41782.77 Da        Isoelectric Point: 5.4146

>NTDB_id=1388 A4U84_RS04945 WP_062924099.1 1080898..1082025(+) (dprA) [Glaesserella parasuis strain SC1401]
MTHLTKLLALLQVPQLGAQRIGRLLSEVDFGEFCQYDKTALKQMGWNERQIQRWFNPEHKWIDQALQWAEQDQQQILTLF
DEDYPFLLKQISTAPPVLFVRGNVESLSLPQIAIVGSRDYSHYGEHWAGHFSAELVKHQLAVTSGLAIGIDGFCHQRAAN
ENGITIAVLGSGLDQVYPARHKKLAEQIVETSGALVSEFFPSQPPIAENFPRRNRIISGLSLGTLVIEATVNSGSLITAR
YALEQGREVFALPNSVQNPYSEGCHKLIKEGALLVENVEDILEAIAWQRQSVHIQPSLFDTKPKQAVKSEPKFAKNLPEL
TACQQQLFEQISLEPISVDDLAKATDMAVETALIELLNLELLGVVKQVRGGYVIT

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=1388 A4U84_RS04945 WP_062924099.1 1080898..1082025(+) (dprA) [Glaesserella parasuis strain SC1401]
ATGACACATCTCACAAAATTATTAGCCCTGTTACAAGTGCCACAGCTTGGCGCGCAACGTATCGGGCGGTTACTCTCGGA
AGTGGACTTTGGCGAATTTTGTCAATACGACAAAACCGCATTAAAGCAAATGGGCTGGAATGAACGGCAAATTCAGCGTT
GGTTTAATCCTGAGCATAAATGGATTGATCAGGCGTTACAATGGGCAGAACAGGATCAGCAACAGATTTTAACCTTATTT
GACGAAGATTATCCTTTTTTATTGAAGCAGATCAGTACTGCTCCCCCTGTGCTTTTTGTGCGTGGGAATGTTGAAAGTTT
ATCGTTACCGCAGATAGCTATCGTCGGCAGTCGAGATTACTCCCACTATGGCGAACACTGGGCAGGGCATTTTTCGGCGG
AATTAGTGAAACATCAACTTGCTGTCACTAGTGGTTTAGCCATAGGCATAGACGGATTTTGTCATCAGCGTGCGGCCAAT
GAAAACGGGATTACTATTGCGGTTTTAGGCAGTGGTTTAGACCAAGTTTACCCTGCTCGCCATAAAAAACTTGCTGAACA
AATCGTTGAAACAAGCGGTGCGTTAGTGTCGGAATTTTTCCCTAGCCAGCCACCGATTGCTGAGAATTTTCCTAGACGAA
ATCGTATTATCAGCGGATTATCGCTCGGAACATTGGTTATTGAAGCAACAGTGAATAGTGGCTCGCTCATTACTGCCCGT
TATGCGTTAGAGCAAGGACGAGAAGTTTTTGCATTGCCGAACTCAGTACAAAACCCATATAGCGAAGGGTGCCATAAATT
GATTAAAGAAGGAGCATTACTGGTTGAAAATGTTGAAGATATTTTAGAAGCGATTGCGTGGCAACGGCAGTCAGTGCATA
TTCAACCAAGTCTATTTGATACGAAACCTAAACAAGCGGTGAAATCCGAACCCAAATTTGCAAAAAATCTACCTGAACTG
ACCGCTTGTCAGCAACAATTATTTGAACAGATTAGCCTTGAGCCGATCAGCGTTGATGATCTTGCCAAAGCAACAGATAT
GGCGGTAGAAACCGCATTAATTGAGCTGTTAAATTTGGAGTTGTTGGGTGTTGTGAAGCAGGTGAGAGGGGGGTATGTAA
TCACTTGA

Domains

Predicted by InterproScan.

DNA recombination-mediator protein A (DNA_processg_A)

(69-276)

DprA winged helix domain (DprA_WH)

(313-371)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Haemophilus influenzae Rd KW20

52.011

93.298

0.485
  dprA Vibrio campbellii strain DS40M4

47.185

100

0.477
  dprA Vibrio cholerae strain A1552

43.883

100

0.445
  dprA Legionella pneumophila strain ERS1305867

39.418

100

0.413
  dprA Neisseria gonorrhoeae MS11

42.994

83.733

0.36

Multiple sequence alignment    



References

[1] Ke Dai et al. (2018) Basic Characterization of Natural Transformation in a Highly Transformable Haemophilus parasuis Strain SC1401. Frontiers in Cellular And Infection Microbiology 8:32. [PMID: 29473023]