Detailed information NTG0001250

Experimentally validated

Overview

• Name: dprA
• Type: Machinery gene
• Locus tag: GCO85_RS13065
• Genome accession: NZ_CP048618
• Coordinates: 2932846..2933931 (+)
• Length: 361 a.a.
• NCBI ID: WP_027219989.1
• Uniprot ID: -
• Organism: Legionella pneumophila strain ERS1305867
• Function: ssDNA binding; loading RecA onto ssDNA DNA processing

Function

Mutation of this gene caused a decrease in transformation.

Genomic Context

Location: 2927846..2938931

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GCO85_RS13040 (GCO85_13050)	-	2928239..2928988 (-)	750	WP_014842652.1	CPBP family glutamic-type intramembrane protease	-
GCO85_RS13045 (GCO85_13055)	rsmB	2928985..2930268 (-)	1284	WP_061637074.1	16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB	-
GCO85_RS13050 (GCO85_13060)	fmt	2930265..2931209 (-)	945	WP_015961698.1	methionyl-tRNA formyltransferase	-
GCO85_RS13055 (GCO85_13065)	def	2931206..2931718 (-)	513	WP_010948295.1	peptide deformylase	-
GCO85_RS13060 (GCO85_13070)	-	2931812..2932849 (+)	1038	WP_014842655.1	LysM domain-containing protein	-
GCO85_RS13065 (GCO85_13075)	dprA	2932846..2933931 (+)	1086	WP_027219989.1	DNA-processing protein DprA	Machinery gene
GCO85_RS13070 (GCO85_13080)	-	2933921..2934358 (+)	438	WP_011947522.1	DUF494 family protein	-
GCO85_RS13075 (GCO85_13085)	topA	2934438..2936717 (+)	2280	WP_011947523.1	type I DNA topoisomerase	-
GCO85_RS13080 (GCO85_13090)	-	2936928..2938901 (-)	1974	WP_027219990.1	acyltransferase family protein	-

Sequence

Protein

• Length: 361 a.a.
• Molecular weight: 39541.68 Da
• Isoelectric Point: 7.2591

>NTDB_id=1250 GCO85_RS13065 WP_027219989.1 2932846..2933931(+) (dprA) [Legionella pneumophila strain ERS1305867]
MNNLQPLLALNRMKRIGPRTVLKLQKRWPDLNLMFQLSSVDLEEAGLPSWLAQTIKNFDPDFIKTDLNWLSSSENHHILT
WDSPYYPALLKEIADPPFILYAKGELSALTQPSLAIVGSRNASVTGNENARAFSREISRHGVSIVSGLALGIDAHAHLGC
LEGGGKTIAVLGTGIDCIYPRRHLKLAEQITENGLLLSEFPLKSPPIAGHFPLRNRIISGLSLCILVIEAAIKSGSLITA
RMALEQNRDVLAIPGSIHNPLARGCHYLLQQGAKLVTSVADVLDELKIEHHQFTSNKPIFSLASGKENLVKFIGFETTTI
DQIIDRSGYGMEQVTSGLAELELKGAVIAVPGGYIRCEYER

Nucleotide

• Length: 1086 bp

>NTDB_id=1250 GCO85_RS13065 WP_027219989.1 2932846..2933931(+) (dprA) [Legionella pneumophila strain ERS1305867]
ATGAATAATCTGCAACCCCTCCTCGCCCTAAACAGGATGAAAAGAATTGGCCCCCGAACCGTATTAAAACTGCAAAAAAG
ATGGCCTGATTTAAACCTTATGTTCCAACTGTCCAGTGTTGATCTGGAAGAAGCGGGCTTGCCATCCTGGTTGGCACAAA
CCATAAAAAATTTTGATCCGGACTTTATAAAAACTGATTTGAACTGGCTAAGTTCATCAGAAAATCATCATATTTTAACT
TGGGACTCCCCCTATTATCCCGCATTGCTCAAAGAAATAGCCGATCCTCCCTTCATTTTATACGCTAAAGGTGAGCTGTC
CGCCTTGACACAACCCAGCCTGGCTATTGTTGGAAGTCGCAATGCTTCTGTAACAGGAAACGAGAATGCCCGAGCGTTCT
CCAGGGAGATTTCCAGGCATGGGGTAAGCATAGTGAGTGGTTTAGCCTTGGGAATTGATGCTCATGCTCATTTAGGCTGT
CTGGAAGGTGGCGGCAAAACGATAGCCGTCTTAGGTACCGGAATTGATTGTATCTATCCGCGCCGGCATTTAAAATTAGC
TGAGCAAATTACAGAGAATGGTTTATTATTAAGCGAATTTCCTTTAAAAAGTCCACCAATTGCTGGACATTTTCCACTCA
GAAATCGTATAATAAGCGGTCTATCATTATGCATTTTGGTTATCGAGGCAGCAATTAAAAGCGGTTCTTTAATTACAGCA
AGAATGGCTCTGGAACAAAACAGAGATGTATTGGCTATTCCCGGATCGATTCATAACCCGTTAGCCAGGGGCTGCCACTA
CTTATTGCAGCAGGGTGCGAAATTAGTAACTTCAGTGGCTGATGTTCTTGATGAGCTAAAAATTGAGCACCATCAGTTCA
CTTCTAATAAACCAATTTTTTCCCTTGCCAGCGGTAAGGAAAACCTAGTAAAGTTCATTGGTTTTGAAACTACGACTATC
GATCAAATCATTGATCGTAGCGGATATGGCATGGAACAAGTCACGAGTGGACTGGCTGAACTAGAATTGAAAGGGGCTGT
GATAGCAGTGCCCGGTGGCTATATAAGGTGTGAATATGAAAGATAA

Domains

Predicted by InterproScan.

• DNA recombination-mediator protein A (DNA_processg_A) (74-277)

• DprA winged helix domain (DprA_WH) (300-353)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
dprA	Vibrio cholerae strain A1552	42.895	100	0.443
dprA	Vibrio campbellii strain DS40M4	46.505	91.136	0.424
dprA	Glaesserella parasuis strain SC1401	39.418	100	0.413
dprA	Bacillus subtilis subsp. subtilis str. 168	38.462	100	0.387
dprA	Streptococcus mutans UA159	44.77	85.357	0.382
dprA	Lactococcus lactis subsp. cremoris KW2	38.768	97.872	0.379
dprA	Haemophilus influenzae Rd KW20	40.634	93.029	0.378

References

• [1]: Léo Hardy et al. (2021) Transposon Insertion Sequencing in a Clinical Isolate of Legionella pneumophila Identifies Essential Genes and Determinants of Natural Transformation. Journal of Bacteriology 203(3):e00548-20. [PMID: 33168636]