Detailed information    

experimental Experimentally validated

Overview

Name   dprA   Type   Machinery gene
Locus tag   SMU_RS04605 Genome accession   NC_004350
Coordinates   945912..946754 (+) Length   280 a.a.
NCBI ID   WP_002262865.1    Uniprot ID   -
Organism   Streptococcus mutans UA159     
Function   ssDNA binding; loading RecA onto ssDNA   
DNA processing

Function

Deletion of the genes for recombinase A, recA, DNA processing protein, dprA and single-stranded DNA-binding protein, ssbA, produced results comparable with those from other bacteria, supporting the contention that these proteins have similar functions in S. mutans competence.


Genomic Context

Location: 940912..951754
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SMU_RS04580 (SMU.995) - 941595..942557 (+) 963 WP_002262859.1 ABC transporter permease -
  SMU_RS04585 (SMU.996) - 942554..943525 (+) 972 WP_002262860.1 iron chelate uptake ABC transporter family permease subunit -
  SMU_RS04590 (SMU.997) - 943522..944328 (+) 807 WP_002262861.1 ABC transporter ATP-binding protein -
  SMU_RS04595 (SMU.998) - 944376..945392 (+) 1017 WP_002262862.1 siderophore ABC transporter substrate-binding protein -
  SMU_RS04600 (SMU.999) - 945464..945745 (+) 282 WP_002262863.1 hypothetical protein -
  SMU_RS04605 (SMU.1001) dprA 945912..946754 (+) 843 WP_002262865.1 DNA-processing protein DprA Machinery gene
  SMU_RS04610 (SMU.1002) topA 946849..948966 (+) 2118 WP_002262866.1 type I DNA topoisomerase -
  SMU_RS04615 (SMU.1003) trmFO 949332..950666 (+) 1335 WP_002262867.1 methylenetetrahydrofolate--tRNA-(uracil(54)- C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  irvR dprA negative effect
  irvR comYC negative effect
  irvR comYF negative effect
  irvR comYG negative effect
  irvR negative effect
  irvR comYD negative effect
  irvR comYB negative effect
  irvR comYE negative effect
  irvR comYH negative effect
  irvR comYI negative effect
  irvR comYA negative effect

Sequence

Protein


Download         Length: 280 a.a.        Molecular weight: 31332.27 Da        Isoelectric Point: 9.1954

>NTDB_id=407 SMU_RS04605 WP_002262865.1 945912..946754(+) (dprA) [Streptococcus mutans UA159]
MDNFQLFKLKKAGLTNLNILNIIDYEERTQKSLSLRDMAVVSKNKKPLIFMEHYKNLDSKALRKEFNRFPSLSILDKEYP
LELKNIYNPPVLLFYQGDLDLLARPKLAVVGSRNASQMGVAAVKKIIQDLSKQFVIISGLARGIDTAAHLASLKSGGATI
AVIGTGLDVHYPKENRRLQDYIAKNHLLLSEYEAQSQPLKYHFPERNRIIAGLSQGVMVAEAKIRSGSLITCERAMEEGR
DVFVVPGNILDGQSEGCHHLIQEGAKCITSGFDILNEFNF

Nucleotide


Download         Length: 843 bp        

>NTDB_id=407 SMU_RS04605 WP_002262865.1 945912..946754(+) (dprA) [Streptococcus mutans UA159]
ATGGATAATTTTCAACTATTTAAACTTAAAAAAGCTGGTCTAACAAATCTGAATATCTTAAATATTATTGATTATGAAGA
GAGGACACAAAAATCCTTGTCTCTTCGTGATATGGCTGTTGTTTCCAAAAATAAAAAGCCTTTAATTTTTATGGAACATT
ACAAAAATCTTGATAGCAAAGCATTACGCAAAGAATTTAATCGTTTTCCTTCTTTATCCATTTTGGACAAAGAATATCCC
TTGGAACTTAAAAATATTTATAATCCGCCTGTTCTTCTATTTTATCAGGGAGACTTAGACTTGCTAGCTCGGCCTAAATT
AGCAGTAGTTGGTTCTCGCAACGCCAGTCAAATGGGAGTAGCTGCTGTTAAAAAGATCATACAAGACTTAAGTAAGCAAT
TTGTTATTATTAGCGGTTTAGCGCGTGGTATTGATACTGCAGCACATTTAGCAAGTCTTAAAAGCGGTGGAGCAACAATT
GCGGTCATCGGTACTGGACTAGATGTTCATTATCCTAAAGAAAATCGAAGACTGCAGGATTATATTGCAAAGAATCATTT
GCTCTTATCAGAATATGAAGCACAGAGTCAACCTCTTAAGTATCATTTTCCTGAGCGGAACCGCATTATTGCTGGGTTAT
CTCAAGGTGTTATGGTAGCAGAAGCAAAAATTCGTTCGGGTAGTCTGATTACTTGTGAACGTGCTATGGAAGAAGGACGC
GATGTTTTTGTTGTACCAGGAAATATTTTAGACGGCCAGTCAGAAGGATGCCATCACCTGATTCAAGAAGGAGCAAAATG
CATTACATCAGGCTTTGATATTTTAAATGAGTTTAACTTTTAA

Domains

Predicted by InterproScan.

DNA recombination-mediator protein A (DNA_processg_A)

(72-269)

DNA processing protein A sterile alpha motif domain (SAM_DprA)

(1-62)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA/cilB/dalA Streptococcus pneumoniae TIGR4

63.214

100

0.632
  dprA/cilB/dalA Streptococcus pneumoniae D39

63.214

100

0.632
  dprA/cilB/dalA Streptococcus pneumoniae R6

63.214

100

0.632
  dprA/cilB/dalA Streptococcus pneumoniae Rx1

63.214

100

0.632
  dprA/cilB/dalA Streptococcus mitis SK321

63.214

100

0.632
  dprA/cilB/dalA Streptococcus mitis NCTC 12261

62.857

100

0.629
  dprA Lactococcus lactis subsp. cremoris KW2

58.273

99.286

0.579
  dprA Legionella pneumophila strain ERS1305867

44.77

85.357

0.382
  dprA Staphylococcus aureus N315

38.258

94.286

0.361

Multiple sequence alignment    



References

[1] R E Eaton et al. (2010) Deletion of competence-induced genes over-expressed in biofilms caused transformation deficiencies in Streptococcus mutans. Molecular Oral Microbiology 25(6):406-17. [PMID: 21040514]