Detailed information    

experimental Experimentally validated

Overview

Name   irvR   Type   Regulator
Locus tag   SMU_RS06370 Genome accession   NC_004350
Coordinates   1326838..1327731 (+) Length   297 a.a.
NCBI ID   WP_002262721.1    Uniprot ID   A0A090B2L0
Organism   Streptococcus mutans UA159     
Function   regulate expression of competence genes   
Competence regulation

Function

Mutation of SMU.1398 (irvR) abolished genetic competence and reduced the expression of the late competence genes/operons comEA, comY, and dprA without affecting the expression of the known competence regulators comC, comED, or comX.


Genomic Context

Location: 1321838..1332731
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SMU_RS10140 (SMU.1395c) - 1323801..1323929 (-) 129 WP_002262718.1 hypothetical protein -
  SMU_RS10070 - 1323964..1324194 (-) 231 WP_002282891.1 hypothetical protein -
  SMU_RS06360 (SMU.1396) - 1324324..1326075 (-) 1752 WP_002262719.1 glucan-binding protein GbpC -
  SMU_RS06365 (SMU.1397c) - 1326238..1326523 (-) 286 Protein_1240 helix-turn-helix transcriptional regulator -
  SMU_RS06370 (SMU.1398) irvR 1326838..1327731 (+) 894 WP_002262721.1 LexA family transcriptional regulator IrvR Regulator
  SMU_RS06375 (SMU.1400c) - 1328400..1328777 (-) 378 WP_002263544.1 type II toxin-antitoxin system HicB family antitoxin -
  SMU_RS06380 - 1328795..1328986 (-) 192 WP_002263545.1 type II toxin-antitoxin system HicA family toxin -
  SMU_RS06385 (SMU.1402c) csn2 1329083..1329745 (-) 663 WP_002263546.1 type II-A CRISPR-associated protein Csn2 -
  SMU_RS06390 (SMU.1403c) cas2 1329735..1330079 (-) 345 WP_002263547.1 CRISPR-associated endonuclease Cas2 -
  SMU_RS06395 (SMU.1404c) cas1 1330076..1330942 (-) 867 WP_002352326.1 type II CRISPR-associated endonuclease Cas1 -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  irvR comYC negative effect
  irvR comYF negative effect
  irvR comYG negative effect
  irvR negative effect
  irvR comYD negative effect
  irvR dprA negative effect
  irvR comYB negative effect
  irvR comYE negative effect
  irvR comYH negative effect
  irvR comYI negative effect
  irvR comYA negative effect

Sequence

Protein


Download         Length: 297 a.a.        Molecular weight: 33651.40 Da        Isoelectric Point: 6.7038

>NTDB_id=405 SMU_RS06370 WP_002262721.1 1326838..1327731(+) (irvR) [Streptococcus mutans UA159]
MGRGKLTPQDLEAMKAISANLQRLLAESGMKQSHLATILNIPTSSLNEYVKGKSLPKIGNIQKIADYFGLQKSDIDPRFA
SKKDSSTLDKINHIAKQLEMKRQIKVLDFCYQQSKEQDETASLSASDETTEDKVADKSNHTQYQKVVDSFIAKELNDQTI
IHWDNVIKAKDLYQTFGRQYENIQIYGEVSAGTGIWVGHEKQETIKYPIPIPEHDIALIVNGNSMEPLFYNGDVIFVKKT
KAIHHGQIIVVIVNNEAYVKKLYRKNKEIRLISLNPDYDDIILKEDDTIEVIGTVIT

Nucleotide


Download         Length: 894 bp        

>NTDB_id=405 SMU_RS06370 WP_002262721.1 1326838..1327731(+) (irvR) [Streptococcus mutans UA159]
ATGGGACGCGGTAAATTAACTCCACAAGATTTAGAAGCAATGAAGGCTATTTCTGCCAATCTTCAAAGACTTCTAGCTGA
GAGCGGAATGAAACAATCTCACTTAGCTACTATTCTTAACATTCCTACAAGTAGTCTTAACGAATATGTTAAGGGAAAGA
GTTTGCCAAAAATAGGGAATATTCAAAAAATCGCAGATTACTTTGGTTTACAAAAATCCGATATTGACCCTAGATTTGCA
AGTAAAAAGGACTCTTCTACTCTTGATAAAATTAATCATATTGCCAAACAATTAGAAATGAAGAGACAGATAAAAGTCTT
AGATTTCTGTTACCAACAATCAAAAGAACAAGATGAAACAGCAAGCTTGTCTGCCAGTGATGAAACGACTGAGGATAAAG
TTGCTGACAAATCAAATCATACGCAGTATCAAAAGGTTGTTGATTCTTTTATTGCCAAAGAATTAAACGATCAGACAATT
ATTCATTGGGATAATGTGATAAAAGCTAAGGATTTGTATCAAACCTTTGGACGTCAATACGAAAACATTCAGATTTATGG
TGAAGTTTCAGCTGGTACTGGTATCTGGGTTGGACATGAAAAACAAGAAACCATTAAATACCCAATACCAATTCCAGAAC
ATGATATTGCTCTTATTGTCAATGGTAATAGTATGGAACCTTTATTTTACAACGGTGACGTTATTTTTGTTAAAAAGACT
AAAGCTATCCATCACGGACAAATTATCGTTGTTATTGTAAATAATGAGGCATATGTCAAAAAACTCTACCGAAAAAACAA
AGAAATTCGACTCATCTCATTAAATCCAGATTATGATGACATCATTTTAAAAGAAGATGATACAATTGAAGTCATCGGAA
CGGTCATTACCTAA

Domains

Predicted by InterproScan.

Peptidase S24-like (Peptidase_S24)

(184-296)

Helix-turn-helix (HTH_3)

(22-70)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A090B2L0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Guoqing Niu et al. (2008) Characterization of irvR, a novel regulator of the irvA-dependent pathway required for genetic competence and dextran-dependent aggregation in Streptococcus mutans. Journal of Bacteriology 190(21):7268-74. [PMID: 18757533]