Detailed information    

experimental Experimentally validated

Overview

Name   dprA/cilB/dalA   Type   Machinery gene
Locus tag   SM12261_RS05565 Genome accession   NZ_CP028414
Coordinates   1075233..1076081 (-) Length   282 a.a.
NCBI ID   WP_000705318.1    Uniprot ID   -
Organism   Streptococcus mitis NCTC 12261     
Function   ssDNA binding; loading RecA onto ssDNA   
DNA processing

Function

Upregulated in response to CSP as measured by RNA-seq.


Genomic Context

Location: 1070233..1081081
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SM12261_RS05545 (SM12261_1080) - 1070836..1071492 (-) 657 WP_000461578.1 MmcQ/YjbR family DNA-binding protein -
  SM12261_RS05550 (SM12261_1081) - 1071535..1072167 (-) 633 WP_000638813.1 copper homeostasis protein CutC -
  SM12261_RS05555 (SM12261_1082) - 1072260..1072619 (-) 360 WP_001220352.1 YbaN family protein -
  SM12261_RS05560 (SM12261_1084) topA 1072876..1074963 (-) 2088 WP_000179664.1 type I DNA topoisomerase -
  SM12261_RS05565 (SM12261_1086) dprA/cilB/dalA 1075233..1076081 (-) 849 WP_000705318.1 DNA-processing protein DprA Machinery gene
  SM12261_RS05570 (SM12261_1087) - 1076169..1076858 (-) 690 WP_000643976.1 sugar phosphate nucleotidyltransferase -
  SM12261_RS05575 (SM12261_1088) - 1076870..1077748 (-) 879 WP_049500425.1 DMT family transporter -
  SM12261_RS05580 (SM12261_1089) licA 1077738..1078607 (-) 870 WP_000411223.1 choline kinase LicA -
  SM12261_RS05585 (SM12261_1090) - 1078624..1079646 (-) 1023 WP_000609878.1 ribitol-5-phosphate dehydrogenase -
  SM12261_RS05590 (SM12261_1091) - 1079651..1080358 (-) 708 WP_000638494.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -

Sequence

Protein


Download         Length: 282 a.a.        Molecular weight: 31047.67 Da        Isoelectric Point: 5.0589

>NTDB_id=497 SM12261_RS05565 WP_000705318.1 1075233..1076081(-) (dprA/cilB/dalA) [Streptococcus mitis NCTC 12261]
MKITNYEIYKLKKSGLTNQQVLAVLEYGENVDQELLLGDIAEISGCRNPAVFMERYFQIDDAHLEKEFQKFPSFSILDDC
YPWDLSEIYDAPVLLFYKGNLDLLKFPKVAVVGSRACSKQGAKSVEKVIQGLENELVIVSGLAKGIDTAAHMAALQNGGK
TIAVIGTGLDVFYPKANKRLQDYIGNDHLVLSEYGPGEQPLKFHFPARNRIIAGLCRGVIVAEAKMRSGSLITCERAMEE
GRDVFAIPGSILDGLSDGCHHLIQEGAKLVTSGQDVLAEFEF

Nucleotide


Download         Length: 849 bp        

>NTDB_id=497 SM12261_RS05565 WP_000705318.1 1075233..1076081(-) (dprA/cilB/dalA) [Streptococcus mitis NCTC 12261]
ATGAAAATCACAAACTATGAAATCTATAAATTGAAAAAATCAGGTTTGACCAATCAACAGGTTTTGGCAGTTCTAGAATA
CGGTGAAAATGTCGATCAAGAACTTTTGTTGGGTGATATTGCAGAAATATCAGGTTGCAGAAATCCAGCTGTTTTTATGG
AACGTTACTTTCAGATAGACGATGCGCATTTGGAGAAGGAGTTTCAAAAATTTCCATCTTTCTCTATTTTAGATGACTGT
TACCCTTGGGATTTGAGTGAAATATATGATGCGCCTGTACTTTTATTTTACAAGGGAAATCTTGACCTCTTGAAATTCCC
GAAGGTAGCAGTCGTGGGCAGTCGTGCGTGTAGCAAACAGGGAGCTAAGTCAGTTGAAAAAGTCATTCAGGGCTTGGAAA
ATGAACTGGTTATCGTCAGTGGCTTAGCCAAGGGCATTGACACAGCAGCTCATATGGCAGCTCTTCAGAATGGCGGAAAA
ACCATTGCAGTGATTGGAACAGGACTGGATGTGTTTTATCCTAAAGCCAATAAACGCTTGCAAGACTACATCGGCAATGA
CCATCTGGTTCTAAGTGAATACGGACCTGGTGAACAACCTCTGAAATTTCATTTTCCTGCCCGTAATCGTATCATTGCTG
GACTTTGTCGTGGTGTGATTGTAGCAGAGGCTAAGATGCGTTCAGGTAGTCTCATTACCTGTGAGAGAGCAATGGAAGAA
GGGCGCGATGTCTTTGCTATTCCTGGTAGCATTTTAGATGGACTATCAGACGGTTGCCATCATTTGATTCAAGAAGGGGC
AAAATTGGTCACCAGCGGGCAGGATGTTCTTGCGGAATTTGAATTTTAA

Domains

Predicted by InterproScan.

DNA processing protein A sterile alpha motif domain (SAM_DprA)

(3-64)

DNA recombination-mediator protein A (DNA_processg_A)

(75-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA/cilB/dalA Streptococcus pneumoniae D39

98.582

100

0.986
  dprA/cilB/dalA Streptococcus pneumoniae Rx1

98.582

100

0.986
  dprA/cilB/dalA Streptococcus pneumoniae R6

98.582

100

0.986
  dprA/cilB/dalA Streptococcus pneumoniae TIGR4

98.582

100

0.986
  dprA/cilB/dalA Streptococcus mitis SK321

97.163

100

0.972
  dprA Streptococcus mutans UA159

62.857

100

0.629
  dprA Lactococcus lactis subsp. cremoris KW2

55.714

99.291

0.553
  dprA Haemophilus influenzae Rd KW20

38.202

94.681

0.362

Multiple sequence alignment    



References

[1] G Salvadori et al. (2018) High-resolution profiles of the Streptococcus mitis CSP signaling pathway reveal core and strain-specific regulated genes. BMC Genomics 19(1):453. [PMID: 29898666]