Detailed information NTG0000525

Experimentally validated

Overview

• Name: dprA/cilB/dalA
• Type: Machinery gene
• Locus tag: SMSK321_RS06295
• Genome accession: NZ_AEDT01000016
• Coordinates: 33513..34361 (-)
• Length: 282 a.a.
• NCBI ID: WP_000705298.1
• Uniprot ID: -
• Organism: Streptococcus mitis SK321
• Function: ssDNA binding; loading RecA onto ssDNA DNA processing

Function

Upregulated in response to CSP as measured by RNA-seq.

Genomic Context

Location: 28513..39361

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SMSK321_RS06275 (SMSK321_0691)	-	29418..30074 (-)	657	WP_000461601.1	MmcQ/YjbR family DNA-binding protein	-
SMSK321_RS06280 (SMSK321_0692)	-	30118..30750 (-)	633	WP_000638766.1	copper homeostasis protein CutC	-
SMSK321_RS06285 (SMSK321_0693)	-	30843..31202 (-)	360	WP_001237216.1	YbaN family protein	-
SMSK321_RS06290 (SMSK321_0694)	topA	31308..33395 (-)	2088	WP_000179673.1	type I DNA topoisomerase	-
SMSK321_RS06295 (SMSK321_0695)	dprA/cilB/dalA	33513..34361 (-)	849	WP_000705298.1	DNA-processing protein DprA	Machinery gene
SMSK321_RS06300 (SMSK321_0696)	-	34450..35139 (-)	690	WP_000643978.1	sugar phosphate nucleotidyltransferase	-
SMSK321_RS06305 (SMSK321_0697)	-	35151..36029 (-)	879	WP_000791828.1	DMT family transporter	-
SMSK321_RS06310 (SMSK321_0698)	licA	36019..36888 (-)	870	WP_000411224.1	choline kinase LicA	-
SMSK321_RS06315 (SMSK321_0699)	-	36905..37927 (-)	1023	WP_000609878.1	ribitol-5-phosphate dehydrogenase	-
SMSK321_RS06320 (SMSK321_0700)	-	37932..38639 (-)	708	WP_000638492.1	IspD/TarI family cytidylyltransferase	-

Sequence

Protein

• Length: 282 a.a.
• Molecular weight: 31130.84 Da
• Isoelectric Point: 5.4157

>NTDB_id=525 SMSK321_RS06295 WP_000705298.1 33513..34361(-) (dprA/cilB/dalA) [Streptococcus mitis SK321]
MKITNYEIYKLKKSGLTNQQILKVLEYGENVDQELLLGDIADISGCRNPAVFMERYFQIDDAHLEKEFKKIPSFSILDDC
YPWDLSEIYDAPVLLFYKGNLDLLKFPKVAVVGSRSCSKQGAKSVEKIIQGLENELVIVSGLAKGIDTAAHMAALQNGGK
TIAVIGTGLDVFYPKANKRLQDYISNDHLVLSEYGPGEQPLKFHFPARNRIIAGLCRGVIVAEAKMRSGSLITCERAMEE
GRDVFAIPGSILDGLSDGCHHLIQEGAKLVTSGQDVLAEFEF

Nucleotide

• Length: 849 bp

>NTDB_id=525 SMSK321_RS06295 WP_000705298.1 33513..34361(-) (dprA/cilB/dalA) [Streptococcus mitis SK321]
ATGAAAATCACAAACTATGAAATCTATAAGTTAAAAAAATCAGGTTTGACCAATCAACAGATTTTGAAAGTCCTAGAATA
CGGTGAAAATGTTGATCAAGAACTGTTGTTAGGCGATATTGCAGATATATCAGGTTGCCGAAATCCAGCTGTTTTTATGG
AACGTTATTTTCAGATAGACGATGCGCATTTGGAGAAGGAGTTTAAAAAAATTCCATCTTTCTCTATTTTAGATGACTGT
TACCCTTGGGATTTGAGTGAAATATACGATGCGCCTGTACTTTTATTTTACAAGGGAAATCTAGATCTCTTGAAATTTCC
TAAGGTAGCAGTCGTAGGAAGTCGTTCTTGCAGCAAACAGGGAGCTAAGTCGGTTGAAAAAATTATTCAGGGATTGGAAA
ATGAACTGGTTATCGTCAGTGGATTAGCCAAGGGCATTGACACAGCAGCTCATATGGCAGCTCTCCAGAATGGCGGCAAA
ACTATTGCAGTGATTGGAACAGGACTGGATGTGTTTTATCCTAAAGCCAATAAACGCTTGCAAGACTATATCAGCAATGA
CCATCTGGTTCTTAGTGAATATGGACCTGGTGAACAACCTCTGAAATTTCATTTTCCTGCCCGTAATCGCATCATTGCTG
GACTTTGTCGTGGTGTGATTGTAGCAGAGGCTAAGATGCGCTCAGGTAGTCTCATTACCTGTGAGAGGGCCATGGAAGAA
GGACGCGATGTTTTTGCTATTCCTGGTAGCATTTTAGATGGACTATCAGACGGTTGCCATCATTTGATTCAAGAAGGGGC
AAAATTGGTCACCAGCGGGCAAGATGTTCTTGCGGAATTTGAATTTTAA

Domains

Predicted by InterproScan.

• DNA processing protein A sterile alpha motif domain (SAM_DprA) (3-64)

• DNA recombination-mediator protein A (DNA_processg_A) (74-271)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
dprA/cilB/dalA	Streptococcus pneumoniae D39	97.872	100	0.979
dprA/cilB/dalA	Streptococcus pneumoniae Rx1	97.872	100	0.979
dprA/cilB/dalA	Streptococcus pneumoniae R6	97.872	100	0.979
dprA/cilB/dalA	Streptococcus pneumoniae TIGR4	97.872	100	0.979
dprA/cilB/dalA	Streptococcus mitis NCTC 12261	97.163	100	0.972
dprA	Streptococcus mutans UA159	63.214	100	0.632
dprA	Lactococcus lactis subsp. cremoris KW2	56.071	99.291	0.557
dprA	Staphylococcus aureus N315	37.456	100	0.376

References

• [1]: G Salvadori et al. (2018) High-resolution profiles of the Streptococcus mitis CSP signaling pathway reveal core and strain-specific regulated genes. BMC Genomics 19(1):453. [PMID: 29898666]