Detailed information    

experimental Experimentally validated

Overview

Name   dprA   Type   Machinery gene
Locus tag   SA_RS06185 Genome accession   NC_002745
Coordinates   1236145..1237017 (+) Length   290 a.a.
NCBI ID   WP_000593194.1    Uniprot ID   A0A9P3DKN4
Organism   Staphylococcus aureus N315     
Function   ssDNA binding; loading RecA onto ssDNA   
DNA processing

Function

Similar to SsbB, DprA and RecA bind internalized ssDNA and protect it from degradation. DprA displaces SsbB and stimulates loading of RecA onto the ssDNA fragment.


Genomic Context

Location: 1231145..1242017
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SA_RS06165 (SA1088) sucC 1231259..1232425 (+) 1167 WP_001020801.1 ADP-forming succinate--CoA ligase subunit beta -
  SA_RS06170 (SA1089) sucD 1232447..1233355 (+) 909 WP_000110253.1 succinate--CoA ligase subunit alpha -
  SA_RS06175 (SA1090) - 1233582..1234700 (+) 1119 WP_001041666.1 LysM peptidoglycan-binding domain-containing protein -
  SA_RS06180 (SA1091) fmhC 1234728..1235972 (+) 1245 WP_000672869.1 FemA/FemB family glycyltransferase FmhC -
  SA_RS06185 (SA1092) dprA 1236145..1237017 (+) 873 WP_000593194.1 DNA-processing protein DprA Machinery gene
  SA_RS06190 (SA1093) topA 1237191..1239266 (+) 2076 WP_001557331.1 type I DNA topoisomerase -
  SA_RS06195 (SA1094) trmFO 1239422..1240729 (+) 1308 WP_000195254.1 methylenetetrahydrofolate--tRNA-(uracil(54)- C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  sigH dprA positive effect
  sigH comGD positive effect
  agrC comGD positive effect
  braS comGD positive effect
  braR comGD positive effect
  vraR comGD negative effect
  agrA comGD positive effect
  comK/comK1 comGD positive effect
  vraS comGD negative effect
  agrC comGB positive effect
  braR comGB positive effect
  sigH comGB positive effect
  braS comGB positive effect
  vraS comGB negative effect
  agrA comGB positive effect
  comK/comK1 comGB positive effect
  vraR comGB negative effect
  agrC comGC positive effect
  vraS comGC negative effect
  agrA comGC positive effect
  comK/comK1 comGC positive effect
  vraR comGC negative effect
  braR comGC positive effect
  sigH comGC positive effect
  braS comGC positive effect
  agrC comGE positive effect
  vraS comGE negative effect
  agrA comGE positive effect
  comK/comK1 comGE positive effect
  vraR comGE negative effect
  braR comGE positive effect
  sigH comGE positive effect
  braS comGE positive effect
  agrC comGA positive effect
  agrA comGA positive effect
  comK/comK1 comGA positive effect
  vraR comGA negative effect
  vraS comGA negative effect
  sigH comGA positive effect
  braS comGA positive effect
  braR comGA positive effect
  agrC comGF positive effect
  agrA comGF positive effect
  comK/comK1 comGF positive effect
  vraR comGF negative effect
  vraS comGF negative effect
  sigH comGF positive effect
  braS comGF positive effect
  braR comGF positive effect
  sigH coiA positive effect
  comK/comK1 coiA positive effect
  sigH positive effect
  comK/comK1 positive effect
  sigH comEA positive effect
  comK/comK1 comEA positive effect
  sigH ssb positive effect
  comK/comK1 ssb positive effect
  comK/comK1 dprA positive effect

Sequence

Protein


Download         Length: 290 a.a.        Molecular weight: 33541.01 Da        Isoelectric Point: 9.5093

>NTDB_id=25 SA_RS06185 WP_000593194.1 1236145..1237017(+) (dprA) [Staphylococcus aureus N315]
MIKLFLLKLYWAHFSTKQIHQFLMAYPNVIKEEGRKKDSYLCEWVNREENVLLLRKYYAFIKLDHNDIIKELQKLKVSYI
TYMDSEYPVLLKEIYQFPLLLFYKGNIKLINNMHHLAVVGARDSTSYTQQSLEFLLSNDKSKYLTIVSGLAQGADAMAHQ
IALKYNLPTIAVLAFGHQTHYPKSTLALRNKIEEKGLVISEYPPHTPIAKYRFPERNRIISGLSKGVLITEAKEQSGSHI
TIDFALEQNRNVYVLPGSMFNPMTKGNLLRIQEGAKVVLNANDIFEDYYI

Nucleotide


Download         Length: 873 bp        

>NTDB_id=25 SA_RS06185 WP_000593194.1 1236145..1237017(+) (dprA) [Staphylococcus aureus N315]
TTGATTAAACTATTTTTGCTTAAGTTATACTGGGCACACTTTTCGACTAAACAAATTCATCAATTTTTAATGGCATATCC
TAATGTAATTAAAGAGGAGGGAAGAAAAAAAGATAGTTATTTATGTGAATGGGTGAATAGGGAAGAAAATGTTCTTTTAT
TACGTAAATACTATGCTTTTATAAAACTTGATCATAACGATATTATTAAAGAACTGCAGAAATTAAAAGTAAGTTACATT
ACATATATGGATTCTGAATACCCAGTGCTATTAAAAGAAATATATCAATTTCCATTACTTCTTTTCTATAAAGGGAACAT
CAAATTAATAAATAATATGCATCATTTGGCAGTAGTAGGTGCAAGAGATTCTACAAGTTATACCCAACAGTCTTTAGAAT
TTTTATTATCAAATGATAAAAGCAAATATTTAACAATTGTTTCCGGCCTTGCTCAAGGAGCTGATGCAATGGCACATCAA
ATAGCTTTAAAATACAATCTCCCTACAATTGCAGTTTTAGCCTTTGGCCATCAAACACATTATCCCAAAAGTACATTAGC
ATTAAGAAATAAAATAGAAGAAAAAGGTTTAGTTATATCCGAATATCCACCACATACACCAATTGCTAAATATAGATTTC
CTGAGCGCAATAGAATTATCAGCGGTTTGTCAAAAGGGGTTTTAATTACTGAGGCTAAGGAACAAAGTGGCAGTCACATC
ACGATAGATTTTGCATTAGAGCAAAATAGAAATGTTTATGTTTTACCTGGATCTATGTTTAATCCTATGACAAAAGGTAA
TTTATTACGTATCCAAGAAGGTGCTAAGGTAGTATTAAACGCTAATGATATATTTGAAGACTACTATATTTAA

Domains

Predicted by InterproScan.

DNA recombination-mediator protein A (DNA_processg_A)

(72-279)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Staphylococcus aureus MW2

99.655

100

0.997
  dprA/cilB/dalA Streptococcus pneumoniae Rx1

37.809

100

0.379
  dprA/cilB/dalA Streptococcus pneumoniae D39

37.809

100

0.379
  dprA/cilB/dalA Streptococcus pneumoniae R6

37.809

100

0.379
  dprA/cilB/dalA Streptococcus pneumoniae TIGR4

37.809

100

0.379
  dprA/cilB/dalA Streptococcus mitis SK321

37.456

100

0.376
  dprA Streptococcus mutans UA159

38.258

94.286

0.361

Multiple sequence alignment    



References

[1] Annette Fagerlund et al. (2014) Staphylococcus aureus competence genes: mapping of the SigH, ComK1 and ComK2 regulons by transcriptome sequencing. Molecular Microbiology 94(3):557-79. [PMID: 25155269]