Detailed information    

experimental Experimentally validated

Overview

Name   comGC   Type   Machinery gene
Locus tag   SA_RS07765 Genome accession   NC_002745
Coordinates   1578832..1579143 (-) Length   103 a.a.
NCBI ID   WP_000472256.1    Uniprot ID   -
Organism   Staphylococcus aureus N315     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus   
DNA binding and uptake

Function

In addition, the staphylococcal comG operon contains a homologue to the gene encoding the major pilins of S. pneumoniae and B. subtilis (comGC) and three small open reading frames (comGD, comGE and comGF) encoding minor pilin subunits.


Genomic Context

Location: 1573832..1584143
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SA_RS07730 (SA1366) gcvPA 1574334..1575680 (-) 1347 WP_000019691.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  SA_RS07735 (SA1367) gcvT 1575700..1576791 (-) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  SA_RS07740 (SA1368) - 1576950..1577474 (-) 525 WP_001015120.1 shikimate kinase -
  SA_RS07745 - 1577464..1577610 (-) 147 WP_001792109.1 hypothetical protein -
  SA_RS07750 comGF 1577707..1578204 (-) 498 WP_001788897.1 competence type IV pilus minor pilin ComGF Machinery gene
  SA_RS07755 (SA1370) comGE 1578122..1578421 (-) 300 WP_000844410.1 hypothetical protein Machinery gene
  SA_RS07760 (SA1371) comGD 1578408..1578854 (-) 447 WP_001788899.1 competence type IV pilus minor pilin ComGD Machinery gene
  SA_RS07765 (SA1372) comGC 1578832..1579143 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  SA_RS07770 (SA1373) comGB 1579157..1580227 (-) 1071 WP_000776422.1 competence type IV pilus assembly protein ComGB Machinery gene
  SA_RS07775 (SA1374) comGA 1580199..1581173 (-) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  SA_RS07780 (SA1375) - 1581225..1581848 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  SA_RS07785 (SA1376) - 1581845..1582174 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  SA_RS07790 (SA1377) - 1582174..1583160 (-) 987 WP_000161311.1 ROK family glucokinase -
  SA_RS07795 (SA1378) - 1583157..1583360 (-) 204 WP_000087561.1 YqgQ family protein -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  vraS comGC negative effect
  agrA comGC positive effect
  comK/comK1 comGC positive effect
  vraR comGC negative effect
  braR comGC positive effect
  sigH comGC positive effect
  agrC comGC positive effect
  braS comGC positive effect
  vraS comGE negative effect
  agrA comGE positive effect
  comK/comK1 comGE positive effect
  vraR comGE negative effect
  braR comGE positive effect
  sigH comGE positive effect
  agrC comGE positive effect
  braS comGE positive effect
  vraS comGD negative effect
  agrC comGD positive effect
  braS comGD positive effect
  sigH comGD positive effect
  braR comGD positive effect
  vraR comGD negative effect
  agrA comGD positive effect
  comK/comK1 comGD positive effect
  vraS comGA negative effect
  agrA comGA positive effect
  comK/comK1 comGA positive effect
  vraR comGA negative effect
  sigH comGA positive effect
  agrC comGA positive effect
  braS comGA positive effect
  braR comGA positive effect
  vraS comGF negative effect
  agrA comGF positive effect
  comK/comK1 comGF positive effect
  vraR comGF negative effect
  sigH comGF positive effect
  agrC comGF positive effect
  braS comGF positive effect
  braR comGF positive effect
  vraS comGB negative effect
  braR comGB positive effect
  sigH comGB positive effect
  agrC comGB positive effect
  braS comGB positive effect
  agrA comGB positive effect
  comK/comK1 comGB positive effect
  vraR comGB negative effect
  comK/comK1 coiA positive effect
  sigH coiA positive effect
  comK/comK1 positive effect
  sigH positive effect
  comK/comK1 comEA positive effect
  sigH comEA positive effect
  comK/comK1 ssb positive effect
  sigH ssb positive effect
  comK/comK1 dprA positive effect
  sigH dprA positive effect

Sequence

Protein


Download         Length: 103 a.a.        Molecular weight: 11315.36 Da        Isoelectric Point: 8.5268

>NTDB_id=16 SA_RS07765 WP_000472256.1 1578832..1579143(-) (comGC) [Staphylococcus aureus N315]
MFKFLKKTQAFTLIEMLLVLLIISLLLILIIPNIAKQTAHIQSTGCNAQVKMVNSQIEAYALKHNRNPSSIEDLIADGFI
KEAQKTCKSGETITISNGEAVAN

Nucleotide


Download         Length: 312 bp        

>NTDB_id=16 SA_RS07765 WP_000472256.1 1578832..1579143(-) (comGC) [Staphylococcus aureus N315]
ATGTTTAAATTTCTTAAGAAAACTCAAGCGTTTACATTGATAGAGATGCTATTAGTGTTATTAATCATCAGTTTATTATT
AATTTTAATCATTCCAAATATTGCTAAACAAACTGCTCACATACAATCAACAGGTTGTAATGCACAGGTAAAAATGGTTA
ATAGTCAAATTGAAGCGTATGCATTGAAACATAATAGAAATCCATCGTCTATTGAAGACTTAATTGCAGATGGTTTTATA
AAAGAAGCACAAAAGACATGTAAATCAGGAGAGACAATAACAATTAGTAATGGAGAAGCAGTTGCAAATTAG

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(6-30)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGC Staphylococcus aureus MW2

100

100

1
  comGC/cglC Streptococcus pneumoniae R6

46.078

99.029

0.456
  comGC/cglC Streptococcus pneumoniae Rx1

46.078

99.029

0.456
  comGC/cglC Streptococcus pneumoniae TIGR4

46.078

99.029

0.456
  comGC/cglC Streptococcus pneumoniae D39

46.078

99.029

0.456
  comGC/cglC Streptococcus mitis SK321

51.163

83.495

0.427
  comGC/cglC Streptococcus mitis NCTC 12261

51.163

83.495

0.427
  comYC Streptococcus suis isolate S10

50

83.871

0.419
  comYC Streptococcus gordonii str. Challis substr. CH1

42.857

95.146

0.408
  comGC Bacillus subtilis subsp. subtilis str. 168

41.304

93.878

0.388
  comGC Lactococcus lactis subsp. cremoris KW2

46.341

79.612

0.369

Multiple sequence alignment    



References

[1] Fedy Morgene et al. (2023) Natural transformation and cell division delay in competent Staphylococcus aureus. Microbiology Spectrum 11(6):e0280723. [PMID: 37831481]
[2] Mais Maree et al. (2022) Natural transformation allows transfer of SCCmec-mediated methicillin resistance in Staphylococcus aureus biofilms. Nature Communications 13(1):2477. [PMID: 35513365]
[3] Annette Fagerlund et al. (2014) Staphylococcus aureus competence genes: mapping of the SigH, ComK1 and ComK2 regulons by transcriptome sequencing. Molecular Microbiology 94(3):557-79. [PMID: 25155269]
[4] Kazuya Morikawa et al. (2003) A new staphylococcal sigma factor in the conserved gene cassette: functional significance and implication for the evolutionary processes. Genes To Cells : Devoted To Molecular & Cellular Mechanisms 8(8):699-712. [PMID: 12875655]