Detailed information    

experimental Experimentally validated

Overview

Name   comEA   Type   Machinery gene
Locus tag   SA_RS08005 Genome accession   NC_002745
Coordinates   1625096..1625782 (-) Length   228 a.a.
NCBI ID   WP_001802022.1    Uniprot ID   A0A7U7EYP5
Organism   Staphylococcus aureus N315     
Function   dsDNA binding to the cell surface   
DNA binding and uptake

Function

ComEA is anchored to the membrane via an N-terminal membrane-spanning domain. Its C-terminus is located outside the membrane and is required for DNA binding and transport.


Genomic Context

Location: 1620096..1630782
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SA_RS07985 (SA1414) rpsT 1621010..1621261 (+) 252 WP_001274017.1 30S ribosomal protein S20 -
  SA_RS07990 (SA1415) holA 1621306..1622280 (-) 975 WP_001282560.1 DNA polymerase III subunit delta -
  SA_RS07995 (SA1416) comEC 1622337..1624538 (-) 2202 WP_001557362.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  SA_RS08000 (SA1417) - 1624543..1625004 (-) 462 WP_000439693.1 ComE operon protein 2 -
  SA_RS08005 (SA1418) comEA 1625096..1625782 (-) 687 WP_001802022.1 ComEA family DNA-binding protein Machinery gene
  SA_RS08010 (SA1419) - 1625822..1626538 (-) 717 WP_000084830.1 class I SAM-dependent DNA methyltransferase -
  SA_RS08015 (SA1420) rsfS 1626541..1626894 (-) 354 WP_001088020.1 ribosome silencing factor -
  SA_RS08020 (SA1421) yqeK 1626895..1627479 (-) 585 WP_001019324.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  SA_RS08025 (SA1422) nadD 1627469..1628038 (-) 570 WP_000725167.1 nicotinate (nicotinamide) nucleotide adenylyltransferase -
  SA_RS08030 (SA1423) yhbY 1628041..1628331 (-) 291 WP_000955235.1 ribosome assembly RNA-binding protein YhbY -
  SA_RS08035 (SA1424) aroE 1628335..1629141 (-) 807 WP_000666757.1 shikimate dehydrogenase -
  SA_RS08040 (SA1425) yqeH 1629155..1630255 (-) 1101 WP_001280137.1 ribosome biogenesis GTPase YqeH -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  sigH comEA positive effect
  sigH comGD positive effect
  agrC comGD positive effect
  braS comGD positive effect
  braR comGD positive effect
  vraR comGD negative effect
  agrA comGD positive effect
  comK/comK1 comGD positive effect
  vraS comGD negative effect
  agrC comGB positive effect
  braR comGB positive effect
  sigH comGB positive effect
  braS comGB positive effect
  vraS comGB negative effect
  agrA comGB positive effect
  comK/comK1 comGB positive effect
  vraR comGB negative effect
  agrC comGC positive effect
  vraS comGC negative effect
  agrA comGC positive effect
  comK/comK1 comGC positive effect
  vraR comGC negative effect
  braR comGC positive effect
  sigH comGC positive effect
  braS comGC positive effect
  agrC comGE positive effect
  vraS comGE negative effect
  agrA comGE positive effect
  comK/comK1 comGE positive effect
  vraR comGE negative effect
  braR comGE positive effect
  sigH comGE positive effect
  braS comGE positive effect
  agrC comGA positive effect
  agrA comGA positive effect
  comK/comK1 comGA positive effect
  vraR comGA negative effect
  vraS comGA negative effect
  sigH comGA positive effect
  braS comGA positive effect
  braR comGA positive effect
  agrC comGF positive effect
  agrA comGF positive effect
  comK/comK1 comGF positive effect
  vraR comGF negative effect
  vraS comGF negative effect
  sigH comGF positive effect
  braS comGF positive effect
  braR comGF positive effect
  sigH coiA positive effect
  comK/comK1 coiA positive effect
  sigH positive effect
  comK/comK1 positive effect
  comK/comK1 comEA positive effect
  sigH ssb positive effect
  comK/comK1 ssb positive effect
  sigH dprA positive effect
  comK/comK1 dprA positive effect

Sequence

Protein


Download         Length: 228 a.a.        Molecular weight: 25900.65 Da        Isoelectric Point: 9.6751

>NTDB_id=20 SA_RS08005 WP_001802022.1 1625096..1625782(-) (comEA) [Staphylococcus aureus N315]
MVLLYQFLLRYKDFLTQWKLYIISAVVLIMVLIGFIFWRQDDYTSRNFENKDTALKQSTSENNSLSKLEDVQVKDGDNSK
NKGPVYVDVKGAVKHPNVYKMTSKDRVVDLLDKAQLLDDADVSRINLSEKLTDQKMIFIPHKGQKNVEPQIEVNSVHVKN
GNTNNTKVNLNTASVSELMSVPGVGQAKANAIVEYRNQQGAFQEIDDLKKVKGFGSKTFDKLKSYFTI

Nucleotide


Download         Length: 687 bp        

>NTDB_id=20 SA_RS08005 WP_001802022.1 1625096..1625782(-) (comEA) [Staphylococcus aureus N315]
GTGGTTTTATTGTATCAATTTTTATTACGCTATAAAGATTTTTTAACTCAGTGGAAGTTATATATTATAAGTGCTGTTGT
TTTAATTATGGTATTAATTGGTTTTATATTCTGGAGACAAGATGATTATACTTCAAGAAATTTTGAAAATAAAGATACTG
CTCTGAAACAAAGCACTAGTGAAAATAATAGTTTGTCCAAATTAGAAGATGTCCAGGTCAAAGATGGAGATAATTCCAAA
AATAAAGGTCCTGTATATGTCGATGTAAAAGGTGCTGTTAAACATCCTAATGTTTATAAAATGACATCTAAGGATAGAGT
AGTTGATTTACTTGATAAAGCACAATTATTGGATGATGCAGATGTAAGTCGAATTAATTTGTCTGAAAAATTAACAGATC
AAAAAATGATTTTCATACCTCATAAAGGACAAAAGAATGTTGAACCACAAATTGAAGTAAACAGTGTGCACGTAAAAAAT
GGGAACACAAATAATACTAAAGTAAATTTAAATACCGCATCTGTATCAGAATTGATGTCTGTTCCTGGAGTAGGGCAAGC
TAAAGCTAATGCAATTGTTGAATATCGCAACCAACAAGGTGCATTTCAAGAAATTGACGATTTGAAAAAAGTAAAAGGTT
TTGGAAGTAAAACTTTTGATAAACTGAAATCTTATTTCACGATATAA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(164-227)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7U7EYP5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Staphylococcus aureus MW2

97.368

100

0.974
  Cj0011c Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

57.377

77.215

0.443
  comEA Bacillus subtilis subsp. subtilis str. 168

36.364

100

0.39
  comEA Legionella pneumophila str. Paris

38.384

94.286

0.362
  comEA Legionella pneumophila strain ERS1305867

38.384

94.286

0.362

Multiple sequence alignment    



References

[1] Mais Maree et al. (2022) Natural transformation allows transfer of SCCmec-mediated methicillin resistance in Staphylococcus aureus biofilms. Nature Communications 13(1):2477. [PMID: 35513365]
[2] Annette Fagerlund et al. (2014) Staphylococcus aureus competence genes: mapping of the SigH, ComK1 and ComK2 regulons by transcriptome sequencing. Molecular Microbiology 94(3):557-79. [PMID: 25155269]
[3] Kazuya Morikawa et al. (2003) A new staphylococcal sigma factor in the conserved gene cassette: functional significance and implication for the evolutionary processes. Genes To Cells : Devoted To Molecular & Cellular Mechanisms 8(8):699-712. [PMID: 12875655]